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24 条记录 (耗时 0.041 秒)

    Determination of AM-binding proteins and the association of adrenomedullin (AM) therewith
    12.
    发明申请
    Determination of AM-binding proteins and the association of adrenomedullin (AM) therewith 审中-公开
    AM结合蛋白的测定和肾上腺髓质素（AM）与其结合

    公开(公告)号：US20070025915A1

    公开(公告)日：2007-02-01

    申请号：US11530411

    申请日：2006-09-08

    申请人： Frank Cuttitta ,  Ted Elsasser ,  Alfredo Martinez ,  Ruben Pio

    发明人： Frank Cuttitta ,  Ted Elsasser ,  Alfredo Martinez ,  Ruben Pio

    IPC分类号： A61K51/00 ,  G01N33/574 ,  A61K39/395 ,  C07K16/30 ,  C07K16/46

    CPC分类号： C07K16/18 ,  A61K38/1709 ,  A61K2039/505 ,  G01N33/74 ,  G01N2500/00

    摘要： The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.

    摘要翻译： 本发明提供了分离，鉴定和纯化肾上腺髓质素（AM）结合蛋白的方法。 此外，提供了利用纯化的AM结合蛋白或其功能部分来诊断，治疗和监测AM相关疾病的方法，例如与异常升高的AM水平相关的疾病或病症。 此外，本发明提供了AM和特异性AM结合蛋白1（AMBP-1）之间新鉴定的复合物; 其在本文中被分离和鉴定为因子H（fH）。 本发明还提供AM / AMBP复合物，特别是AM / FH复合物和与该复合物特异性反应的抗体。 进一步提供了使用抗AM / fH抗体鉴定和纯化AM和AM结合蛋白的复合物的方法，以及使用包含这些抗体的组合物治疗诸如癌症或糖尿病的病症的方法。 本发明另外提供用于鉴定抑制AM，因子H或AM /因子H复合物的功能的拮抗剂的方法。 本发明还提供了使用这些拮抗剂治疗诸如癌症或糖尿病的病症的方法。

    Vasoregulating compounds and methods of their use
    13.
    发明申请
    Vasoregulating compounds and methods of their use 失效
    减压化合物及其使用方法

    公开(公告)号：US20050261179A1

    公开(公告)日：2005-11-24

    申请号：US10529118

    申请日：2003-10-03

    申请人： Frank Cuttitta ,  Alfredo Martinez ,  William Stetler-Stevenson ,  Edward Unsworth ,  Juan Saavedra

    发明人： Frank Cuttitta ,  Alfredo Martinez ,  William Stetler-Stevenson ,  Edward Unsworth ,  Juan Saavedra

    IPC分类号： A61B20060101 ,  A61B1/00 ,  A61K31/137 ,  A61K38/10 ,  A61K51/00 ,  A61M36/14 ,  A61P9/12 ,  A61P9/14 ,  G01N33/53

    CPC分类号： A61K38/22 ,  A61K31/137 ,  G01N33/5088 ,  G01N2500/00

    摘要： Methods and compounds are described for regulating blood pressure in a subject. Specific embodiments are methods for reversing vasodilation of blood vessels, by administering to a subject a therapeutically effective amount peptide AM(11-22). The vasoconstrictor can be used for a variety of purposes, including hemostasis or the treatment of shock, for example vasodilatory shock syndromes such as septic shock. Other specific embodiments are methods for reversing vasoconstriction of blood vessels, by administering to a subject a therapeutically effect amount of an inhibitor of AM(11-22), sufficient to reduce hypertension in the subject. Compounds and pharmaceutical compositions are also provided, as are kits.

    摘要翻译： 描述了用于调节受试者血压的方法和化合物。 具体实施方案是通过向受试者施用治疗有效量的肽AM（11-22）来逆转血管的血管舒张的方法。 血管收缩剂可用于各种目的，包括止血或治疗休克，例如血管舒张性休克综合征，如脓毒性休克。 其他具体实施方案是通过向受试者施用足以降低受试者的高血压的治疗有效量的AM（11-22）抑制剂的方法来逆转血管的血管收缩。 还提供了化合物和药物组合物，试剂盒也是如此。

    Determination of AM-binding proteins and the association of adrenomedullin (AM) therewith
    14.
    发明授权
    Determination of AM-binding proteins and the association of adrenomedullin (AM) therewith 有权
    AM结合蛋白的测定和肾上腺髓质素（AM）与其结合

