公开(公告)号：US20240307524A1

公开(公告)日：2024-09-19

申请号：US18576969

申请日：2022-07-07

申请人： Luxembourg Institute of Health (LIH)

发明人： Xavier Dervillez ,  Catherine Léonard ,  Markus Wolfgang Ollert

IPC分类号： A61K39/215 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00

CPC分类号： A61K39/215 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/00022 ,  C12N2770/00034

摘要： The present invention provides multimeric protein complex comprising three polypeptides each comprising N- to C-terminally: (i) a receptor-binding domain (RBD) of an S1 subunit of an S protein of a coronavirus, (ii) optionally a S2 subunit of an S protein of a coronavirus; and (iii) a multimerization domain comprising a collagen-like region (CLR) of ficolin-2, wherein the multimerization domain enables the assembly of the polypeptides into a multimeric protein complex. The present invention further provides polynucleotides encoding the polypeptides of the multimeric protein complex, expression vectors, pharmaceutical compositions and uses of the multimeric protein complexes, such as a vaccine.

公开(公告)号：US20240271147A1

公开(公告)日：2024-08-15

申请号：US18568456

申请日：2022-06-10

申请人： University of Cape Town

发明人： Ann Elizabeth MEYERS ,  Edward Peter RYBICKI ,  Inga Isabel Hitzeroth ,  Susan Jennifer Dennis

IPC分类号： C12N15/82 ,  C07K14/005

CPC分类号： C12N15/8201 ,  C07K14/005 ,  C12N2770/00022 ,  C12N2770/00023 ,  C12N2770/00034 ,  C12N2770/00042

摘要： Provided herein is a method for stabilising a single stranded RNA (ssRNA) by encapsidation of the ssRNA with a tobamovirus coat protein to obtain a pseudovirion (PsV), the method comprising expressing a tobamovirus coat protein and the ssRNA comprising a tobamovirus encapsidation origin (OriA), wherein the expressed tobamovirus coat protein interacts with the OriA sequence on the ssRNA to initiate encapsidation of the ssRNA by the tobamovirus coat protein, thereby forming a pseudovirion. The PsVs produced according to the method can be used as a diagnostic control composition, where the ssRNA is a sequence detected by a molecular diagnostic assay. The pseudovirions may also be used as a vaccine to elicit an immune response in a subject, and in pharmaceutical compositions to be administered to a subject.

公开(公告)号：US20240209382A1

公开(公告)日：2024-06-27

申请号：US18516243

申请日：2023-11-21

申请人： Purdue Research Foundation ,  University of Delaware

发明人： Kevin SOLOMON ,  Kok Zhi LEE ,  Akash VAIDYA ,  Mruthula RAMMOHAN

IPC分类号： C12N15/70 ,  B82Y5/00 ,  C07K14/005 ,  C12N7/00

CPC分类号： C12N15/70 ,  C07K14/005 ,  C12N7/00 ,  B82Y5/00 ,  C12N2770/00022 ,  C12N2770/00023

摘要： Methods and nucleic acid sequences for the synthesis of biotemplates in a non-plant based expression system are provided. Such biotemplates include Barley stripe mosaic virus viral-like particles (BSMV-VLPs) that are capable of self-assembly due to being operatively linked with an origin of self-assembly with the Barley stripe mosaic virus capsid protein (BSMV-CP). Also provided are BSMV-VLPs that are capable of self-assembly due one or more site-directed mutations on the BSMV-CP, and BSMV-VLPs that exhibit enhanced stability due to such site-directed mutation(s).

公开(公告)号：US20240207390A1

公开(公告)日：2024-06-27

申请号：US18556556

申请日：2022-12-23

申请人： WestVac Biopharma Co., Ltd.

发明人： Guobo SHEN ,  Xiawei WEI ,  Yuquan WEI ,  Li YANG

IPC分类号： A61K39/215 ,  C07K14/005 ,  C12N5/07 ,  C12N7/00 ,  C12R1/91

CPC分类号： A61K39/215 ,  C07K14/005 ,  C12N5/0601 ,  C12N7/00 ,  C12N2770/00022 ,  C12N2770/20034 ,  C12R2001/91

摘要： The invention pertains to the technical fields of genetic engineering and cell engineering, and in particular relates to a rhabdovirus-negative Spodoptera frugiperda insect cell line, and screening, identification and application thereof. According to the invention, the rhabdovirus-negative Spodoptera frugiperda insect cell line WSK-Sf9, with a CCTCC accession number C202246, is obtained through screening and identification. The cell line is verified through various high-sensitivity test methods such as nested PCR, transcriptome next-generation sequencing, real-time fluorescence quantitative PCR and TAQMAN probe-based real-time PCR, and finally obtained the Sf-rhabdovirus-negative Spodoptera frugiperda insect cell line WSK-Sf9. The cell is tested for sterility, mycoplasma, exogenous virus and tumorigenicity according to pharmacopeial requirements, and the results show that all indicators satisfy the requirements, and the cell can be used in or for the production of recombinant proteins and recombinant protein vaccines based on the baculovirus expression system.

