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    Soybean transformation method
    4.
    发明授权
    Soybean transformation method 有权

    公开(公告)号：US10214746B2

    公开(公告)日：2019-02-26

    申请号：US14505060

    申请日：2014-10-02

    申请人： Dow AgroSciences LLC

    发明人： Sivarama Reddy Chennareddy ,  Dayakar Pareddy ,  Jayakumar Pon Samuel ,  Rodrigo Sarria-Milan ,  Toby Cicak

    IPC分类号： C12N15/82

    摘要： The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

    Synthetic bi-directional plant promoter
    8.
    发明授权
    Synthetic bi-directional plant promoter 有权

    公开(公告)号：US09988638B2

    公开(公告)日：2018-06-05

    申请号：US14938643

    申请日：2015-11-11

    申请人： Dow AgroSciences LLC

    发明人： Sandeep Kumar ,  Toby Cicak ,  Heather Leigh Robinson ,  Dayakar Reddy Pareddy ,  Wei Chen

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216

    摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.

    SYNTHETIC BI-DIRECTIONAL PLANT PROMOTER
    9.
    发明申请
    SYNTHETIC BI-DIRECTIONAL PLANT PROMOTER 有权
    合成双向植物促进剂

    公开(公告)号：US20160130595A1

    公开(公告)日：2016-05-12

    申请号：US14938643

    申请日：2015-11-11

    申请人： Dow AgroSciences LLC

    发明人： Sandeep Kumar ,  Toby Cicak ,  Heather Leigh Robinson ,  Dayakar Reddy Pareddy ,  Wei Chen

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216

    摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a minimal core promoter element from a Arabidopsis thaliana Ubiquitin-10 gene promoter or Cassava Vein Mosaic Virus promoter, and the full-length nucleotide sequence elements from a Cassava Vein Mosaic Virus promoter. Some embodiments relate to a synthetic CsVMV bi-directional promoter that functions in plants to promote transcription of two operably linked nucleotide sequences.

    摘要翻译： 本公开涉及用于促进植物或植物细胞中核苷酸序列的转录的组合物和方法，其使用来自拟南芥泛素-10基因启动子或木薯浆静脉花叶病毒启动子的最小核心启动子元件，以及全长核苷酸序列元件 从木薯静脉花叶病毒启动子。 一些实施方案涉及在植物中起作用以促进两个可操作连接的核苷酸序列的转录的合成的CsVMV双向启动子。