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公开(公告)号：US20230365704A1
公开(公告)日：2023-11-16
申请号：US18310448
申请日：2023-05-01
Applicant: Genentech, Inc.
Inventor： Yung-Hsiang KAO , Michael W. LAIRD , Melody Trexler SCHMIDT , Rita L. WONG , Daniel P. HEWITT
IPC: C07K16/28 , C07K1/14 , A61K39/395
CPC classification number: C07K16/2887 , C07K1/14 , A61K39/39591 , C07K2317/24
Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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公开(公告)号：US20230069966A1
公开(公告)日：2023-03-09
申请号：US17864255
申请日：2022-07-13
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20210171997A1
公开(公告)日：2021-06-10
申请号：US17067059
申请日：2020-10-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20170313780A1
公开(公告)日：2017-11-02
申请号：US15488917
申请日：2017-04-17
Applicant: Genentech, Inc.
Inventor： Yung-Hsiang KAO , Michael W. LAIRD , Melody Trexler SCHMIDT , Rita L. WONG , Daniel P. HEWITT
IPC: C07K16/28 , A61K39/395 , C07K1/14
CPC classification number: C07K16/2887 , A61K39/39591 , C07K1/14 , C07K2317/24
Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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公开(公告)号：US20160130624A1
公开(公告)日：2016-05-12
申请号：US14734848
申请日：2015-06-09
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
Abstract translation: 本发明涉及生产重组蛋白的方法，其包括（a）发酵原核宿主细胞，其中所述原核宿主细胞已经用编码所述重组蛋白的核酸转化，和（b）在dO2水平的条件下收获所述重组蛋白 大于0％，和（c）将所述重组蛋白纯化至过滤的体积，其中所述过滤的体积不含可检测的DHNA重组蛋白加合物，如通过IEC测定在310nm测量的。 本发明还涉及一种生产重组蛋白的方法，其包括（a）发酵menE基因缺失的原核宿主细胞，其中所述原核宿主细胞已用编码所述重组蛋白的核酸转化，（b）收获所述重组蛋白; 和（c）将所述重组蛋白纯化至过滤体积，其中所述过滤的体积不含可检测的DHNA重组蛋白质加合物，如通过IEC测定在310nm测量的，并且其中重组蛋白质产量增加约20％或 更大
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Patent Agency Ranking
公开(公告)号：US20240425610A1
公开(公告)日：2024-12-26
申请号：US18824681
申请日：2024-09-04
Applicant: Genentech, Inc.
Inventor： Yung-Hsiang KAO , Michael W. LAIRD , Melody Trexler SCHMIDT , Rita L. WONG , Daniel P. HEWITT
IPC: C07K16/28 , A61K39/395 , C07K1/14
Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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公开(公告)号：US20240158825A1
公开(公告)日：2024-05-16
申请号：US18419368
申请日：2024-01-22
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Richard ST. JOHN , Jane V. GUNSON , Kimberly KALEAS , Deepa NADARAJAH , Rachel L.E. ADAMS , Bradley R. SNEDECOR
Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm. The present invention also contemplates a method of producing a recombinant protein comprising (a) fermenting a menE gene-deleted prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, (b) harvesting said recombinant protein; and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm, and wherein the recombinant protein yield is increased by about 20% or greater.
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公开(公告)号：US20240158527A1
公开(公告)日：2024-05-16
申请号：US18410949
申请日：2024-01-11
Applicant: Genentech, Inc.
Inventor： Yung-Hsiang KAO , Michael W. LAIRD , Melody Trexler SCHMIDT , Rita L. WONG , Daniel P. HEWITT
IPC: C07K16/28 , A61K39/395 , C07K1/14
CPC classification number: C07K16/2887 , A61K39/39591 , C07K1/14 , C07K2317/24
Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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公开(公告)号：US20200317801A1
公开(公告)日：2020-10-08
申请号：US16847309
申请日：2020-04-13
Applicant: Genentech, Inc.
Inventor： Yung-Hsiang KAO , Michael W. LAIRD , Melody Trexler SCHMIDT , Rita L. WONG , Daniel P. HEWITT
IPC: C07K16/28 , A61K39/395 , C07K1/14
Abstract: The invention concerns methods and means for preventing the reduction of disulfide bonds during the recombinant production of disulfide-containing polypeptides. In particular, the invention concerns the prevention of disulfide bond reduction during harvesting of disulfide-containing polypeptides, including antibodies, from recombinant host cell cultures.
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公开(公告)号：US20200056218A1
公开(公告)日：2020-02-20
申请号：US16536149
申请日：2019-08-08
Applicant: Genentech, Inc.
Inventor： Michael W. LAIRD , Karthik VEERAVALLI
IPC: C12P21/00 , C07K16/32 , C07K16/28 , C12N15/70 , C12N9/10 , C07K16/40 , C07K16/18 , C07K16/22 , C12P21/02
Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.
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