公开(公告)号：US20230272413A1

公开(公告)日：2023-08-31

申请号：US18066191

申请日：2022-12-14

申请人： The United States of America, as represented by the Secretary of Agriculture

发明人： MICHELLE L. HECK ,  JENNIFER R. WILSON ,  CARL J. SCHILTZ ,  JOSHUA S. CHAPPIE ,  MYFANWY C. ADAMS

IPC分类号： C12N15/82 ,  C07K14/005 ,  C12N7/00 ,  A01N63/50 ,  G01N33/569 ,  C07K16/10

CPC分类号： C12N15/8286 ,  C07K14/005 ,  C12N7/00 ,  A01N63/50 ,  G01N33/56983 ,  C07K16/10 ,  C12N2770/00022 ,  C12N2770/00021 ,  G01N2333/08 ,  C07K2317/569

摘要： Disclosed herein are plant-based, molecular and diagnostic tools that can be used to block aphid transmission of poleroviruses, including stabilized proteins for expression in transgenic plants and/or in formulations for direct plant delivery. Further disclosed are proteins that can kill aphids and methods to produce these proteins. Antibodies and useful for diagnostic and therapeutic uses against poleroviruses.

公开(公告)号：US11712462B2

公开(公告)日：2023-08-01

申请号：US17245213

申请日：2021-04-30

申请人： REALTA HOLDINGS, LLC

发明人： Neel K. Krishna ,  Kenji Cunnion

IPC分类号： A61K38/00 ,  A61K38/43 ,  A61P37/06 ,  A61K47/10 ,  A61K9/00 ,  A61K38/08 ,  C07K14/47 ,  C07K14/005

CPC分类号： A61K38/43 ,  A61K9/0019 ,  A61K38/005 ,  A61K38/08 ,  A61K47/10 ,  A61P37/06 ,  C07K14/005 ,  C07K14/4703 ,  C12N2770/00022 ,  C12N2770/00033

摘要： A method of treating systemic lupus erythematosus in a subject is provided in which a therapeutically effective amount of PIC1 is administered to the subject. A method of treating transfusion-related acute lung injury is also provided where a therapeutically effective amount of PIC1 is administered to the subject. PIC1 can modulate immune complex activation of the complement system and NET formation in the subject. PIC1 can also inhibit myeloperoxidase (MPO) activity in the subject.

公开(公告)号：US11649453B2

公开(公告)日：2023-05-16

申请号：US17211595

申请日：2021-03-24

申请人： Bio-Rad Laboratories, Inc.

发明人： Xiao-Song Gong ,  Yan Wang

IPC分类号： C12N9/10 ,  C12N9/22 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6844

CPC分类号： C12N15/1096 ,  C12N9/22 ,  C12Q1/686 ,  C12N2770/00022 ,  C12Q1/6844 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2521/327

摘要： Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.

公开(公告)号：US20170121725A1

公开(公告)日：2017-05-04

申请号：US15303174

申请日：2015-03-11

申请人： CHUNG-ANG UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION

发明人： Gung Pyo Lee ,  Sun Ju Rhee

IPC分类号： C12N15/82 ,  C12N7/00

CPC分类号： C12N15/8218 ,  C07K14/005 ,  C12N7/00 ,  C12N15/8216 ,  C12N15/86 ,  C12N2770/00022 ,  C12N2770/00043

摘要： The purpose of the present invention is to develop a cucumber fruit mottle mosaic virus vector so as to enable a study of gene function through gene silencing phenomenon in Cucurbitaceae plants, which has been difficult to study in the past, and to enable the study in fruits as well through stable expression. Furthermore, the present invention establishes a system that enables expression of a heterologous protein in plants, thereby, for the first time, providing a vector applicable to both the gene silencing phenomenon and protein expression in Cucurbtaceae plants.

公开(公告)号：US20130133109A1

公开(公告)日：2013-05-23

申请号：US13752605

申请日：2013-01-29

申请人： State of Oregon Acting by and through the State Board of Higher Education on behalf of Oregon State University

发明人： Valerian V. Dolja ,  Valera V. Peremyslov

IPC分类号： C12N15/82

CPC分类号： C12N15/8239 ,  C07K14/005 ,  C12N15/8203 ,  C12N2770/00022

摘要： The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.

摘要翻译： 本公开涉及用于将核苷酸递送至植物的基于布氏病毒的载体的开发和使用。 具体地，本公开提供了基于葡萄叶卷叶病毒-2的病毒载体，用于在植物，特别是葡萄植物中递送和表达基因。 还考虑了制备和使用这些载体的方法以及由这些载体转化的植物。

公开(公告)号：US20100333230A1

公开(公告)日：2010-12-30

申请号：US12865708

申请日：2009-01-29

申请人： Valerian V. Dolja ,  Valera V. Peremyslov

发明人： Valerian V. Dolja ,  Valera V. Peremyslov

IPC分类号： A01H1/00 ,  C12N15/63 ,  C12N5/10 ,  A01H5/00 ,  C12N15/82

CPC分类号： C12N15/8239 ,  C07K14/005 ,  C12N15/8203 ,  C12N2770/00022

摘要： The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.

摘要翻译： 本公开涉及用于将核苷酸递送至植物的基于布氏病毒的载体的开发和使用。 具体地，本公开提供了基于葡萄叶卷叶病毒-2的病毒载体，用于在植物，特别是葡萄植物中递送和表达基因。 还考虑了制备和使用这些载体的方法以及由这些载体转化的植物。