公开(公告)号：US10597653B2

公开(公告)日：2020-03-24

申请号：US15489549

申请日：2017-04-17

Applicant: Illumina, Inc.

Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

IPC: C12N15/10 ,  C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: The invention provides methods for generating a nucleic acid library. Target-specific primer-probes are hybridized to a target nucleic acid fragment to create a hybridization product. Each of the target-specific primer-probes comprises a target-specific sequence and a first adaptor sequence. The target nucleic acid fragment comprises a target genomic region of interest, wherein said target genomic region of interest comprises an exon of a gene and the target-specific primer-probes are tiled across the exon of the gene. The target nucleic acid fragment further comprises a second adaptor sequence different from said first adaptor sequence. Following hybridization, the target-specific primer-probes are extended to create double-stranded extension products and further amplified, thereby generating the nucleic acid library.

公开(公告)号：US11634707B2

公开(公告)日：2023-04-25

申请号：US15549334

申请日：2016-02-10

Applicant: Illumina, Inc.

Inventor： Kevin L Gunderson ,  Frank J Steemers ,  Jeffrey S Fisher ,  Roberto Rigatti

IPC: C12N15/10 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Embodiments of the present invention relate to analyzing components of a cell. In some embodiments, the present invention relate to analyzing components of a single cell. In some embodiments, the methods and compositions relate to sequencing nucleic acids. In some embodiments, the methods and compositions relate to identifying and/or quantitating nucleic acid, proteins, organelles, and/or cellular metabolites.

公开(公告)号：US11142759B2

公开(公告)日：2021-10-12

申请号：US16786198

申请日：2020-02-10

Applicant: Illumina, Inc.

Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6874

Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

公开(公告)号：US09944924B2

公开(公告)日：2018-04-17

申请号：US14796753

申请日：2015-07-10

Applicant: ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

Inventor： Roberto Rigatti ,  Niall Anthony Gormley ,  Allen E. Eckhardt ,  Jonathan Mark Boutell

IPC: C12N15/10 ,  C12Q1/68 ,  C40B50/06

CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6853 ,  C12Q1/6874 ,  C40B50/06 ,  C12Q2525/204 ,  C12Q2565/543

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.

公开(公告)号：US09624489B2

公开(公告)日：2017-04-18

申请号：US14611991

申请日：2015-02-02

Applicant: Illumina, Inc.

Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

IPC: C12Q1/68 ,  C12N15/10 ,  C07H21/02

CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

公开(公告)号：US20150148263A1

公开(公告)日：2015-05-28

申请号：US14611991

申请日：2015-02-02

Applicant: Illumina, Inc.

Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

IPC: C12N15/10

CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

Abstract translation: 本发明提供了控制固体支持物表面上不同分子种类的密度的方法。 将不同分子种类的第一混合物连接到固体支持物上，以便以期望的密度附着每种物质，从而产生具有附着捕获分子的衍生化载体。 衍生的载体用不同分子种类的第二混合物处理，其中第二混合物中的不同分子物质特异性地结合到固相载体上的不同捕获分子。 一个或多个捕获分子可以被可逆地修饰，使得捕获分子在分子种类的第二混合物附着之前和之后具有不同的活性。 在具体实施方案中，不同的分子种类是可逆修饰以在扩增反应中具有不同活性的核酸。