公开(公告)号：US10767219B2

公开(公告)日：2020-09-08

申请号：US15125124

申请日：2015-03-11

Applicant: Illumina, Inc.

Inventor： Poorya Sabounchi ,  Behnam Javanmardi ,  Tarun Khurana ,  Philip Paik ,  Yan-You Lin

IPC: B01L3/00 ,  C12Q1/6869 ,  B01L7/00 ,  C12Q1/6806 ,  C40B60/06

Abstract: The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

公开(公告)号：US20220275443A1

公开(公告)日：2022-09-01

申请号：US17662124

申请日：2022-05-05

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Yan-You Lin ,  M. Shane Bowen ,  Cyril Delattre ,  Fabien Abeille ,  Tarun Khurana ,  Arnaud Rival ,  Poorya Sabounchi ,  Dajun Yuan ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/6874 ,  B05D3/06 ,  C12Q1/6806 ,  G03F7/00 ,  G03F7/20

Abstract: Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

公开(公告)号：US20210139979A1

公开(公告)日：2021-05-13

申请号：US17153379

申请日：2021-01-20

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Yan-You Lin ,  M. Shane Bowen ,  Cyril Delattre ,  Fabien Abeille ,  Tarun Khurana ,  Arnaud Rival ,  Poorya Sabounchi ,  Dajun Yuan ,  Maria Candelaria Rogert Bacigalupo

IPC: C12Q1/6874 ,  B05D3/06 ,  C12Q1/6806 ,  G03F7/00 ,  G03F7/20

Abstract: Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

公开(公告)号：US10900076B2

公开(公告)日：2021-01-26

申请号：US16096669

申请日：2017-05-17

Applicant: Illumina, Inc.

Inventor： Yir-Shyuan Wu ,  Yan-You Lin ,  M. Shane Bowen ,  Cyril Delattre ,  Fabien Abeille ,  Tarun Khurana ,  Arnaud Rival ,  Poorya Sabounchi ,  Dajun Yuan ,  Maria Candelaria Rogert Bacigalupo

IPC: G03F7/20 ,  C12Q1/6874 ,  B05D3/06 ,  C12Q1/6806 ,  G03F7/00

Abstract: Embodiments provided herewith are directed to self-assembled methods of preparing a patterned surface for sequencing applications including, for example, a patterned flow cell or a patterned surface for digital fluidic devices. The methods utilize photolithography to create a patterned surface with a plurality of microscale or nanoscale contours, separated by hydrophobic interstitial regions, without the need of oxygen plasma treatment during the photolithography process. In addition, the methods avoid the use of any chemical or mechanical polishing steps after the deposition of a gel material to the contours.

公开(公告)号：US20200216895A1

公开(公告)日：2020-07-09

申请号：US16626115

申请日：2018-07-31

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.