公开(公告)号：US11225647B2

公开(公告)日：2022-01-18

申请号：US16345129

申请日：2017-10-26

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius Boer ,  Priscilla Zwartjens ,  Johannes Gustaaf Ernst Van Leeuwen

IPC: C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12P15/00 ,  C12P19/56 ,  C12N15/52 ,  C12P7/42

Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions
92, 100 or 235
said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

公开(公告)号：US11203764B2

公开(公告)日：2021-12-21

申请号：US16938483

申请日：2020-07-24

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Gustaaf Ernst Van Leeuwen ,  Priscilla Zwartjens ,  Viktor Marius Boer

IPC: C12N15/81 ,  C12N9/02 ,  C12P19/56 ,  C12N9/88 ,  C12N15/70

Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

公开(公告)号：US10913938B2

公开(公告)日：2021-02-09

申请号：US16318776

申请日：2017-07-27

Applicant: DSM IP ASSETS B.V.

Inventor： Johannes Gustaaf Ernst Van Leeuwen ,  Loes Elizabeth Bevers

IPC: C12N9/02 ,  C12N9/42 ,  C12N1/14 ,  C12N15/52 ,  C12N15/80 ,  C12P7/10 ,  C12P19/14

Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.

公开(公告)号：US10604743B2

公开(公告)日：2020-03-31

申请号：US15558133

申请日：2016-03-16

Applicant: DSM IP ASSETS B.V.

Inventor： Viktor Marius Boer ,  Johannes Gustaaf Ernst Van Leeuwen ,  Priscilla Zwartjens ,  Catharina Petronella Antonia Maria Kolen

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/10 ,  C12P5/00 ,  C12P19/56 ,  A23L27/30 ,  A23L2/60

Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.