Taxus transformation transformed cells, and related compositions and methods
    11.
    发明授权
    Taxus transformation transformed cells, and related compositions and methods 有权
    红豆杉转化转化细胞及相关成分和方法

    公开(公告)号:US08053637B2

    公开(公告)日:2011-11-08

    申请号:US12160066

    申请日:2007-01-03

    CPC分类号: C12N15/8205

    摘要: Reported herein are methods for obtaining stably transformed callus in Taxus media ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.

    摘要翻译: 本文报道的是在紫杉属培养基“Hicksii”中获得稳定转化的愈伤组织的方法,特别涉及使用针,茎或树皮剥离作为转化的外植体材料的方法。 还提供了在Taxus培养基细胞,特别是悬浮培养物,愈伤组织和针头细胞中指导报道基因表达的若干启动子活性的描述。 还提供转基因植物(例如紫杉属植物),植物细胞,细胞系和组织(包括种子),特别是在紫杉醇生物合成途径中表达一种或多种酶的那些。