公开(公告)号：US20050130183A1

公开(公告)日：2005-06-16

申请号：US10890350

申请日：2004-07-13

申请人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park

发明人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park

IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  C12M1/00 ,  G01N21/03 ,  G01N21/64 ,  C12M1/34

CPC分类号： G01N21/645 ,  B01L3/50851 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/18 ,  G01N21/0332

摘要： Provided are a real-time PCR monitoring apparatus and method. The real-time PCR monitoring apparatus includes a microchip-type PCR tube that has a PCR solution-containing PCR chamber, a micro-heater that applies heat to the PCR chamber of the PCR tube, a detection unit that detects a PCR product signal based on the amount of a PCR product of the PCR solution, a plurality of modules, each of which includes a cooling fan for lowering the inside air temperature and a control unit for adjusting the temperature of the PCR chamber of the PCR tube by controlling the micro-heater and the cooling fan, and receives the PCR tube, the micro-heater, and the detection unit, a base instrument that includes a power supply unit electrically connected to the modules for power supply and a data communication unit electrically connected to the modules for data communication with the control unit of each of the modules, and a display unit that displays data received from the data communication unit, wherein the control unit of each of the modules independently controls at least one of both the detection unit and the temperature of the PCR chamber of the PCR tube received in each of the modules. Therefore, co-amplification of different samples at different temperature conditions can be carried out and monitored in real time.

摘要翻译： 提供了一种实时PCR监测装置和方法。 实时PCR监视装置包括具有含PCR溶液的PCR室的微芯片型PCR管，向PCR管的PCR室照射热的微加热器，检测基于PCR产物信号的检测单元 关于PCR溶液的PCR产物的量，多个模块，每个模块包括用于降低内部空气温度的冷却风扇和用于通过控制微管来调节PCR管的PCR室的温度的控制单元 加热器和冷却风扇，并且接收PCR管，微加热器和检测单元，基本仪器包括电连接到用于电源的模块的电源单元和与模块电连接的数据通信单元 用于与每个模块的控制单元的数据通信，以及显示单元，显示从数据通信单元接收的数据，其中每个模块的控制单元独立地控制 在每个模块中接收PCR管的PCR室的检测单元和温度中的至少一个。 因此，不同温度条件下不同样品的共扩增可以进行实时监测。

公开(公告)号：US08697433B2

公开(公告)日：2014-04-15

申请号：US12843552

申请日：2010-07-26

申请人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

发明人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

IPC分类号： C12M1/00 ,  C12M1/36 ,  C12M1/38 ,  C12M1/34 ,  C12M3/00

CPC分类号： B01L7/52 ,  B01L3/502715 ,  B01L3/50851 ,  B01L2300/0627 ,  B01L2300/18

摘要： Provided are a PCR module and a multiple PCR system using the same. More particularly, provided are a PCR module with a combined PCR thermal cycler and PCR product detector, and a multiple PCR system using the same.

摘要翻译： 提供了PCR模块和使用该PCR模块的多重PCR系统。 更具体地，提供了具有组合的PCR热循环仪和PCR产物检测器的PCR模块，以及使用其的多重PCR系统。

公开(公告)号：US20050164281A1

公开(公告)日：2005-07-28

申请号：US11080705

申请日：2005-03-15

申请人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

发明人： Kwang-wook Oh ,  Jin-tae Kim ,  Kak Namkoong ,  Chin-sung Park ,  Yoon-kyoung Cho

IPC分类号： B01L3/00 ,  B01L7/00 ,  G01N21/03 ,  G01N21/64 ,  C12Q1/68 ,  C12M1/34 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： G01N21/6486 ,  B01L3/50851 ,  B01L7/52 ,  B01L2300/0627 ,  B01L2300/18 ,  G01N21/0332

摘要： Provided are a DNA PCR module and a multiple PCR system using the same. More particularly, provided are a DNA PCR module with a combined PCR thermal cycler and PCR product detector, and a multiple PCR system using the same.

摘要翻译： 提供了DNA PCR模块和使用其的多重PCR系统。 更具体地，提供了具有组合的PCR热循环仪和PCR产物检测器的DNA PCR模块，以及使用其的多重PCR系统。

公开(公告)号：US20060047443A1

公开(公告)日：2006-03-02

申请号：US11217694

申请日：2005-09-01

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： G06F19/00 ,  C12Q1/68

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

公开(公告)号：US20100221728A1

公开(公告)日：2010-09-02

申请号：US12709774

申请日：2010-02-22

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US08386191B2

公开(公告)日：2013-02-26

申请号：US13161788

申请日：2011-06-16

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： G01N33/48

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US07698072B2

公开(公告)日：2010-04-13

申请号：US11217694

申请日：2005-09-01

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： G06F19/00

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US08340919B2

公开(公告)日：2012-12-25

申请号：US12709774

申请日：2010-02-22

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： G01N33/48

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US20110244469A1

公开(公告)日：2011-10-06

申请号：US13161788

申请日：2011-06-16

申请人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

发明人： Kak Namkoong ,  Jin-tae Kim ,  Young-sun Lee ,  Young-a Kim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851

摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。

公开(公告)号：US20060246580A1

公开(公告)日：2006-11-02

申请号：US11377793

申请日：2006-03-16

申请人： Jin-tae Kim ,  Kak Namkoong ,  Kwang-wook Oh ,  Chi-sung Park ,  Yu-jin Seo

发明人： Jin-tae Kim ,  Kak Namkoong ,  Kwang-wook Oh ,  Chi-sung Park ,  Yu-jin Seo

IPC分类号： C12M1/34

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L9/52 ,  B01L2300/0822 ,  B01L2300/1827 ,  B01L2300/1844 ,  C12Q1/686

摘要： Provided are a polymerase chain reaction (PCR) module and a PCR system including the same. The PCR module includes: a detachable PCR chip including a PCR chamber unit in which a PCR solution is accommodated; a heater unit for heating the PCR solution in the PCR chip with a preset temperature; a detecting unit for detecting a PCR signal of the PCR solution; a PCR chip installation unit for mounting/detaching the PCR chip using a one-touch method, in which the heater unit is adhered to the PCR chip with a predetermined pressure when mounting the PCR chip and the heater unit is separated from the PCR chip when detaching the PCR chip; and a housing covering at least the heater unit and the detecting unit so that they are not exposed to the outside.

摘要翻译： 提供了聚合酶链反应（PCR）模块和包括其的PCR系统。 所述PCR模块包括：可拆卸PCR芯片，其包括其中容纳PCR溶液的PCR室单元; 用于以预设温度加热PCR芯片中的PCR溶液的加热器单元; 用于检测PCR溶液的PCR信号的检测单元; 用于使用一触式方法安装/分离PCR芯片的PCR芯片安装单元，其中当将PCR芯片和加热器单元安装在PCR芯片上时，将加热器单元以预定的压力粘附到PCR芯片上，与PCR芯片分离， 分离PCR芯片; 以及至少覆盖加热器单元和检测单元的壳体，使得它们不暴露于外部。