公开(公告)号：US20160215315A1

公开(公告)日：2016-07-28

申请号：US14914520

申请日：2014-09-05

申请人： Glycom A/S

发明人： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC分类号： C12P19/18 ,  C12P19/26

CPC分类号： C12P19/18 ,  C07H3/06 ,  C08B37/006 ,  C12P19/00 ,  C12P19/26 ,  C12Y204/01065 ,  C12Y204/01087 ,  C12Y204/01099

摘要： The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

摘要翻译： 本申请公开了一种生产至少四个单糖单元的寡糖的方法，有利地是HMO，特别是仅四个单糖单元的HMO，所述方法包括以下步骤：在含有岩藻糖基化的唾液酸化或N- 乙酰基 - 葡糖胺化的乳糖三糖作为受体，一种遗传修饰的细胞，其具有编码能够修饰至少四个单糖单元的寡糖的生物合成途径中的所述受体或必需中间体的酶的重组基因，有利地是HMO，特别是 来自所述受体的仅四种单糖单元的HMO。

公开(公告)号：US20160208302A1

公开(公告)日：2016-07-21

申请号：US14915421

申请日：2014-09-05

申请人： GLYCOM A/S

发明人： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC分类号： C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04

CPC分类号： C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

摘要： The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

摘要翻译： 本申请公开了通过用乳糖培养具有编码单个岩藻糖基转移酶的重组基因的遗传修饰的细胞，以高产率制备2'-FL和DFL的混合物的方法。 所得到的2'-FL和DFL的混合物可以进行由酸引发的水解或由岩藻糖苷酶介导的产生高产率岩藻糖的水解。