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11.发明授权Apparatus and method for high spatial resolution imaging of a structure of a sample 有权用于样品结构的高空间分辨率成像的装置和方法公开(公告)号：US08362448B2
公开(公告)日：2013-01-29
申请号：US12867291
申请日：2009-02-03
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译： 用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度 体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。 提供用于发射切换辐射并用于发射激发辐射的至少一个光源。 至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。 切换是染料分子的光活化或光激活。 提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。
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公开(公告)号：US08350230B2
公开(公告)日：2013-01-08
申请号：US12680265
申请日：2008-09-18
IPC分类号： G01N21/64
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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公开(公告)号：US08213081B2
公开(公告)日：2012-07-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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公开(公告)号：US20110031414A1
公开(公告)日：2011-02-10
申请号：US12937103
申请日：2009-04-04
IPC分类号： G01J1/58
CPC分类号： G02B21/16 , G02B21/002 , G02B21/06 , G02B21/10
摘要： A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
摘要翻译： 提供了特别用于成像荧光寿命显微术方法的显微镜装置。 显微镜装置包括用于产生照明光束的照明装置，用于空间分辨地获取由待检查对象发射的发射辐射的成像检测器，照明装置和待检查对象之间的照射光束路径，以及检测 要检查的物体与检测器之间的光束路径。 照明光束路径包括照明光学器件，其设计成产生横向于照明光束路径的轴线延伸的照明辐射的光片，其中检测光束路径的轴线基本上垂直于光片的截面定向， 的被检查对象。 照明装置包括脉冲激光器。
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公开(公告)号：US20100309548A1
公开(公告)日：2010-12-09
申请号：US12680265
申请日：2008-09-18
IPC分类号： G02B21/06
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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公开(公告)号：US20100134881A1
公开(公告)日：2010-06-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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公开(公告)号：US08921809B2
公开(公告)日：2014-12-30
申请号：US12937103
申请日：2009-04-04
CPC分类号： G02B21/16 , G02B21/002 , G02B21/06 , G02B21/10
摘要： A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
摘要翻译： 提供了特别用于成像荧光寿命显微术方法的显微镜装置。 显微镜装置包括用于产生照明光束的照明装置，用于空间分辨地获取由待检查对象发射的发射辐射的成像检测器，照明装置和待检查对象之间的照明光束路径，以及检测 要检查的物体与检测器之间的光束路径。 照明光束路径包括照明光学器件，其设计成产生横向于照明光束路径的轴线延伸的照明辐射的光片，其中检测光束路径的轴线基本上垂直于光片的截面定向， 的被检查对象。 照明装置包括脉冲激光器。
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公开(公告)号：US08547634B2
公开(公告)日：2013-10-01
申请号：US12680165
申请日：2008-09-16
IPC分类号： G02B21/06
CPC分类号： G02B21/32 , G02B21/0032
摘要： The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample.
摘要翻译： 本发明涉及用于光调制样品的光学装置，其包括用于接收样品的样品保持器，包括照明光源的照明装置和用于用光照射样品的照明光束路径。 它还包括检测装置，用于检测从样本辐射的光和成像光学元件，其通过成像光束路径中的成像目标将检测装置上的样品成像在检测装置上，其中光片在 成像目标，并且其中所述成像物镜具有与所述光片的平面以不同于零的角度相交的光轴。 此外，该装置还具有用于对样品进行光电处理的装置。
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19.发明申请公开(公告)号：US20120282667A1
公开(公告)日：2012-11-08
申请号：US13554232
申请日：2012-07-20
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices arc proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了一种方法和装置，其中相对于检测对象的光轴的样本的取向可以重复地改变，并且在这样做时，保持样本，使得从每个检测方向确保样本的基本上无障碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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20.发明申请DEVICE FOR HOLDING AND POSITIONING A SAMPLE RELATIVE TO A MICROSCOPE LENS 审中-公开用于保存和定位相对于微镜镜头的样品的装置公开(公告)号：US20110170182A1
公开(公告)日：2011-07-14
申请号：US13056417
申请日：2009-07-28
IPC分类号： G02B21/26
CPC分类号： G02B21/26 , G02B21/0088 , G02B21/33 , G02B21/34
摘要： An arrangement for holding and positioning a sample in the detection area of the objective of a microscope, the detection area being located in a chamber which is filled with an immersion liquid. This arrangement includes (1) a sample holder to which the sample is affixed so as to lie upon a point P in a coordinate system X, Y, Z, coordinate Z being defined by the optical axis of the microscope objective and the coordinate origin laying within the detection area, (2) a device by which the position of point P can be varied within the coordinate system X, Y, Z, wherein the range of variation comprehends the detection area, and (3) a device for rotating the sample affixed to the sample holder around point P, wherein a straight line G which encloses with coordinate Z an angle α whose size can be changed is the axis of rotation.
摘要翻译： 用于将样品保持和定位在显微镜的目标的检测区域中的装置，检测区域位于填充有浸没液体的室中。 这种布置包括（1）样品固定器，样品被固定在其上，以便位于坐标系X，Y，Z中的点P上，坐标Z由显微镜物镜的光轴和坐标原点铺设 在检测区域内，（2）能够在坐标系X，Y，Z内改变点P的位置的装置，其中变化范围包括检测区域，以及（3）旋转样品的装置 固定在点P附近的样品架上，其中以坐标Z包围尺寸可以改变的角度α的直线G是旋转轴。
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