公开(公告)号：US10059975B2

公开(公告)日：2018-08-28

申请号：US12589929

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/359

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

公开(公告)号：US20100129857A1

公开(公告)日：2010-05-27

申请号：US12589929

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  C12N1/00

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供了在单个系统内原位分离，表征和/或鉴定微生物的方法。

公开(公告)号：US20100129814A1

公开(公告)日：2010-05-27

申请号：US12589985

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/68 ,  G01N33/567 ,  C12Q1/37 ,  C12Q1/04

CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

公开(公告)号：US09128058B2

公开(公告)日：2015-09-08

申请号：US12589985

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64

CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

公开(公告)号：US20100124763A1

公开(公告)日：2010-05-20

申请号：US12589968

申请日：2009-10-30

申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3577 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

摘要翻译： 本发明提供了用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统。 该方法可以在密封容器中通过利用第一步骤时间依赖性光谱技术来实现，以获得包含样品的生长组合物的至少两个测量值，并将所述测量结果与生物学颗粒的检测和/或表征相关联，生物颗粒可以是 存在于样品中。 该方法还提供了在密封容器中分离，表征和/或鉴定微生物的后续步骤。

公开(公告)号：US08748122B2

公开(公告)日：2014-06-10

申请号：US12653527

申请日：2009-12-15

申请人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04

摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

公开(公告)号：US20130323718A1

公开(公告)日：2013-12-05

申请号：US13959802

申请日：2013-08-06

申请人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04

摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

摘要翻译： 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US08795983B2

公开(公告)日：2014-08-05

申请号：US13959802

申请日：2013-08-06

申请人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04

摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

摘要翻译： 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US20120156716A1

公开(公告)日：2012-06-21

申请号：US13328379

申请日：2011-12-16

申请人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

IPC分类号： C12Q1/04 ,  C12M1/00 ,  B01D35/00

CPC分类号： B01L3/50255 ,  B01L3/561 ,  B01L2300/0681

摘要： The present invention is directed to methods, devises and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. In one embodiment, a filtration and sample transfer device is used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation microorganisms, the isolated and/or accumulated microorganisms can be analyzing to acquire measurements for the characterization and/or identification of said microorganisms.

摘要翻译： 本发明涉及用于分离，积累，表征和/或鉴定已知存在或可能存在于测试样品中的微生物的方法，设计和试剂盒。 本发明的方法包括任选地裂解可能存在于测试样品中的非微生物细胞和/或颗粒，随后进行用于分离和/或积累微生物的随后的过滤步骤。 在一个实施方案中，过滤和样品转移装置用于通过过滤分离和/或积累微生物。 一旦微生物已被过滤以分离和/或累积微生物，分离的和/或累积的微生物可以被分析以获得用于表征和/或鉴定所述微生物的测量。

公开(公告)号：US10335785B2

公开(公告)日：2019-07-02

申请号：US13328379

申请日：2011-12-16

申请人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

IPC分类号： B01L3/00

摘要： The present invention is directed to methods, devices and kits for separating, accumulating, characterizing and/or identifying microorganisms in a test sample. The method of the invention includes optionally lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The filtration and sample transfer device may be used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation, the microorganisms can be analyzed to acquire measurements for the characterization and/or identification of said microorganisms.