公开(公告)号：US09902984B2

公开(公告)日：2018-02-27

申请号：US14915421

申请日：2014-09-05

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04 ,  C12P19/00 ,  C07H1/00

CPC classification number: C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

Abstract: The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

公开(公告)号：US20150183816A1

公开(公告)日：2015-07-02

申请号：US14408877

申请日：2013-06-24

Applicant: GLYCOM A/S

Inventor： Elise Champion ,  Dóra Molnár-Gábor ,  Gyula Dekany

IPC: C07H3/06 ,  A23L1/30 ,  A23L1/29 ,  C12P19/04 ,  C12P19/18

CPC classification number: C07H3/06 ,  A23L5/00 ,  A23L29/30 ,  A23L33/10 ,  A23L33/40 ,  A23V2002/00 ,  C12P19/04 ,  C12P19/18

Abstract: The invention relates to a method for making modified galactooligosaccharides comprising the step of enzymatic glycosylation, the modified galactooligosaccharides obtainable by the method and consumable products containing them.

Abstract translation: 本发明涉及一种制备改性低聚半乳糖的方法，包括酶促糖基化步骤，可通过该方法获得的改良的低聚半乳糖和含有它们的消费品。

公开(公告)号：US20150182549A1

公开(公告)日：2015-07-02

申请号：US14408495

申请日：2013-06-24

Applicant: Glycom A/S

Inventor： Elise Champion ,  Dóra Molnár-Gábor ,  Gyula Dekany

IPC: A61K31/715 ,  A23L1/30 ,  A23L1/29

CPC classification number: A61K31/715 ,  A23L29/275 ,  A23L33/10 ,  A23L33/40 ,  A23V2002/00 ,  C07H1/00 ,  C07H9/06 ,  C07H13/04 ,  C12P19/00 ,  C12P19/18 ,  C12P19/26 ,  C12Y302/01038 ,  C12Y302/01051 ,  A23V2200/304 ,  A23V2200/3202 ,  A23V2200/324 ,  A23V2250/28

Abstract: A method for modifying galactosyl disaccharides in order to enhance their bifidogenic effect, comprising reacting at least one glycosyl donor with a precursor galactosyl disaccharide or a mixture of precursor galactosyl disaccharides using enzyme catalysis, compounds obtainable by said method and the use of such compounds in consumable products.

Abstract translation: 一种用于改善半乳糖二糖以增强其双发效应的方法，包括使用酶催化使至少一种糖基供体与前体半乳糖二糖或前体半乳糖二糖的混合物反应，可通过所述方法获得的化合物和这些化合物在消耗品中的用途 产品。

公开(公告)号：US10364449B2

公开(公告)日：2019-07-30

申请号：US14914520

申请日：2014-09-05

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Jondelius Hederos

IPC: C12P19/18 ,  C07H3/06 ,  C12P19/26 ,  C08B37/00 ,  C12P19/00

Abstract: The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

公开(公告)号：US20160215315A1

公开(公告)日：2016-07-28

申请号：US14914520

申请日：2014-09-05

Applicant: Glycom A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C12P19/26

CPC classification number: C12P19/18 ,  C07H3/06 ,  C08B37/006 ,  C12P19/00 ,  C12P19/26 ,  C12Y204/01065 ,  C12Y204/01087 ,  C12Y204/01099

Abstract: The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.

Abstract translation: 本申请公开了一种生产至少四个单糖单元的寡糖的方法，有利地是HMO，特别是仅四个单糖单元的HMO，所述方法包括以下步骤：在含有岩藻糖基化的唾液酸化或N- 乙酰基 - 葡糖胺化的乳糖三糖作为受体，一种遗传修饰的细胞，其具有编码能够修饰至少四个单糖单元的寡糖的生物合成途径中的所述受体或必需中间体的酶的重组基因，有利地是HMO，特别是 来自所述受体的仅四种单糖单元的HMO。

公开(公告)号：US20160208302A1

公开(公告)日：2016-07-21

申请号：US14915421

申请日：2014-09-05

Applicant: GLYCOM A/S

Inventor： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC: C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04

CPC classification number: C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

Abstract: The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

Abstract translation: 本申请公开了通过用乳糖培养具有编码单个岩藻糖基转移酶的重组基因的遗传修饰的细胞，以高产率制备2'-FL和DFL的混合物的方法。 所得到的2'-FL和DFL的混合物可以进行由酸引发的水解或由岩藻糖苷酶介导的产生高产率岩藻糖的水解。