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    Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
    1.
    发明授权
    Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells 有权

    公开(公告)号：US11352668B2

    公开(公告)日：2022-06-07

    申请号：US16626115

    申请日：2018-07-31

    Applicant: ILLUMINA, INC.

    Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

    IPC: C12Q1/6874 ,  C12Q1/6813

    Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME
    2.
    发明申请
    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME 审中-公开
    一体化的微流控盒及其制造和使用方法

    公开(公告)号：US20170016060A1

    公开(公告)日：2017-01-19

    申请号：US15125124

    申请日：2015-03-11

    Applicant: ILLUMINA, INC.

    Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

    IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00

    Abstract: The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

    Abstract translation: 所公开的实施例涉及用于检测生物反应的微流体盒。 在一些实施方案中，微流控盒被配置为对核酸样品进行测序操作。 在一个方面，微流控筒包括限定用于处理要测序的核酸样品的通道和阀的流体层叠层，以及集成在堆中的固态CMOS生物传感器。 生物传感器具有被配置为检测生物反应的信号的有源区域，其中在操作期间基本上所有的有效区域可用于试剂递送和照明。 在另一方面，微流控盒包括：（a）流动池，包括包含一个或多个反应位点的反应位点区域; （b）用于将反应物输送到和/或从反应部位区域除去反应物的流体通道; （c）生物传感器，其具有被配置为检测反应部位区域中的生物反应信号的有源区域。 反应部位区域靠近生物传感器的有效区域，反应部位区域基本上跨越生物传感器的所有活性区域。 在一些实施例中，流体通道基本上与生物传感器的有效区域重叠。 还公开了用于制造和操作微流控盒的方法。

    Disposable, integrated microfluidic cartridge and methods of making and using same
    4.
    发明授权
    Disposable, integrated microfluidic cartridge and methods of making and using same 有权

    公开(公告)号：US11174513B2

    公开(公告)日：2021-11-16

    申请号：US16405547

    申请日：2019-05-07

    Applicant: ILLUMINA, INC.

    Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

    IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/68

    Abstract: A stack of fluidics layers of a microfluidic cartridge for sequencing nucleic acid molecules includes a sequencing chamber layer having a sequencing chamber area configured for carrying out clustering and sequencing reactions, and a sequencing chamber bottom layer disposed under the sequencing chamber layer. The sequencing chamber bottom layer has an opening configured to hold an image sensor with the image sensor having an active area disposed under the sequencing chamber area. The sequencing chamber area spans substantially all of the active area of the image sensor. The stack of fluidics layers includes a flexible printed circuit board (PCB) layer under the sequencing chamber bottom layer, and a fluidics channels layer disposed under the flexible PCB layer. The fluidics channels layer includes fluidics channels that are configured to deliver reactants to the sequencing chamber area. The fluidics channels do not substantially overlap with the active area of the image sensor.

    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME
    5.
    发明申请
    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME 审中-公开

    公开(公告)号：US20190264276A1

    公开(公告)日：2019-08-29

    申请号：US16405547

    申请日：2019-05-07

    Applicant: ILLUMINA, INC.

    Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

    IPC: C12Q1/6869 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6806

    Abstract: A stack of fluidics layers of a microfluidic cartridge for sequencing nucleic acid molecules includes a sequencing chamber layer having a sequencing chamber area configured for carrying out clustering and sequencing reactions, and a sequencing chamber bottom layer disposed under the sequencing chamber layer. The sequencing chamber bottom layer has an opening configured to hold an image sensor with the image sensor having an active area disposed under the sequencing chamber area. The sequencing chamber area spans substantially all of the active area of the image sensor. The stack of fluidics layers includes a flexible printed circuit board (PCB) layer under the sequencing chamber bottom layer, and a fluidics channels layer disposed under the flexible PCB layer. The fluidics channels layer includes fluidics channels that are configured to deliver reactants to the sequencing chamber area. The fluidics channels do not substantially overlap with the active area of the image sensor.

    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
    9.
    发明申请
    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS 有权

    公开(公告)号：US20220243269A1

    公开(公告)日：2022-08-04

    申请号：US17720187

    申请日：2022-04-13

    Applicant: ILLUMINA, INC.

    Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

    IPC: C12Q1/6874 ,  C12Q1/6813

    Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

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