    公开(公告)号：US07993857B2

    公开(公告)日：2011-08-09

    申请号：US12638747

    申请日：2009-12-15

    申请人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

    发明人： Frank Cuttitta ,  Ted H. Elsasser ,  Alfredo Martinez ,  Rubén Pío

    IPC分类号： G01N1/00 ,  G01N33/53

    CPC分类号： C07K16/18 ,  A61K38/1709 ,  A61K2039/505 ,  G01N33/74 ,  G01N2500/00

    摘要： The present invention provides methods for the isolation, identification, and purification of adrenomedullin (AM)-binding proteins. Also, provided are methods for utilizing the purified AM-binding proteins, or functional portions thereof, to diagnose, treat, and monitor AM-related diseases, for example, diseases or disorders associated with abnormally elevated AM levels. In addition, the present invention provides a newly identified complex between AM and a specific AM-binding protein 1 (AMBP-1); which has been isolated and identified herein as factor H (fH). The invention also provides AM/AMBP complexes, particularly AM/FH complexes, and antibodies specifically reactive with this complexes. Further provided are methods for identifying and purifying complexes of AM and an AM binding protein using anti-AM/fH antibodies, and methods for treating conditions such as cancer or diabetes utilizing compositions comprising these antibodies. The present invention additionally provides methods for identifying antagonists agents that inhibit the function of AM, factor H, or the AM/factor H complex. The invention also provides methods for treating conditions such as cancer or diabetes using these antagonist agents.

    摘要翻译： 本发明提供了分离，鉴定和纯化肾上腺髓质素（AM）结合蛋白的方法。 此外，提供了利用纯化的AM结合蛋白或其功能部分来诊断，治疗和监测AM相关疾病的方法，例如与异常升高的AM水平相关的疾病或病症。 此外，本发明提供了AM和特异性AM结合蛋白1（AMBP-1）之间新鉴定的复合物; 其在本文中被分离和鉴定为因子H（fH）。 本发明还提供AM / AMBP复合物，特别是AM / FH复合物和与该复合物特异性反应的抗体。 进一步提供了使用抗AM / fH抗体鉴定和纯化AM和AM结合蛋白的复合物的方法，以及使用包含这些抗体的组合物治疗诸如癌症或糖尿病的病症的方法。 本发明另外提供用于鉴定抑制AM，因子H或AM /因子H复合物的功能的拮抗剂的方法。 本发明还提供了使用这些拮抗剂治疗诸如癌症或糖尿病的病症的方法。

    Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
    18.
    发明申请
    Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons 审中-公开
    基于扩增子的不同融合温度的多重实时PCR定量方法和软件

    公开(公告)号：US20050053950A1

    公开(公告)日：2005-03-10

    申请号：US10658602

    申请日：2003-09-08

    申请人： Enrique Zudaire Ubani ,  Frank Cuttitta

    发明人： Enrique Zudaire Ubani ,  Frank Cuttitta

    IPC分类号： B01L7/00 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6851 ,  B01L7/52 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2561/113 ,  C12Q2537/143 ,  C12Q2527/107

    摘要： The present invention provides a new protocol for quantifying multiplex real-time polymerase chain reaction (PCR). In particular, the present invention provides methods of quantifying multiple PCR products or amplicons in a single real-time PCR reaction based on the different melting temperatures (Tm) of each amplicon and the emission changes of double stranded DNA dyes such as SYBR Green I when amplicons are in duplex or in separation. For a specific amplicon with a Tm, the emission difference between the emission reading taken at a temperature below the Tm and the emission reading taken at a temperature above the Tm corresponds to the emission value of the amplicon in duplex. Accordingly, the emission difference of each amplicon in a single PCR reaction can be used to quantify each amplicon. The present invention further provides computer programs or computer products which perform the methods described herein.

    摘要翻译： 本发明提供了一种用于量化多重实时聚合酶链反应（PCR）的新方案。 特别地，本发明提供了基于每个扩增子的不同解链温度（Tm）和双链DNA染料如SYBR Green I的发射变化在单次实时PCR反应中定量多个PCR产物或扩增子的方法， 扩增子是双链体或分离的。 对于具有Tm的特异性扩增子，在低于Tm的温度下获取的发射读数与在高于Tm的温度下获得的发射读数之间的发射差异对应于双链体扩增子的发射值。 因此，单个PCR反应中每个扩增子的发射差异可用于量化每个扩增子。 本发明还提供了执行本文描述的方法的计算机程序或计算机产品。