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    METHOD OF POOLING BLOOD SAMPLES
    13.
    发明申请
    METHOD OF POOLING BLOOD SAMPLES 有权

    公开(公告)号：US20220221382A1

    公开(公告)日：2022-07-14

    申请号：US17652172

    申请日：2022-02-23

    Applicant: Gen-Probe Incorporated

    Inventor： Jeffrey M. LINNEN ,  Jijumon CHELLISERRY

    IPC: G01N1/38 ,  C12Q1/6806 ,  C12Q1/04 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/6893 ,  C12Q1/70

    Abstract: A method for detecting the presence of a pathogen in a whole blood sample aliquot, includes providing a sample aliquot from each of a number of whole blood samples, thereby providing a plurality of whole blood sample aliquots, separately contacting each of the whole blood sample aliquots of the plurality of whole blood sample aliquots with a lysis reagent, whereby at least a portion of the blood cells in each of the whole blood sample aliquots lyse, pooling the lysed whole blood sample aliquots to form a pooled lysate, and testing the pooled lysate for presence of a pathogen. Identification of the presence of the pathogen in the pooled lysate indicates the presence of the pathogen in at least one of the whole blood sample aliquots.

    METHOD OF POOLING BLOOD SAMPLES
    15.
    发明申请
    METHOD OF POOLING BLOOD SAMPLES 审中-公开

    公开(公告)号：US20180143115A1

    公开(公告)日：2018-05-24

    申请号：US15573014

    申请日：2016-05-12

    Applicant: GEN-PROBE INCORPORATED

    Inventor： Jeffrey M. LINNEN ,  Jijumon CHELLISERRY

    IPC: G01N1/38 ,  C12Q1/6893 ,  C12Q1/6806 ,  C12Q1/70

    CPC classification number: G01N1/38 ,  C12N1/06 ,  C12N15/1003 ,  C12Q1/04 ,  C12Q1/6806 ,  C12Q1/6893 ,  C12Q1/70

    Abstract: The disclosure provides methods of pooled analysis of samples containing red blood cells, such as whole blood cell samples. The methods are particularly useful for screening whole blood samples collected from many individuals to be used for transfusions and the like. Aliquots of such samples are individually contacted with a lysis reagent, further described below, before pooling to lyse red blood cells in the samples. After pooling, the lysates, the combined lysate is tested for presence of target(s) characteristic of pathogen(s). The method are particularly useful for identifying pathogens of red blood cells, but can also be used to nm a complete panel of testing including for all of the typical targets.

    RED BLOOD CELL LYSIS SOLUTION
    17.
    发明申请
    RED BLOOD CELL LYSIS SOLUTION 审中-公开
    红血球细胞溶解度

    公开(公告)号：US20160201144A1

    公开(公告)日：2016-07-14

    申请号：US14918131

    申请日：2015-10-20

    Applicant: Gen-Probe Incorporated

    Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

    IPC: C12Q1/68

    CPC classification number: C12Q1/6893 ,  C12N1/06 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2527/125

    Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

    Abstract translation: 本发明提供了用于裂解红细胞的裂解试剂，从而以适合于分析的形式从寄生生物体释放靶，例如RNA。 试剂至少包括氯化铵和阴离子洗涤剂，并且可以包括抗凝剂。 该试剂用于裂解红细胞，保护释放的靶免受裂解物中的降解，并且与目标捕获，扩增，检测或测序等目标分析的后续步骤兼容。

    DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE
    18.
    发明公开
    DETECTING BABESIA SPECIES NUCLEIC ACID IN A SAMPLE 审中-公开

    公开(公告)号：US20230227922A1

    公开(公告)日：2023-07-20

    申请号：US18159218

    申请日：2023-01-25

    Applicant: Gen-Probe Incorporated

    Inventor： Vanessa BRES ,  Deanna SELF ,  Jeffrey M. LINNEN

    IPC: C12Q1/6893 ,  C12Q1/6806

    CPC classification number: C12Q1/6893 ,  C12Q1/6806 ,  C12Q2600/16 ,  C12Q2600/166

    Abstract: There is described herein a method for specifically detecting Babesia species nucleic acid in a sample, which in one aspect comprises: (1) contacting a sample, said sample suspected of containing Babesia species nucleic acid, with at least two oligomers for amplifying a target region of a Babesia species target nucleic acid, wherein the at least two amplification oligomers comprise: (a) a first amplification oligomer comprising a first target-hybridizing sequence (i) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:66 and comprises SEQ ID NO:56 or 57; or (ii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:96 and comprises SEQ ID NO:101; or (iii) that is from about 15 to about 33 contiguous nucleotides in length, is contained in the sequence of SEQ ID NO:97 and comprises SEQ ID NO:101; (iv) comprises or consists of SEQ ID NO:8; (v) comprises or consists of SEQ ID NO:83 and (b) a second amplification oligomer comprising a second target-hybridizing sequence that is from about 15 to about 33 contiguous nucleotides in length, and (i) is contained in SEQ ID NO:68 and comprises SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, or SEQ ID NO:85; or (ii) is contained in SEQ ID NO:67 and comprises SEQ ID NO:45 or SEQ ID NO:52; or (iii) is contained in SEQ ID NO:70 and comprises SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, or SEQ ID NO:51; (2) performing an in vitro nucleic acid amplification reaction, wherein any Babesia target nucleic acid present in said sample is used as a template for generating an amplification product; and (3) detecting the presence or absence of the amplification product, thereby indicating the presence or absence of Babesia species target nucleic acid in said sample.

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