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公开(公告)号：US07098032B2
公开(公告)日：2006-08-29
申请号：US11046590
申请日：2005-01-28
申请人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
发明人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/60 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07098030B2
公开(公告)日：2006-08-29
申请号：US10899509
申请日：2004-07-26
申请人： David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
发明人： David B. Rozema , Vladimir G. Budker , James E. Hagstrom , Vladimir Trubetskoy , Jon A. Wolff , Sean D. Monahan , Paul M. Slattum
IPC分类号： C12N15/88
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/645
摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07033607B2
公开(公告)日：2006-04-25
申请号：US10095789
申请日：2002-03-11
申请人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
发明人： Vladimir S. Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jon A. Wolff , David B. Rozema , Sean D. Monahan
IPC分类号： A61K9/127 , A61K31/713
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
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公开(公告)号：US07589059B2
公开(公告)日：2009-09-15
申请号：US10628792
申请日：2003-07-28
申请人： Jon A. Wolff , Vladimir G. Budker , Hans Herweijer , James E. Hagstrom , Sean D. Monahan , Julia Hegge , Vladimir Subbotin
发明人： Jon A. Wolff , Vladimir G. Budker , Hans Herweijer , James E. Hagstrom , Sean D. Monahan , Julia Hegge , Vladimir Subbotin
CPC分类号： A61K9/0019 , A61K38/00 , A61K48/0075 , C12N15/87 , C12N2799/022
摘要： Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.
摘要翻译： 公开了一种使用血管内施用途径向血管外哺乳动物细胞体内递送分子或复合物的系统。 将分子或复合物插入哺乳动物脉管系统的注射溶液中。 以适当的速率插入注射溶液瞬时增加组织中血管外流体的体积，从而便于将分子递送至细胞。
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公开(公告)号：US07091041B2
公开(公告)日：2006-08-15
申请号：US10627247
申请日：2003-07-25
CPC分类号： A61K48/0025 , A61K9/1075
摘要： A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
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公开(公告)号：US06673612B2
公开(公告)日：2004-01-06
申请号：US10081461
申请日：2002-02-21
IPC分类号： C12N1588
CPC分类号： A61K48/0025 , A61K9/1075
摘要： A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
摘要翻译： 描述了可递送至细胞的复合物，包括将核酸或其他货物插入反胶束中。 反胶束具有压实核酸以便于递送的性质。
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公开(公告)号：US06429200B1
公开(公告)日：2002-08-06
申请号：US09354957
申请日：1999-07-16
IPC分类号： A61K317105
CPC分类号： A61K9/1075 , A61K48/0025
摘要： A complex is described for delivery to a cell comprising inserting a nucleic acid into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery. Other molecules are used to interact with the nucleic acid—micelle complex to further enhance delivery such as a surfactant having a disulfide bond.
摘要翻译： 描述了一种复合物用于递送至包含将核酸插入反胶束中的细胞。 反胶束具有压实核酸以便于递送的性质。 使用其他分子与核酸 - 胶束复合物相互作用以进一步增强递送，例如具有二硫键的表面活性剂。
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公开(公告)号：US20080234222A1
公开(公告)日：2008-09-25
申请号：US12132030
申请日：2008-06-03
申请人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
IPC分类号： A61K31/70
摘要： An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
摘要翻译： 将离子聚合物用于“再充电”（具有不同电荷的另一层）缩合的多核苷酸复合物，用于向细胞递送核酸。 可以用合适量的正电荷或负电荷形成所得的再充电络合物，使得所得复合物具有所需的净电荷。
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公开(公告)号：US07396919B1
公开(公告)日：2008-07-08
申请号：US09328975
申请日：1999-06-09
申请人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir S. Trubetskoy , Aaron G. Loomis , Paul M. Slattum , Sean D. Monahan , James E. Hagstrom , Vladimir G. Budker
摘要： An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
摘要翻译： 将离子聚合物用于“再充电”（具有不同电荷的另一层）缩合的多核苷酸复合物，用于向细胞递送核酸。 可以用合适量的正电荷或负电荷形成所得的再充电络合物，使得所得复合物具有所需的净电荷。
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公开(公告)号：US07470539B2
公开(公告)日：2008-12-30
申请号：US11256322
申请日：2005-10-21
申请人： Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
发明人： Darren H. Wakefield , David B. Rozema , Jon A. Wolff , Vladimir Trubetskoy , James E. Hagstrom , Vladimir G. Budker , Jason Klein , So Wong
IPC分类号： C12N15/88
CPC分类号： C12N15/87 , A61K47/6455 , C12N15/88
摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
摘要翻译： 聚阴离子能够冷凝核酸以形成可用于将核酸递送至哺乳动物细胞的小复合物。 聚阴离子可以在与核酸相互作用之前形成，或者可以在核酸存在下形成。 可以修饰稳定的聚阳离子/核酸复合物以减少聚阳离子的正电荷并添加靶向配体而不使复合物不稳定。 所得颗粒保留其小尺寸并且在体内将核酸递送至细胞更有效。
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