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    ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION
    3.
    发明申请
    ITACONIC ACID AND ITACONATE METHYLESTER PRODUCTION 审中-公开
    ITACONIC ACID和ITACONATE METHYLESTER生产

    公开(公告)号：US20150291986A1

    公开(公告)日：2015-10-15

    申请号：US14441902

    申请日：2013-11-25

    Applicant: DSM IP ASSETS B.V.

    Inventor： Zheng Zhao ,  Bernard Meijrink ,  Robertus Antonius Mijndert Van Der Hoeven ,  Liang Wu ,  Johannes Andries Roubos

    IPC: C12P7/46 ,  C12P7/62 ,  C12N9/88 ,  C12N15/81

    CPC classification number: C12P7/46 ,  C12N9/88 ,  C12N15/81 ,  C12P7/62 ,  C12Y401/01006

    Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate or 1-methyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.

    Abstract translation: 本发明涉及能够产生衣康酸4-甲酯或衣康酸1-甲酯中的一种或多种的重组酵母细胞。 本发明还涉及能够产生衣康酸并且过表达的重组酵母细胞：编码具有顺式乌头酸脱羧酶活性的多肽的核酸; 以及编码催化向乙酰辅酶A反应的多肽的核酸。 这些重组酵母细胞可用于生产衣康酸，衣康酸4-甲酯或衣康酸1-甲酯的方法。

    Acetyl-CoA producing enzymes in yeast
    4.
    发明授权
    Acetyl-CoA producing enzymes in yeast 有权

    公开(公告)号：US10927399B2

    公开(公告)日：2021-02-23

    申请号：US14045683

    申请日：2013-10-03

    Applicant: DSM IP ASSETS B.V.

    Inventor： Ulrike Maria Mueller ,  Liang Wu ,  Lourina Madeleine Raamsdonk ,  Aaron Adriaan Winkler

    IPC: C12N1/16 ,  C12N9/04 ,  C12Q1/32 ,  C07K14/33 ,  C12N9/02 ,  C12P7/16

    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

    ACETYL-COA PRODUCING ENZYMES IN YEAST
    5.
    发明申请
    ACETYL-COA PRODUCING ENZYMES IN YEAST 有权

    公开(公告)号：US20210054433A1

    公开(公告)日：2021-02-25

    申请号：US17081187

    申请日：2020-10-27

    Applicant: DSM IP ASSETS B.V.

    Inventor： Ulrike Maria MUELLER ,  Liang Wu ,  Lourina Madeleine Raamsdonk ,  Aaron Winkler

    IPC: C12Q1/32 ,  C07K14/33 ,  C12N9/02 ,  C12P7/16

    Abstract: The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) of a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grown on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

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