公开(公告)号：US11634719B2

公开(公告)日：2023-04-25

申请号：US17676210

申请日：2022-02-20

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian Tian ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20230049357A1

公开(公告)日：2023-02-16

申请号：US17969715

申请日：2022-10-20

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US11319542B2

公开(公告)日：2022-05-03

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

公开(公告)号：US11306299B2

公开(公告)日：2022-04-19

申请号：US17518556

申请日：2021-11-03

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/85 ,  C12Q1/44

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20220081693A1

公开(公告)日：2022-03-17

申请号：US17536067

申请日：2021-11-28

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Eileen Spindler ,  Charles Johnson ,  Clint Davis

IPC: C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  C12N9/22

Abstract: The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of “edit or die.” Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome

公开(公告)号：US20210310012A1

公开(公告)日：2021-10-07

申请号：US17353282

申请日：2021-06-21

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US11136572B2

公开(公告)日：2021-10-05

申请号：US17230241

申请日：2021-04-14

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20230012154A1

公开(公告)日：2023-01-12

申请号：US17679203

申请日：2022-02-24

Applicant: Inscripta, Inc.

Inventor： Miles Gander ,  Tian Tian ,  Skylar Stefani ,  Patrick Westfall

IPC: C12N15/10

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US20220195409A1

公开(公告)日：2022-06-23

申请号：US17692108

申请日：2022-03-10

Applicant: Inscripta, Inc.

Inventor： Skylar Stefani ,  Tian Tian ,  Miles Gander

IPC: C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

公开(公告)号：US11254942B2

公开(公告)日：2022-02-22

申请号：US17353282

申请日：2021-06-21

Applicant: Inscripta, Inc.

Inventor： Tian Tian ,  Charles Johnson ,  Eileen Spindler

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.