公开(公告)号：US20070010024A1

公开(公告)日：2007-01-11

申请号：US11486416

申请日：2006-07-12

Applicant: Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

Inventor： Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

IPC: G01N33/00

CPC classification number: G01N33/6848 ,  H01J49/00 ,  Y10T436/17 ,  Y10T436/173845

Abstract: Method of identification and quantitative analysis of primary and/or secondary amine(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said amine with a stable isotope labeled reagent, and is added to a sample containing said amine. Said amine in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said amine are made from a plot of ion ratio of said converted amine to said internal standard versus amine concentration.

公开(公告)号：US20050070022A1

公开(公告)日：2005-03-31

申请号：US10675763

申请日：2003-09-30

Applicant: Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

Inventor： Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

IPC: G01N33/00

CPC classification number: H01J49/0009 ,  C10L1/1857 ,  Y10T436/13 ,  Y10T436/173845 ,  Y10T436/200833 ,  Y10T436/24 ,  Y10T436/25125

Abstract: Method of identification and quantitative analysis of aldehyde(s) and/or ketone(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said aldehyde(s) and/or ketone(s) with a stable isotope labeled reagent, and is added to a sample containing said aldehyde(s) and/or ketone(s). Said aldehyde(s) and/or ketone(s) in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said aldehyde(s) and/or ketone(s) are made from a plot of ion ratio of said converted aldehyde and/or ketone to said internal standard versus aldehyde and/or ketone concentration.

Abstract translation: 提供了使用稳定同位素标记的内标通过质谱鉴定和定量分析样品中的醛和/或酮的方法。 所述内标是通过将所述醛和/或酮的真实样品与稳定同位素标记的试剂反应制备的，并加入到含有所述醛和/或酮的样品中， 。 然后使用非标记试剂将所述样品中的所述醛和/或酮定量地转化为具有相同结构的化合物，除了稳定的同位素原子，如所述内标的那样。 然后提取所述样品，并通过质谱法分析提取物。 所述醛和/或酮的鉴定和定量由所述转化的醛和/或酮与所述内标的离子比对醛和/或酮浓度的曲线图来制备。

公开(公告)号：US20050014279A1

公开(公告)日：2005-01-20

申请号：US10619709

申请日：2003-07-14

Applicant: Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

Inventor： Hoa Nguyen ,  Trinh Nguyen ,  Duc Nguyen

IPC: A61M36/14 ,  G01N33/00 ,  G01N33/487

CPC classification number: G01N33/6848 ,  H01J49/00 ,  Y10T436/17 ,  Y10T436/173845

Abstract: Method of identification and quantitative analysis of primary and/or secondary amine(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said amine with a stable isotope labeled reagent, and is added to a sample containing said amine. Said amine in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said amine are made from a plot of ion ratio of said converted amine to said internal standard versus amine concentration.

Abstract translation: 提供了使用稳定同位素标记的内标通过质谱鉴定和定量分析样品中的一级或二级胺的方法。 所述内标是通过将所述胺的真实样品与稳定同位素标记的试剂反应制备的，并加入含有所述胺的样品中。 然后使用非标记试剂将所述样品中的所述胺定量转化为具有相同结构的化合物，除了稳定的同位素原子，如所述内标的那样。 然后提取所述样品，并通过质谱法分析提取物。 所述胺的鉴定和定量由所述转化的胺与所述内标的离子比对胺浓度的曲线图来制备。