公开(公告)号：US20170303486A1

公开(公告)日：2017-10-26

申请号：US15518081

申请日：2015-10-09

Applicant: Dow AgroSciences LLC

Inventor： Tristan E. Coram ,  Susan M. Jayne ,  Diaa Alabed ,  Stephen Foulk ,  Rajat Aggarwal ,  Natae Daniels

IPC: A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  G01N33/53 ,  G01N33/00

CPC classification number: A01H1/04 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8205 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  G01N33/00

Abstract: This invention relates to methods for identifying maize plants that having increased culturability and/or transformability. The methods use molecular markers to identify and to select plants with increased culturability and/or transformability or to identify and deselect plants with decreased culturability and/or transformability. Maize plants generated by the methods of the invention are also a feature of the invention.

公开(公告)号：US20170051304A1

公开(公告)日：2017-02-23

申请号：US15232876

申请日：2016-08-10

Applicant: Dow AgroSciences LLC

Inventor： Timothy D. Hey ,  Xu Xiaoping ,  Todd P. Glancy ,  Diaa Alabed ,  Sarah E. Worden ,  Nick X. Wang ,  Carla Ausmus ,  Shao-Ching Hung

IPC: C12N15/82 ,  A01N37/44 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Engineered and modified Cry6Aa insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Abstract translation: 公开了工程化和修饰的Cry6Aa杀虫毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。

公开(公告)号：US09422569B2

公开(公告)日：2016-08-23

申请号：US13674606

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建物包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US09752155B2

公开(公告)日：2017-09-05

申请号：US14472977

申请日：2014-08-29

Applicant: Dow AgroSciences, LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8216 ,  C07K14/415

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

公开(公告)号：US20170029833A1

公开(公告)日：2017-02-02

申请号：US15212000

申请日：2016-07-15

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry R. Wright ,  Manju Gupta

IPC: C12N15/82 ,  C07K14/005

CPC classification number: C12N15/8216 ,  C07K14/005 ,  C12N15/8222 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 aerie or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1通气或其功能等同物的最小核心启动子元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US09453235B2

公开(公告)日：2016-09-27

申请号：US13674712

申请日：2012-11-12

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。