公开(公告)号：US10793850B2

公开(公告)日：2020-10-06

申请号：US14384192

申请日：2013-03-12

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie Wiessenhaan ,  Catharina Petronella Antonia Maria Kolen ,  Bernard Meijrink ,  Viktor Marius Boer ,  Johannes Andries Roubos ,  Yvonne Johannes Odilia Arendsen

IPC: C12N15/90 ,  C12N15/10

Abstract: The present invention relates to a method for carrying out recombination at a target locus.

公开(公告)号：US09738890B2

公开(公告)日：2017-08-22

申请号：US14386354

申请日：2013-03-27

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries Roubos ,  Herman Jan Pel ,  Bernard Meijrink

IPC: C12N15/10 ,  C12N15/66 ,  C12N15/90

CPC classification number: C12N15/1024 ,  C12N15/66 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2800/80

Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.

公开(公告)号：US20150291986A1

公开(公告)日：2015-10-15

申请号：US14441902

申请日：2013-11-25

Applicant: DSM IP ASSETS B.V.

Inventor： Zheng Zhao ,  Bernard Meijrink ,  Robertus Antonius Mijndert Van Der Hoeven ,  Liang Wu ,  Johannes Andries Roubos

IPC: C12P7/46 ,  C12P7/62 ,  C12N9/88 ,  C12N15/81

CPC classification number: C12P7/46 ,  C12N9/88 ,  C12N15/81 ,  C12P7/62 ,  C12Y401/01006

Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.

Abstract translation: 本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。 本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞：编码具有顺式乌头酸脱羧酶活性的多肽的核酸; 以及编码催化向乙酰辅酶A反应的多肽的核酸。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。

公开(公告)号：US20150050696A1

公开(公告)日：2015-02-19

申请号：US14386354

申请日：2013-03-27

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Andries Roubos ,  Herman Jan Pel ,  Bernard Meijrink

IPC: C12N15/10

CPC classification number: C12N15/1024 ,  C12N15/66 ,  C12N15/90 ,  C12N2800/30 ,  C12N2800/40 ,  C12N2800/50 ,  C12N2800/80

Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.

Abstract translation: 本发明涉及一种基于限制酶消化和通过切割位点连接的方法，从而制备两个或多个标准化表达盒。