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公开(公告)号：US08350230B2
公开(公告)日：2013-01-08
申请号：US12680265
申请日：2008-09-18
IPC分类号： G01N21/64
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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公开(公告)号：US20100309548A1
公开(公告)日：2010-12-09
申请号：US12680265
申请日：2008-09-18
IPC分类号： G02B21/06
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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公开(公告)号：US20100177381A1
公开(公告)日：2010-07-15
申请号：US12597144
申请日：2008-04-04
IPC分类号： G02B21/34
CPC分类号： G02B21/26
摘要： The invention relates to a sample holding system for a microscope, comprising a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. The sample holding system also comprises means for the translatory movement of the sample in relation to a detection objective of the microscope, and means for rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective. The means for rotating the sample in such a sample holding system comprise a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber, said drives transmitting the rotational movement to the holder, or the rotational movement is generated directly by the movement of the entire sample chamber.
摘要翻译： 本发明涉及一种用于显微镜的样品保持系统，其包括具有上部开口并填充有浸没液体的样品室，并且将嵌入在透明嵌入物中的样品放置在保持器中。 样品保持系统还包括用于相对于显微镜的检测目标的样品的平移运动的装置，以及用于使样品围绕基本上垂直的旋转轴旋转的装置，该平面形成与零的光轴不同的角度 检测目标。 在这种样品保持系统中旋转样品的装置包括旋转驱动器，其设置有设置在样品室上方的磁耦合或带驱动和/或齿轮旋转驱动器，所述驱动器将旋转运动传递到保持器，或 通过整个样品室的运动直接产生旋转运动。
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公开(公告)号：US08228499B2
公开(公告)日：2012-07-24
申请号：US12682234
申请日：2008-09-18
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了相对于检测对象的光轴的试样的取向可以反复改变的方法和装置，并且在这样做的同时，保持试样，使得从每个检测方向确保了样本的基本无阻碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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公开(公告)号：US08699133B2
公开(公告)日：2014-04-15
申请号：US12597144
申请日：2008-04-04
IPC分类号： G02B21/26
CPC分类号： G02B21/26
摘要： A sample holding system for a microscope, including a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. Translatory movement of the sample in relation to a detection objective of the microscope, and rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective is included. Rotating the sample includes a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber.
摘要翻译： 一种用于显微镜的样品保持系统，包括具有上部开口并填充有浸没液体的样品室，并且将嵌入在透明嵌入物中的样品放置在保持器中。 样品相对于显微镜的检测目标的平移运动，并且包括在与检测对象的光轴形成不同于零的角度的平面中基本上垂直的旋转轴线旋转样品。 旋转样品包括旋转驱动器，其设置有设置在样品室上方的磁耦合或带驱动和/或齿轮旋转驱动。
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公开(公告)号：US20100239138A1
公开(公告)日：2010-09-23
申请号：US12682234
申请日：2008-09-18
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了相对于检测对象的光轴的试样的取向可以反复改变的方法和装置，并且在这样做的同时，保持试样，使得从每个检测方向确保了样本的基本无阻碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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公开(公告)号：US20100193673A1
公开(公告)日：2010-08-05
申请号：US12680165
申请日：2008-09-16
IPC分类号： H05H3/00
CPC分类号： G02B21/32 , G02B21/0032
摘要： The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample.
摘要翻译： 本发明涉及用于光调制样品的光学装置，其包括用于接收样品的样品保持器，包括照明光源的照明装置和用于用光照射样品的照明光束路径。 它还包括检测装置，用于检测从样本辐射的光和成像光学元件，其通过成像光束路径中的成像目标将检测装置上的样品成像在检测装置上，其中光片在 成像目标，并且其中所述成像物镜具有与所述光片的平面以不同于零的角度相交的光轴。 此外，该装置还具有用于对样品进行光电处理的装置。
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公开(公告)号：US08482854B2
公开(公告)日：2013-07-09
申请号：US12529051
申请日：2008-03-26
摘要： The invention is directed to a sample holder for a microscope. The sample holder comprises a sample chamber which is filled with an immersion liquid and in which a sample is located. The sample chamber has an upper opening. It further comprises means for translating the sample relative to a detection objective of the microscope, and means for rotating the sample around an axis of rotation extending in a substantially horizontal plane which encloses an angle other than zero degrees with the optical axis of the detection objective. In a sample holder of this kind, the sample is embedded in a transparent embedding medium having at least partially a greater solidity than the immersion liquid. Further, the sample chamber has means for horizontally supporting the embedded sample against the effect of gravity.
摘要翻译： 本发明涉及用于显微镜的样品架。 样品架包括样品室，其中填充有浸没液体并且样品位于样品室中。 样品室具有上部开口。 它还包括用于相对于显微镜的检测对象平移样本的装置，以及用于围绕围绕旋转轴旋转的装置的装置，该旋转轴在基本上水平的平面中旋转，该基本上水平的平面与检测目标的光轴 。 在这种样品保持器中，将样品嵌入至少部分具有比浸没液更大的固体的透明嵌入介质中。 此外，样品室具有用于水平地支撑嵌入样品免受重力影响的装置。
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9.发明授权Apparatus and method for high spatial resolution imaging of a structure of a sample 有权用于样品结构的高空间分辨率成像的装置和方法公开(公告)号：US08362448B2
公开(公告)日：2013-01-29
申请号：US12867291
申请日：2009-02-03
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译： 用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度 体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。 提供用于发射切换辐射并用于发射激发辐射的至少一个光源。 至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。 切换是染料分子的光活化或光激活。 提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。
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公开(公告)号：US08213081B2
公开(公告)日：2012-07-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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