公开(公告)号：US20240117416A1

公开(公告)日：2024-04-11

申请号：US18274974

申请日：2022-01-28

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kuman Khurana ,  Jonathan Mark Boutell

IPC: C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6874

CPC classification number: C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6874

Abstract: The disclosure relates to methods, compositions, and kits for improving seeding efficiency of flow cells with polynucleotides, and applications thereof, including for sequencing.

公开(公告)号：US20220106588A1

公开(公告)日：2022-04-07

申请号：US17451771

申请日：2021-10-21

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6834

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US20220411859A1

公开(公告)日：2022-12-29

申请号：US16609727

申请日：2018-08-01

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Falko Noé ,  Charles Lin ,  Tarun Kumar Khurana ,  Foad Mashayekhi ,  Pallavi Daggumati ,  Frank J Steemers ,  Kevin Gunderson ,  Jeffrey S Fisher

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/6806

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating genetic material. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US20200216895A1

公开(公告)日：2020-07-09

申请号：US16626115

申请日：2018-07-31

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20230242967A1

公开(公告)日：2023-08-03

申请号：US18013788

申请日：2021-07-02

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Jeff Fisher ,  Jeff Brodin

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6806 ,  C12N15/1068 ,  C12Q1/6876 ,  C12Q1/6851 ,  C12Q2600/16

Abstract: The disclosure provides methods to calibrate polynucleotide seeding efficiency in flow cells.

公开(公告)号：US11352668B2

公开(公告)日：2022-06-07

申请号：US16626115

申请日：2018-07-31

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20230220462A1

公开(公告)日：2023-07-13

申请号：US18180622

申请日：2023-03-08

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

CPC classification number: C12Q1/6874 ,  C12Q1/6813 ,  C12Q2600/156 ,  C12Q2565/514

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US11180752B2

公开(公告)日：2021-11-23

申请号：US16272870

申请日：2019-02-11

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6834 ,  C12Q1/6806 ,  B01L7/00

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.

公开(公告)号：US20200325467A1

公开(公告)日：2020-10-15

申请号：US16626221

申请日：2018-06-28

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Brian D. Mather ,  Cyril Delattre ,  Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Pallavi Daggumati ,  Behnam Javanmardi ,  Filiz Gorpe-Yasar ,  Sebastien Georg Gabriel Ricoult ,  Xavier von Hatten ,  Daniel Leonard Fuller

IPC: C12N15/10 ,  C08F226/10

Abstract: An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.

公开(公告)号：US20190249171A1

公开(公告)日：2019-08-15

申请号：US16272870

申请日：2019-02-11

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Filiz Gorpe-Yasar ,  Tarun Kumar Khurana ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12N15/1068 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/123 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2527/156 ,  C12Q2531/119 ,  C12Q2535/122 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.