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公开(公告)号：US20210332332A1
公开(公告)日：2021-10-28
申请号：US16490515
申请日：2019-05-02
Inventor： Sang Yup LEE , Kyung-Jin KIM , Jung Ho AHN , Hogyun SEO , Jong An LEE
Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.
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公开(公告)号：US20220136028A1
公开(公告)日：2022-05-05
申请号：US17515614
申请日：2021-11-01
Inventor： Sang Yup LEE , Dongsoo Yang , Seon Young Park
Abstract: Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.
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13.发明申请公开(公告)号：US20210375400A1
公开(公告)日：2021-12-02
申请号：US16766690
申请日：2018-11-20
Inventor： Sang Yup LEE , Jae Yong RYU , Hyun Uk KIM
Abstract: The present invention relates to a method for predicting a drug-drug interaction and a drug-food interaction by using structural information of a drug and, more particularly, to a method for predicting the mechanism of action and activity of a drug interaction through interaction prediction results expressed by a standardized sentence. When using a method for predicting a drug interaction according to the present invention, a drug interaction can be predicted quickly and accurately, and in particular, activity information of an unknown compound can also be predicted by expressing a prediction result by means of a sentence, and thus the method is very useful for developing a drug exhibiting desired activity without causing adverse effects.
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公开(公告)号：US20210054375A1
公开(公告)日：2021-02-25
申请号：US16960064
申请日：2019-03-08
Inventor： Sang Yup LEE , Dongsoo YANG , Jae Sung CHO
IPC: C12N15/113 , C12N15/77 , C12Q1/689
Abstract: The present disclosure relate to a composition for inhibiting a prokaryotic expression and a use thereof and, more specifically, to a composition for inhibiting an expression of Gram-positive bacteria, which includes an sRNA comprising an sRNA-derived Hfq binding site from prokaryotes and (ii) a region that forms a complementary bond with a target gene mRNA and an Hfq from prokaryotes, a method of producing same, and a use thereof. A synthetic sRNA according to the present disclosure and a composition comprising the sRNA for inhibiting a gene expression having an advantage of being able to control single and multiple target genes at a time, can effectively reduce the expression of the target gene without the conventional gene deletion process via the synthetic sRNA that controls a gene expression so as to be useful for the production of a recombinant microorganism, and are particularly useful for inhibiting a gene expression of Gram-positive bacteria. A recombinant Corynebacterium produced by the present disclosure is a recombinant microorganism capable of mass production of high value products in an ecofriendly and reproducible manner on a bio-basis by controlling microbial metabolism flow through the synthetic sRNA. The recombinant microorganism, which is a bio-based production system developed through the sRNA is useful because of being able to replace existing fossil fuels while resolving environmental problems due to the ever-increasing use of crude oil.
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15.发明申请公开(公告)号：US20200270587A1
公开(公告)日：2020-08-27
申请号：US16792229
申请日：2020-02-15
Inventor： Sang Yup LEE , Zi Wei LUO , Jae Sung CHO
Abstract: Disclosed are a recombinant microorganism capable of producing methyl anthranilate, wherein the recombinant microorganism is obtained by introducing an aamt1 gene into a microorganism having the capacity to produce anthranilic acid (ANT), and a method of producing methyl anthranilate using the same. The recombinant microorganism is capable of producing methyl anthranilate using only a purely biological process from renewable carbon-circulating biomass without chemical catalytic reaction, thus having an advantage of enabling mass production of high value-added methyl anthranilate in a very economical manner based on an environmentally friendly and simple production process.
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Patent Agency Ranking
公开(公告)号：US20190382817A1
公开(公告)日：2019-12-19
申请号：US16483430
申请日：2018-12-12
Inventor： Sang Yup LEE , Xin Rui ZHAO , Kyeong Rok CHOI
Abstract: The present invention relates to a microorganism variant having the ability to extracellularly produce heme, and more particularly to a metabolically engineered microorganism variant having the ability to extracellularly produce heme and a method of producing heme using the same. According to the present invention, heme, an organometallic compound which is increasingly used as a health food or food supplement for the treatment of porphyria, can be extracellularly secreted and produced in high yield using the microorganism variant, but not conventional chemical synthesis or enzymatic synthesis.
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公开(公告)号：US20240102068A1
公开(公告)日：2024-03-28
申请号：US18263127
申请日：2022-01-27
Inventor： Sang Yup LEE , Dongsoo YANG , Woo Dae JANG
CPC classification number: C12P19/44 , C12N9/0004 , C12N9/1025 , C12N9/1288 , C12N9/16 , C12N9/93 , C12Y207/08007 , C12Y301/04052 , C12Y604/01002
Abstract: The present invention relates to novel C-glycosyltransferase variants and a use thereof. The C-glycosyltransferase variants according to the present invention have improved glycosidic bond-forming ability as compared with wild-type C-glycosyltransferase, and thus can increase the glycoside production effects of polyketide groups and pseudo-natural products, particularly type I, II, III polyketide, nonribosomal peptides, phenylpropanoids, and other aromatic natural products, and thus can be useful for the preparation of a drug, a food additive, a nutritional supplement, and the like containing a C-glycoside compound as a constituent ingredient.
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公开(公告)号：US20220008463A1
公开(公告)日：2022-01-13
申请号：US17296301
申请日：2019-11-21
Inventor： Sang Yup LEE , Won Min YOO , Sooncheol KIM , Hannah CHUNG , Jiyong KIM
Abstract: The present invention relates to a pharmaceutical composition for treating wounds, containing a silk protein and a plasma component as active ingredients. According to the present invention, wounds can be more effectively healed by using synergistic effects of a silk protein and a plasma component.
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公开(公告)号：US20210317486A1
公开(公告)日：2021-10-14
申请号：US17194222
申请日：2021-03-06
Inventor： Sang Yup LEE , Dong In KIM , Tong Un CHAE
Abstract: Disclosed is a method of preparing primary amines. More particularly, disclosed are a mutant microorganism transformed with a gene encoding a valine decarboxylase and a method of preparing primary amines from amino acids using the mutant microorganism. The method has an effect of synthesizing, in an environmentally friendly manner, primary amines as precursors for pharmaceuticals and agricultural chemicals using microorganisms, as an alternative to conventional chemical synthesis methods.
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公开(公告)号：US20210062232A1
公开(公告)日：2021-03-04
申请号：US16962213
申请日：2019-01-15
Inventor： Sang Yup LEE , Jae Sung CHO , Je Woong KIM , Yoo Sung KO , Cindy Pricilia Surya PRABOWO , Taehee HAN , Euiduk KIM , Jae Won CHOI , Changhee CHO , Jun Kyu LEE
Abstract: The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.
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