公开(公告)号：US11053507B2

公开(公告)日：2021-07-06

申请号：US17072023

申请日：2020-10-15

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20210147858A1

公开(公告)日：2021-05-20

申请号：US16952024

申请日：2020-11-18

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N9/22 ,  C12N1/20

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

公开(公告)号：US20200056192A1

公开(公告)日：2020-02-20

申请号：US16540767

申请日：2019-08-14

Applicant: Inscripta, Inc.

Inventor： Eileen Spindler ,  Amy Hiddessen ,  Phillip Belgrader ,  Charles Johnson ,  Clint Davis

IPC: C12N15/70 ,  C12M3/00 ,  C12M1/42 ,  C12M1/12 ,  C12N15/90

Abstract: The present disclosure provides methods, automated modules, and instruments for enrichment of live cells that have been edited by nucleic acid-guided nuclease genome editing. The disclosure provides improved methods and modules—including high throughput methods and modules—for enriching for cells that have been subjected to editing.

公开(公告)号：US20190292568A1

公开(公告)日：2019-09-26

申请号：US16358461

申请日：2019-03-19

Applicant: Inscripta, Inc.

Inventor： Christopher Abraham ,  Charles Johnson ,  Clint Davis ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.

公开(公告)号：US20220195443A1

公开(公告)日：2022-06-23

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20210040489A1

公开(公告)日：2021-02-11

申请号：US17072023

申请日：2020-10-15

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US10752874B2

公开(公告)日：2020-08-25

申请号：US16686066

申请日：2019-11-15

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Miles Gander ,  Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Matthew Estes ,  Bruce Chabansky ,  Jorge Bernate

IPC: C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  C12N15/87 ,  B01D25/00 ,  B01D63/08 ,  B01D29/05 ,  B01D71/02 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200071657A1

公开(公告)日：2020-03-05

申请号：US16686066

申请日：2019-11-15

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Miles Gander ,  Don Masquelier ,  Phillip Belgrader ,  Andrew Garst ,  Matthew Estes ,  Bruce Chabansky

IPC: C12M1/00 ,  C12N15/87 ,  C12M3/00 ,  C12M1/26

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.