公开(公告)号：US09551024B2

公开(公告)日：2017-01-24

申请号：US13748516

申请日：2013-01-23

Applicant: Dow AgroSciences LLC

Inventor： Yunxing Cory Cui ,  Raina King ,  Tina Marie Kaiser ,  Andrew E. Robinson ,  Dayakar Pareddy ,  Sandra Grace Toledo ,  Leon B. Braxton ,  David M. Anderson

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/82 ,  A01H1/04

CPC classification number: C12Q1/6813 ,  A01H1/04 ,  C12N15/8274 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/16

Abstract: Cotton event pDAB4468.18.07.1 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.

Abstract translation: 棉花事件pDAB4468.18.07.1包含含有编码aad-12和pat的基因的基因表达盒，为含有该事件的棉花作物提供除草剂耐受性以及用于作物保护的有效方法。 本发明的实施方案提供了与多核苷酸相关的事件检测方法。

公开(公告)号：US20150335017A1

公开(公告)日：2015-11-26

申请号：US14410762

申请日：2013-06-25

Applicant: DOW AGROSCIENCES LLC

Inventor： Nathan Bard ,  Gregory A. Bradfisch ,  Yunxing Cory Cui ,  James E. Dripps ,  Thomas Hoffman ,  Dayakar Pareddy ,  Dawn M. Parkhurst ,  Sandra G. Toledo ,  Barry Wiggins ,  Ning Zhou ,  Aaron T. Woosley

IPC: A01N43/16 ,  C12N15/82 ,  A01H5/10

CPC classification number: A01N43/16 ,  A01H5/10 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

Abstract: Soybean event 9582.816.15.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. Embodiments of the present disclosure relate to a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated soybean event pDAB9582.816.15.1, comprising cry1F v3 (cry1F), cry1Ac synpro (cry1Ac) and pat v6 (pat), as described herein, inserted into a specific site within the genome of a soybean cell. Representative soybean seed has been deposited with American Type Culture Collection (ATCC) with the Accession No. ATCC Deposit No. PTA-12588. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome.

Abstract translation: 大豆事件9582.816.15.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。 本公开的实施方案涉及如本文所述的新的耐昆虫抗性和除草剂耐受转基因大豆转化事件，称为大豆事件pDAB9582.816.15.1，包含cry1F v3（cry1F），cry1Ac synpro（cry1Ac）和pat v6（pat） 插入大豆细胞基因组内的特定位点。 代表性的大豆种子已经存入具有登录号ATCC保藏号PTA-12588的美国典型培养物保藏中心（ATCC）。 含有该事件的大豆植物的DNA包括本文所述的连接/侧翼序列，其表征插入的DNA在大豆基因组内的位置。

公开(公告)号：US20140173774A1

公开(公告)日：2014-06-19

申请号：US14134883

申请日：2013-12-19

Applicant: Dow AgroSciences LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley Smith ,  Rodrigo Sarria-Milan

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

Abstract translation: 公开了一种用于土壤杆菌介导的大豆种系转化的方法，包括用含有转基因的根癌土壤杆菌感染具有一部分胚轴的分裂大豆种子。 所述方法还可以包括通过在选择培养基上体外包含部分胚胎轴的裂解大豆种子转化产生的外植体再生。

公开(公告)号：US10455834B2

公开(公告)日：2019-10-29

申请号：US14410762

申请日：2013-06-25

Applicant: DOW AGROSCIENCES LLC

Inventor： Nathan Bard ,  Gregory A. Bradfisch ,  Yunxing Cory Cui ,  James E. Dripps ,  Thomas Hoffman ,  Dayakar Pareddy ,  Dawn M. Parkhurst ,  Sandra G. Toledo ,  Barry Wiggins ,  Ning Zhou ,  Aaron T. Woosley

IPC: C12N15/82 ,  A01N43/16 ,  A01H5/10

Abstract: Soybean event 9582.816.15.1 comprises genes encoding Cry1 F, Cry1 Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. Embodiments of the present disclosure relate to a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated soybean event pDAB9582.816.15.1, comprising cry1 F v3 (cry1 F), cry1 Ac synpro (cry1 Ac) and pat v6 (pat), as described herein, inserted into a specific site within the genome of a soybean cell. Representative soybean seed has been deposited with American Type Culture Collection (ATCC) with the Accession No. ATCC Deposit No. PTA-12588. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome.

公开(公告)号：US10214746B2

公开(公告)日：2019-02-26

申请号：US14505060

申请日：2014-10-02

Applicant: Dow AgroSciences LLC

Inventor： Sivarama Reddy Chennareddy ,  Dayakar Pareddy ,  Jayakumar Pon Samuel ,  Rodrigo Sarria-Milan ,  Toby Cicak

IPC: C12N15/82

Abstract: The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

公开(公告)号：US09353381B2

公开(公告)日：2016-05-31

申请号：US13748497

申请日：2013-01-23

Applicant: Dow AgroSciences LLC

Inventor： Yunxing Cory Cui ,  Raina King ,  Tina Marie Kaiser ,  Andrew E. Robinson ,  Dayakar Pareddy ,  Sandra Grace Toledo ,  Leon B. Braxton ,  David M. Anderson

IPC: C12Q1/68 ,  C12P19/34 ,  C12N15/82 ,  C12N9/02

CPC classification number: C12N15/8274 ,  A01H1/02 ,  A01N37/38 ,  A01N39/04 ,  A01N43/40 ,  A01N57/20 ,  C12N9/0069 ,  C12N9/0071 ,  C12Q1/6876 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Cotton event pDAB4468.19.10.3 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.

Abstract translation: 棉花事件pDAB4468.19.10.3包含基因表达盒，其含有编码aad-12和pat的基因，为含有该事件的棉花作物提供除草剂耐受性，以及用于作物保护的有效方法。 本发明的实施方案提供了与多核苷酸相关的事件检测方法。

公开(公告)号：US20140201873A1

公开(公告)日：2014-07-17

申请号：US14223249

申请日：2014-03-24

Applicant: DOW AGROSCIENCES LLC

Inventor： Nathan Bard ,  Gregory A. Bradfisch ,  Yunxing C. Cui ,  James E. Dripps ,  Thomas Hoffman ,  Dayakar Pareddy ,  Dawn M. Parkhurst ,  Sandra G. Toledo ,  Barry Wiggins ,  Ning Zhou

IPC: C12N15/82 ,  C12Q1/68

CPC classification number: C12N15/8286 ,  A01N37/38 ,  A01N37/44 ,  A01N65/20 ,  C12N15/8209 ,  C12N15/8274 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/13 ,  Y02A40/162

Abstract: Soybean event 9582.814.19.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

Abstract translation: 大豆事件9582.814.19.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。

公开(公告)号：US20130191938A1

公开(公告)日：2013-07-25

申请号：US13748402

申请日：2013-01-23

Applicant: DOW AGROSCIENCES LLC

Inventor： Yunxing C. Cui ,  Raina King ,  Tina M. Kaiser ,  Andrew E. Robinson ,  Dayakar Pareddy ,  Sandra G. Toledo ,  Leon B. Braxton ,  David M. Anderson

IPC: C12N15/82

CPC classification number: C12Q1/6813 ,  A01H1/04 ,  C12N15/8274 ,  C12N15/8277 ,  C12Q1/6895 ,  C12Q2600/16

Abstract: Cotton event pDAB4468.18.07.1 comprises genes encoding AAD-12 and PAT, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection.

Abstract translation: 棉花事件pDAB4468.18.07.1包括编码AAD-12和PAT的基因，为含有该事件的棉花作物提供除草剂耐受性，以及用于作物保护的有效方法。