公开(公告)号：US20160215315A1

公开(公告)日：2016-07-28

申请号：US14914520

申请日：2014-09-05

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C12P19/26

CPC classification number: C12P19/18 ,  C07H3/06 ,  C08B37/006 ,  C12P19/00 ,  C12P19/26 ,  C12Y204/01065 ,  C12Y204/01087 ,  C12Y204/01099

Abstract: The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

Abstract translation: 本申请公开了一种生产至少四个单糖单元的寡糖的方法，有利地是HMO，特别是仅四个单糖单元的HMO，所述方法包括以下步骤：在含有岩藻糖基化的唾液酸化或N- 乙酰基 - 葡糖胺化的乳糖三糖作为受体，一种遗传修饰的细胞，其具有编码能够修饰至少四个单糖单元的寡糖的生物合成途径中的所述受体或必需中间体的酶的重组基因，有利地是HMO，特别是 来自所述受体的仅四种单糖单元的HMO。

公开(公告)号：US20160208302A1

公开(公告)日：2016-07-21

申请号：US14915421

申请日：2014-09-05

Applicant: GLYCOM A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04

CPC classification number: C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

Abstract: The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

Abstract translation: 本申请公开了通过用乳糖培养具有编码单个岩藻糖基转移酶的重组基因的遗传修饰的细胞，以高产率制备2'-FL和DFL的混合物的方法。 所得到的2'-FL和DFL的混合物可以进行由酸引发的水解或由岩藻糖苷酶介导的产生高产率岩藻糖的水解。

公开(公告)号：US09902984B2

公开(公告)日：2018-02-27

申请号：US14915421

申请日：2014-09-05

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04 ,  C12P19/00 ,  C07H1/00

CPC classification number: C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

Abstract: The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

公开(公告)号：US20140303363A1

公开(公告)日：2014-10-09

申请号：US14363675

申请日：2012-12-07

Applicant: Glycom A/S

Inventor： Markus Hederos ,  Christian Risinger ,  Julien Boutet ,  Gyula Dekany

IPC: C07H15/18 ,  C07H15/12 ,  C07H5/06

CPC classification number: C07H15/12 ,  C07H1/00 ,  C07H5/06 ,  C07H15/18 ,  Y02P20/55

Abstract: The invention relates to providing isolactosamine (Galβ1-3GlcNH2, formula 1) and salts thereof in the form of either anomer or mixture thereof, as well as hydrates or solvates of the free base and salts thereof. The synthesis of isolactosamine and its use in the synthesis of lacto-N-biose containing oligosaccharides are also disclosed.

Abstract translation: 本发明涉及提供异构体或其混合物形式的异乳糖胺（Gal＆bgr; 1-3GlcNH 2，式1）及其盐，以及游离碱及其盐的水合物或溶剂合物。 还公开了异乳糖胺的合成及其在合成含有乳-N-降糖的低聚糖中的用途。

公开(公告)号：US20160289721A1

公开(公告)日：2016-10-06

申请号：US15161887

申请日：2016-05-23

Applicant: GLYCOM A/S

Inventor： Gyula DEKANY ,  Elise Champion ,  Markus Hederos

IPC: C12P19/14 ,  C12P19/12 ,  C12P19/04

CPC classification number: C12P19/14 ,  C07H1/00 ,  C07H5/04 ,  C07H13/04 ,  C12P19/04 ,  C12P19/12

Abstract: A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b).

Abstract translation: 人乳寡糖（HMO）或其前体多样化的方法，可通过该方法获得的化合物以及涉及这些化合物的用途和组合物。 该方法包括：a）提供至少一种化合物或选自下组的化合物或混合物：选自唾液酸化的和/或通式2的岩藻糖化的乳糖衍生物及其盐; b）向所述至少一种化合物或根据步骤a）提供的化合物的混合物中加入至少一种包含转糖苷酶活性的酶; 和c）孵育根据步骤b）获得的混合物。

公开(公告)号：US20150133647A1

公开(公告)日：2015-05-14

申请号：US14406379

申请日：2013-06-07

Applicant: Glycom A/S

Inventor： Pauline Peltier-Pain ,  Gyula Dekany ,  Rémy Dureau ,  Christian Risinger ,  Markus Hederos ,  Elise Champion

IPC: C12P19/44 ,  C12P19/46

CPC classification number: C12P19/44 ,  C12P19/28 ,  C12P19/64

Abstract: The application discloses a method for producing anomerically protected glycosidic oligosaccharide derivatives comprising the step of culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor. The application further discloses a method for producing an oligosaccharide comprising the steps of: (a) culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor to produce an anomerically protected glycosidic oligosaccharide derivative, then (b) removing/deprotecting the anomeric protective group.

Abstract translation: 本申请公开了一种用于制备异头保护的糖苷寡糖衍生物的方法，其包括在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞的步骤 活化的糖核苷酸与所述乳糖受体。 本申请进一步公开了一种生产寡糖的方法，包括以下步骤：（a）在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞 的活性糖核苷酸与所述乳糖受体产生异头保护的糖苷寡糖衍生物，然后（b）去除/去保护端基异构保护基团。

公开(公告)号：US20140235850A1

公开(公告)日：2014-08-21

申请号：US14348334

申请日：2012-10-01

Applicant: Glycom A/S

Inventor： Imre Kovács ,  István Bajza ,  Markus Hederos ,  Gyula Dekany ,  Sándor Demkó ,  Nikolay Khanzhin

IPC: C07H1/00 ,  C07H3/04 ,  C07H3/06

CPC classification number: C07H1/00 ,  A23L29/30 ,  C07H3/04 ,  C07H3/06 ,  C07H15/18

Abstract: The invention relates to a method for making precursors of HMO core structures comprising a step of reacting an N-acetyllactosamine or lacto-N-biose derivative donor with a lactose or N-acetyllactosamine derivative acceptor, wherein the donor is an oxazoline donor.

Abstract translation: 本发明涉及一种制备HMO核心结构的前体的方法，包括使N-乙酰基乳糖胺或乳-N-降糖衍生物供体与乳糖或N-乙酰基乳糖胺衍生物受体反应的步骤，其中供体是恶唑啉供体。

公开(公告)号：US20140228554A1

公开(公告)日：2014-08-14

申请号：US14005796

申请日：2012-03-19

Applicant: Glycom A/S

Inventor： Elise Champion ,  Gyula Dekany ,  Markus Hederos ,  Karoly Agoston

IPC: C12P19/60 ,  C07H15/203 ,  C07H15/18 ,  C07H17/04

CPC classification number: C12P19/60 ,  C07H15/08 ,  C07H15/18 ,  C07H15/203 ,  C07H17/04 ,  Y02P20/55

Abstract: A method for the synthesis of a fucooligosaccharide glycosides by reacting a fucosyl donor with H-A-R1 or a salt thereof, wherein A and R1 are as defined herein, under the catalysis of an enzyme capable of transferring fucose is provided. The fucooligosaccharid glycoside compounds, or derivatives thereof, their use in the manufacture of human milk oligosaccharides, and a method of manufacture of human milk oligosaccharides, are also provided.

Abstract translation: 提供了通过使岩藻糖基供体与H-A-R1或其盐（其中A和R 1如本文所定义）在能够转移岩藻糖的酶的催化下反应来合成软脂寡糖糖苷的方法。 还提供了岩藻糖糖苷化合物或其衍生物，其在制造人乳低聚糖中的用途，以及制造人乳低聚糖的方法。