公开(公告)号：US09995683B2

公开(公告)日：2018-06-12

申请号：US14308477

申请日：2014-06-18

Applicant: Industrial Technology Research Institute

Inventor： Chung-Fan Chiou ,  Rung-Ywan Tsai ,  Yu-Tang Li ,  Chih-Tsung Shih ,  Ming-Chia Li ,  Chang-Sheng Chu ,  Shuang-Chao Chung ,  Jung-Po Chen ,  Ying-Chih Pu

IPC: G01N21/00 ,  G01N21/64 ,  B82Y15/00 ,  G01N21/77 ,  G01N33/543

CPC classification number: G01N21/6486 ,  B82Y15/00 ,  G01N21/645 ,  G01N21/648 ,  G01N21/7703 ,  G01N33/54373 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/088 ,  Y10S977/954 ,  Y10S977/957 ,  Y10S977/958 ,  Y10T436/143333 ,  Y10T436/145555

Abstract: An apparatus for detecting an object capable of emitting light. The apparatus includes a light source and a waveguide. The waveguide includes a core layer and a first cladding layer. At least one nanowell is formed in at least the first cladding layer. The apparatus further includes a light detector. The light detector can detect a light emitted from a single molecule object contained in the at least one nanowell.

公开(公告)号：US09777321B2

公开(公告)日：2017-10-03

申请号：US15260773

申请日：2016-09-09

Applicant: Industrial Technology Research Institute

Inventor： Chung-Fan Chiou ,  Ying-Chih Pu ,  Chao-Chi Pan ,  Chih-Tsung Shih ,  Ming-Chia Li ,  Chein-Shiu Kuo ,  Hung-Chi Chien ,  Chang-Sheng Chu

IPC: G01N21/00 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/77

CPC classification number: C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6454 ,  G01N21/648 ,  G01N21/7703 ,  G01N2021/6439 ,  G01N2201/08 ,  G01N2201/12 ,  C12Q2563/155 ,  C12Q2561/113

Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.

公开(公告)号：US09747414B2

公开(公告)日：2017-08-29

申请号：US14286904

申请日：2014-05-23

Applicant: Industrial Technology Research Institute

Inventor： Chao-Chi Pan ,  Jenn-Yeh Fann ,  Chung-Fan Chiou ,  Hung-Chi Chien ,  Hui-Ling Chen

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  C12Q2531/125 ,  C12Q2537/165

Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.

公开(公告)号：US20150379194A1

公开(公告)日：2015-12-31

申请号：US14717996

申请日：2015-05-20

Applicant: Industrial Technology Research Institute

Inventor： Chao-Chi Pan ,  Jenn-Yeh Fann ,  Chung-Fan Chiou ,  Hung-Chi Chien ,  Hui-Ling Chen

IPC: G06F19/22

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  C12Q2531/125 ,  C12Q2537/165

Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.

Abstract translation: 本文公开了在具有已知序列的核酸插入物的环状分子中存在的核酸样品中修饰碱基的序列和/或位置的方法，包括获得至少两个插入样品单元的序列数据。 在一些实施方案中，所述方法包括使用环状双锁定分子获得序列数据。 在一些实施方案中，所述方法包括通过将序列与核酸插入物的已知序列进行比较来计算核酸插入序列的分数，以及根据一个或多个核酸插入片段的得分接受或排除核酸样品序列的重复序列 插入物的两个序列紧接着核酸样品序列的重复序列的上游或下游。