SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS

    公开(公告)号:US20220243269A1

    公开(公告)日:2022-08-04

    申请号:US17720187

    申请日:2022-04-13

    Applicant: ILLUMINA, INC.

    Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

    OBTAINING INFORMATION FROM A BIOLOGICAL SAMPLE IN A FLOW CELL

    公开(公告)号:US20240060954A1

    公开(公告)日:2024-02-22

    申请号:US18385442

    申请日:2023-10-31

    Applicant: ILLUMINA, INC.

    CPC classification number: G01N33/4836 G01N33/5047 G01N33/5308 A61K39/12

    Abstract: Methods are used for obtaining, cataloguing, and/or storing data derived from a biological source using a flow cell body, electrodes, and an imaging assembly. The data may include DNA and/or RNA obtained from a biological source, such as from the cells of an organism. The methods may be used to obtain, catalog, and/or store data such as DNA or RNA sequence from a pathogen such as a virus and/or a bacteria, human health data over time, and immune system information from an individual. The data obtained using the disclosed methods may be used for a variety of different purposes, including the manufacture of vaccine compositions, and for restoring the immune system of an individual who has undergone an immune system depleting event. The methods may be used for storage of biological cells, which may be used for the screening of compounds, such as small molecules with potential for therapeutic indications.

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