公开(公告)号：US10415075B2

公开(公告)日：2019-09-17

申请号：US12589936

申请日：2009-10-30

申请人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  Bradford Clay ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  G01N33/68 ,  H01J49/00

摘要： A method is described to separate, characterize and/or identify microorganisms in a test sample. Said method comprises the steps of (i) lysing non-microorganism cells that might be present in the sample, (ii) separating the microorganisms as a pellet in the sample post-lysing non-microorganism cells, (iii) interrogating the pellet containing microorganisms by mass spectrometry to produce mass spectra of microorganisms, and (iv) characterizing and/or identifying microorganisms in the sample. The method may be used in characterizing/identifying microorganisms in complex samples, e.g., blood containing culture media.

公开(公告)号：US09175329B2

公开(公告)日：2015-11-03

申请号：US14090169

申请日：2013-11-26

申请人： John Walsh ,  Jones Hyman

发明人： John Walsh ,  Jones Hyman

IPC分类号： C12Q1/14 ,  C12Q1/04 ,  C12Q1/02 ,  G01N33/569

CPC分类号： C12Q1/04 ,  C12Q1/025 ,  G01N33/56911

摘要： The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system.

摘要翻译： 本发明涉及用于测定微生物抗生素抗性状态的方法和系统。 本发明还提供了在单个系统内测定原位微生物的抗生素抗性状态的方法。

公开(公告)号：US20140186881A1

公开(公告)日：2014-07-03

申请号：US14200662

申请日：2014-03-07

申请人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

发明人： John Walsh ,  Jones Hyman ,  Bradford Clay ,  Thurman Thorpe

IPC分类号： C12Q1/04 ,  C12Q1/10

CPC分类号： C12Q1/04 ,  C12Q1/10 ,  G01N21/31 ,  G01N21/47 ,  G01N21/64 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421

摘要： The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.

摘要翻译： 本发明提供了一种用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统，其中所述方法可以非侵入地通过利用时间依赖的光谱技术来获得至少两个测量 的生长组合物，其包含样品并将所述测量结果与可能存在于样品中的生物颗粒的检测和/或表征相关联。

公开(公告)号：US20140087368A1

公开(公告)日：2014-03-27

申请号：US14090169

申请日：2013-11-26

申请人： John Walsh ,  Jones Hyman

发明人： John Walsh ,  Jones Hyman

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  C12Q1/025 ,  G01N33/56911

摘要： The present invention relates to methods and systems for determining the antibiotic-resistance status of microorganisms. The invention further provides methods for determining the antibiotic-resistance status of microorganisms in situ within a single system.

摘要翻译： 本发明涉及用于测定微生物抗生素抗性状态的方法和系统。 本发明还提供了在单个系统内测定原位微生物的抗生素抗性状态的方法。

公开(公告)号：US20120156716A1

公开(公告)日：2012-06-21

申请号：US13328379

申请日：2011-12-16

申请人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

IPC分类号： C12Q1/04 ,  C12M1/00 ,  B01D35/00

CPC分类号： B01L3/50255 ,  B01L3/561 ,  B01L2300/0681

摘要： The present invention is directed to methods, devises and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. In one embodiment, a filtration and sample transfer device is used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation microorganisms, the isolated and/or accumulated microorganisms can be analyzing to acquire measurements for the characterization and/or identification of said microorganisms.

摘要翻译： 本发明涉及用于分离，积累，表征和/或鉴定已知存在或可能存在于测试样品中的微生物的方法，设计和试剂盒。 本发明的方法包括任选地裂解可能存在于测试样品中的非微生物细胞和/或颗粒，随后进行用于分离和/或积累微生物的随后的过滤步骤。 在一个实施方案中，过滤和样品转移装置用于通过过滤分离和/或积累微生物。 一旦微生物已被过滤以分离和/或累积微生物，分离的和/或累积的微生物可以被分析以获得用于表征和/或鉴定所述微生物的测量。

公开(公告)号：US09790534B2

公开(公告)日：2017-10-17

申请号：US14146367

申请日：2014-01-02

申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04 ,  G01N33/68 ,  H01J49/00

CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

公开(公告)号：US08795983B2

公开(公告)日：2014-08-05

申请号：US13959802

申请日：2013-08-06

申请人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

发明人： Jones Hyman ,  John Walsh ,  Thurman Thorpe

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04

摘要： The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

摘要翻译： 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US10335785B2

公开(公告)日：2019-07-02

申请号：US13328379

申请日：2011-12-16

申请人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

IPC分类号： B01L3/00

摘要： The present invention is directed to methods, devices and kits for separating, accumulating, characterizing and/or identifying microorganisms in a test sample. The method of the invention includes optionally lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The filtration and sample transfer device may be used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation, the microorganisms can be analyzed to acquire measurements for the characterization and/or identification of said microorganisms.

公开(公告)号：US20140186879A1

公开(公告)日：2014-07-03

申请号：US14146367

申请日：2014-01-02

申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US08647835B2

公开(公告)日：2014-02-11

申请号：US13117976

申请日：2011-05-27

申请人： John Walsh ,  Jones Hyman

发明人： John Walsh ,  Jones Hyman

IPC分类号： C12Q1/04

CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/6486 ,  G01N21/65 ,  G01N2015/1402

摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements.

摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 该方法还可用于混合测试样品中包含的两种或多种不同或单独的微生物物质的物理分离和/或富集。 本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。