公开(公告)号：US11649498B2

公开(公告)日：2023-05-16

申请号：US17720187

申请日：2022-04-13

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

CPC classification number: C12Q1/6874 ,  C12Q1/6813 ,  C12Q2565/514 ,  C12Q2600/156

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20220243269A1

公开(公告)日：2022-08-04

申请号：US17720187

申请日：2022-04-13

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US08765419B2

公开(公告)日：2014-07-01

申请号：US13872812

申请日：2013-04-29

Applicant: Illumina, Inc.

Inventor： Bernard Hirschbein ,  Filiz Gorpe-Yasar

IPC: C12Q1/66 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/66 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2565/301

Abstract: The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed herein are methods and systems which utilize improved pyrophosphate detection for nucleotide sequencing.

Abstract translation: 本技术涉及用于检测焦磷酸盐的方法和系统。 因此，本文公开了允许改进焦磷酸盐检测的方法和系统。 本文还公开了利用改进的焦磷酸盐检测用于核苷酸测序的方法和系统。

公开(公告)号：US20130237434A1

公开(公告)日：2013-09-12

申请号：US13872812

申请日：2013-04-29

Applicant: ILLUMINA, INC.

Inventor： Bernard Hirschbein ,  Filiz Gorpe-Yasar

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/66 ,  C12Q1/6869 ,  C12Q2565/607 ,  C12Q2565/301

Abstract: The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed herein are methods and systems which utilize improved pyrophosphate detection for nucleotide sequencing.

Abstract translation: 本技术涉及用于检测焦磷酸盐的方法和系统。 因此，本文公开了允许改进焦磷酸盐检测的方法和系统。 本文还公开了利用改进的焦磷酸盐检测用于核苷酸测序的方法和系统。