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公开(公告)号：US09902984B2
公开(公告)日：2018-02-27
申请号：US14915421
申请日：2014-09-05
申请人： Glycom A/S
摘要： The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.
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公开(公告)号：US20220235388A1
公开(公告)日：2022-07-28
申请号：US17657787
申请日：2022-04-04
申请人： GLYCOM A/S
发明人： Margit Pedersen , Manos Papadakis , Peter Becker , Eric Samain , Pauline Peltier-Pain , Katrine Bych , Ted Johanson , Elise Champion , Gyula Dekany
摘要： The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.
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公开(公告)号：US09816122B2
公开(公告)日：2017-11-14
申请号：US14430930
申请日：2013-09-25
申请人： Glycom A/S
发明人： Pauline Peltier-Pain , Gyula Dekany , Christophe Pain , Pierre Chassagne , Nicolas Fierfort , Dóra Molnàr-Gàbor
摘要： The invention relates to a method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, using a genetically modified cell.
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公开(公告)号：US20150252400A1
公开(公告)日：2015-09-10
申请号：US14430930
申请日：2013-09-25
申请人： GLYCOM A/S
发明人： Pauline Peltier-Pain , Gyula Dekany , Christophe Pain , Pierre Chassagne , Nicolas Fierfort , Dóra Molnàr-Gàbor
摘要： The invention relates to a method for producing a glycoconjugate comprising an oligosaccharide part covalently linked to a non-sugar moiety selected from the group consisting of amino acids, peptides, proteins, lipids, longer alkyl groups, polyethylene glycols, α,β-unsaturated amido group and polyvinyl alcohols, using a genetically modified cell.
摘要翻译： 本发明涉及一种制备糖缀合物的方法，其包含与非糖部分共价连接的寡糖部分，所述寡糖部分选自氨基酸，肽，蛋白质，脂质，较长的烷基，聚乙二醇，α，β-不饱和 酰胺基和聚乙烯醇，使用转基因细胞。
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公开(公告)号：US20170175154A1
公开(公告)日：2017-06-22
申请号：US15321996
申请日：2015-06-29
申请人： GLYCOM A/S
发明人： Eric Samain , Pauline Peltier-Pain , Katrine Bych , Ted Johanson , Elise Champion , Gyula Dekany
CPC分类号： C12P19/18 , C07K14/245 , C12N9/1029 , C12N9/1051 , C12N9/1205 , C12N9/2402 , C12N9/2471 , C12N15/52 , C12Y203/01018 , C12Y204/01149 , C12Y302/01023 , C12Y302/01026
摘要： The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.
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6.发明申请Method for Producing Oligosaccharides and Oligosaccharide Glycosides by Fermentation 审中-公开通过发酵生产低聚糖和寡糖糖苷的方法公开(公告)号：US20150133647A1
公开(公告)日：2015-05-14
申请号：US14406379
申请日：2013-06-07
申请人： Glycom A/S
发明人： Pauline Peltier-Pain , Gyula Dekany , Rémy Dureau , Christian Risinger , Markus Hederos , Elise Champion
摘要： The application discloses a method for producing anomerically protected glycosidic oligosaccharide derivatives comprising the step of culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor. The application further discloses a method for producing an oligosaccharide comprising the steps of: (a) culturing, in a culture medium containing an anomerically protected lactose acceptor, a genetically modified cell having a recombinant gene that encodes a glycosyl transferase that can transfer a glycosyl residue of an activated sugar nucleotide to said lactose acceptor to produce an anomerically protected glycosidic oligosaccharide derivative, then (b) removing/deprotecting the anomeric protective group.
摘要翻译： 本申请公开了一种用于制备异头保护的糖苷寡糖衍生物的方法，其包括在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞的步骤 活化的糖核苷酸与所述乳糖受体。 本申请进一步公开了一种生产寡糖的方法，包括以下步骤：（a）在含有异头保护的乳糖受体的培养基中培养具有编码可转移糖基残基的糖基转移酶的重组基因的遗传修饰细胞 的活性糖核苷酸与所述乳糖受体产生异头保护的糖苷寡糖衍生物，然后（b）去除/去保护端基异构保护基团。
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公开(公告)号：US11293042B2
公开(公告)日：2022-04-05
申请号：US16927700
申请日：2020-07-13
申请人： GLYCOM A/S
发明人： Margit Pedersen , Manos Papadakis , Peter Becker , Eric Samain , Pauline Peltier-Pain , Katrine Bych , Ted Johanson , Elise Champion , Gyula Dekany
摘要： The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.
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公开(公告)号：US10731193B2
公开(公告)日：2020-08-04
申请号：US15321996
申请日：2015-06-29
申请人： GLYCOM A/S
发明人： Eric Samain , Pauline Peltier-Pain , Katrine Bych , Ted Johanson , Elise Champion , Gyula Dekany
摘要： The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.
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公开(公告)号：US10364449B2
公开(公告)日：2019-07-30
申请号：US14914520
申请日：2014-09-05
申请人： Glycom A/S
摘要： The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.
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公开(公告)号：US20160215315A1
公开(公告)日：2016-07-28
申请号：US14914520
申请日：2014-09-05
申请人： Glycom A/S
CPC分类号： C12P19/18 , C07H3/06 , C08B37/006 , C12P19/00 , C12P19/26 , C12Y204/01065 , C12Y204/01087 , C12Y204/01099
摘要： The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.
摘要翻译： 本申请公开了一种生产至少四个单糖单元的寡糖的方法，有利地是HMO，特别是仅四个单糖单元的HMO，所述方法包括以下步骤：在含有岩藻糖基化的唾液酸化或N- 乙酰基 - 葡糖胺化的乳糖三糖作为受体，一种遗传修饰的细胞，其具有编码能够修饰至少四个单糖单元的寡糖的生物合成途径中的所述受体或必需中间体的酶的重组基因，有利地是HMO，特别是 来自所述受体的仅四种单糖单元的HMO。
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