公开(公告)号：US20240026348A1

公开(公告)日：2024-01-25

申请号：US18476719

申请日：2023-09-28

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Susan C. Verity ,  Robert Scott Kuersten ,  Niall Anthony Gormley ,  Andrew B. Kennedy ,  Sarah E. Shultzaberger ,  Andrew Slatter ,  Emma Bell ,  Sebastien Georg Gabriel Ricoult ,  Grace DeSantis ,  Fiona Kaper ,  Han-Yu Chuang ,  Oliver Jon Miller ,  Jason Richard Betley ,  Stephen M. Gross ,  Mats Ekstrand

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/1068

摘要： Materials and methods for preparing nucleic acid libraries for next-generation sequencing are described herein. A variety of approaches are described relating to the use of unique molecular identifiers with transposon-based technology in the preparation of sequencing libraries. Also described herein are sequencing materials and methods for identifying and correcting amplification and sequencing errors.

公开(公告)号：US20230407388A1

公开(公告)日：2023-12-21

申请号：US18303905

申请日：2023-04-20

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Tarun Khurana ,  Yir-Shyuan Wu ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

IPC分类号： C12Q1/6874

CPC分类号： C12Q1/6874

摘要： Described herein is a polynucleotide for use as a sequencing template comprising multiple inserts. Also described herein are method of generating and using these polynucleotides and methods of use of such templates, including analysis of contiguity information. Further, sequencing templates comprising an insert sequence and a copy of the insert sequence can be used to correct for random errors generated during sequencing or amplification or to identify nucleobase damage or other mutation that leads to non-canonical base pairing in a double-stranded nucleic acid. Methods of performing methylation analysis are also described herein.

公开(公告)号：US20230183682A1

公开(公告)日：2023-06-15

申请号：US18163405

申请日：2023-02-02

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Niall Anthony Gormley ,  Andrew B. Kennedy ,  Robert Scott Kuersten ,  Gary Schroth ,  Carlo Randise-Hinchliff ,  Sarah E. Shultzaberger ,  Fiona Kaper ,  Yir-Shyuan Wu ,  Tarun Khurana ,  Foad Mashayekhi

IPC分类号： C12N15/10

CPC分类号： C12N15/1065

摘要： This application describes methods of preparing an immobilized library of tagged RNA fragments. Also described herein are a number of methods of preparing DNA and RNA sequencing libraries from a single sample. These methods can include library preparation from single cells.

公开(公告)号：US09944924B2

公开(公告)日：2018-04-17

申请号：US14796753

申请日：2015-07-10

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC.

发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Allen E. Eckhardt ,  Jonathan Mark Boutell

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C40B50/06

CPC分类号： C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6853 ,  C12Q1/6874 ,  C40B50/06 ,  C12Q2525/204 ,  C12Q2565/543

摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.

公开(公告)号：US20230279469A1

公开(公告)日：2023-09-07

申请号：US18146320

申请日：2022-12-23

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC. ,  Illumina Singapore Pte. Ltd.

发明人： Johan Sebastian Basuki ,  Jonathan Mark Boutell ,  Jeffrey S. Fisher ,  Louise Jane Fraser ,  Wayne N. George ,  Niall Anthony Gormley ,  David Jones ,  Xiaoyu Ma ,  Maria Ines Martins Vitoriano ,  Zhong Mei ,  Oliver Jon Miller ,  Andrew Price ,  Sebastien Georg Gabriel Ricoult ,  Vicki S. Thomson ,  Jacqueline C. Weir ,  Xiaoy Ma ,  Weihua Chang ,  Hui Han

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  B01L3/00

CPC分类号： C12Q1/6806 ,  B01L3/502761 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2300/0893

摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.

公开(公告)号：US09677132B2

公开(公告)日：2017-06-13

申请号：US14575863

申请日：2014-12-18

申请人： Illumina Cambridge Limited ,  Illumina, Inc.

发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Allen E. Eckhardt ,  Jonathan Mark Boutell

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2535/122 ,  C12Q2565/543

摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.

公开(公告)号：US11685946B2

公开(公告)日：2023-06-27

申请号：US16739739

申请日：2020-01-10

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Andrew Slatter ,  Esther Musgrave-Brown ,  Susan C. Verity ,  Niall Anthony Gormley

IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

CPC分类号： C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869

摘要： The present disclosure relates to methods, compositions, and kits for generating a library of tagged nucleic acid fragments without using PCR amplification, including methods and compositions for fragmenting and tagging nucleic acids (e.g., DNA) using transposome complexes immobilized on solid support.

公开(公告)号：US20220403376A1

公开(公告)日：2022-12-22

申请号：US17890632

申请日：2022-08-18

申请人： Illumina Cambridge Limited

发明人： Niall Anthony Gormley ,  Avgousta Ioannou ,  Rosamond Jackson ,  Natalie Morrell

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12Q1/6806 ,  C40B50/14

摘要： Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.

公开(公告)号：US20200002747A1

公开(公告)日：2020-01-02

申请号：US16561483

申请日：2019-09-05

申请人： Illumina Cambridge Limited

发明人： Roberto Rigatti ,  Niall Anthony Gormley

IPC分类号： C12Q1/6806 ,  C12Q1/6874

摘要： Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid in contact with a surface, and sequencing the library on the surface

公开(公告)号：US10329613B2

公开(公告)日：2019-06-25

申请号：US14683580

申请日：2015-04-10

申请人： Illumina Cambridge Limited

发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

IPC分类号： C12P19/34 ,  C12Q1/6874

摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.