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    Raspberry promoter and methods for expression of transgenes in plants
    2.
    发明授权
    Raspberry promoter and methods for expression of transgenes in plants 失效
    覆盆子启动子和转基因在植物中表达的方法

    公开(公告)号：US6054635A

    公开(公告)日：2000-04-25

    申请号：US777147

    申请日：1996-12-27

    申请人： Richard K. Bestwick ,  Jill A. Kellogg ,  Helena V. Mathews

    发明人： Richard K. Bestwick ,  Jill A. Kellogg ,  Helena V. Mathews

    IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

    CPC分类号： C12N15/8216 ,  C12N15/8235

    摘要： The present invention is directed to the identification and isolation of a promoter region from a raspberry genome. The promoter is operably linked, in a native raspberry genome, to the coding region of a raspberry E4 gene. The raspberry E4 gene promoter of the invention is capable of regulating moderate level, constitutive expression of a heterologous plant gene under its control. The invention is further directed to chimeric genes, cassette vectors, kits, transgenic plants, and methods employing a raspberry E4 promoter.

    摘要翻译： 本发明涉及从覆盆子基因组鉴定和分离启动子区。 启动子在天然覆盆子基因组中可操作地连接到覆盆子E4基因的编码区。 本发明的覆盆子E4基因启动子能够调节其控制下的异源植物基因的中等程度的组成型表达。 本发明还涉及嵌合基因，盒载体，试剂盒，转基因植物和使用覆盆子E4启动子的方法。

    Methods for creating color variation in anthocyanins produced by cell culture
    4.
    发明授权
    Methods for creating color variation in anthocyanins produced by cell culture 有权
    通过细胞培养产生的花青素产生颜色变异的方法

    公开(公告)号：US08962327B2

    公开(公告)日：2015-02-24

    申请号：US13260667

    申请日：2009-05-04

    申请人： Sung-Yong Yoon ,  Helena V. Mathews

    发明人： Sung-Yong Yoon ,  Helena V. Mathews

    IPC分类号： C12N5/04 ,  A01H4/00

    CPC分类号： C12N5/04 ,  A01H4/00

    摘要： Disclosed are methods for obtaining blue anthocyanin pigments and compositions including such pigments. This blue anthocyanin is present at an acidic pH (where anthocyanins are most stable) and may be used as a natural color additive. The methods can include cultivating a plant callus from a plant capable of producing anthocyanin in which the callus is treated with a blue anthocyanin-generating agent at a concentration sufficient to generate callus with blue anthocyanin pigments. The method can also include recovering the blue anthocyanin pigments from the culture. In an example, the blue anthocyanin-generating agent is ammonium molybdate.

    摘要翻译： 公开了获得蓝花青素颜料的方法和包括这种颜料的组合物。 这种蓝色花青素存在于酸性pH（其中花青素最稳定），并且可以用作天然色素。 所述方法可以包括从能够产生花青素的植物培养植物愈伤组织，其中用足够产生具有蓝色花青素色素的愈伤组织的浓度的蓝色花青素生成剂处理愈伤组织。 该方法还可以包括从培养物中回收蓝色花青素颜料。 在一个实例中，蓝色花青素生成剂是钼酸铵。

    Plant cell culture for production of natural products with reduced glucosinolate contamination
    5.
    发明授权
    Plant cell culture for production of natural products with reduced glucosinolate contamination 失效
    用于生产具有降低的硫代葡萄糖苷污染的天然产物的植物细胞培养物

    公开(公告)号：US08642340B2

    公开(公告)日：2014-02-04

    申请号：US13140381

    申请日：2009-12-17

    申请人： Helena V. Mathews ,  Sung-Yong Yoon ,  Mylavarapu Venkatramesh

    发明人： Helena V. Mathews ,  Sung-Yong Yoon ,  Mylavarapu Venkatramesh

    IPC分类号： C12N5/00

    CPC分类号： C12P17/06 ,  A01H4/00 ,  C12N5/04

    摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.

    摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。

    PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION
    6.
    发明申请
    PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED GLUCOSINOLATE CONTAMINATION 失效
    用于生产具有降低的葡萄糖苷酸盐污染的天然产品的植物细胞培养物

    公开(公告)号：US20110251408A1

    公开(公告)日：2011-10-13

    申请号：US13140381

    申请日：2009-12-17

    申请人： Helena V. Mathews ,  Sung-Yong Yoon ,  Mylavarapu Venkatramesh

    发明人： Helena V. Mathews ,  Sung-Yong Yoon ,  Mylavarapu Venkatramesh

    IPC分类号： C07D311/60 ,  C12P17/06

    CPC分类号： C12P17/06 ,  A01H4/00 ,  C12N5/04

    摘要： Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.

    摘要翻译： 本发明公开了一种从甘蓝科植物获得具有降低的硫代葡萄糖苷污染的天然产物制剂的方法。 所述方法可以包括从能够产生所需天然产物的植物培养植物愈伤组织，选择具有降低的硫代葡萄糖苷产生的愈伤组织，以及在液体培养基中培养所选择的愈伤组织。 该方法还可以包括从培养物中回收天然产物。 还公开了获得具有降低的硫代葡萄糖苷污染的甘蓝花青素的方法。 该方法可以包括在液体培养基中培养具有降低的硫代葡萄糖苷产量的红甘蓝植物愈伤组织以获得悬浮培养物，并在不含氮源的培养基中培养悬浮培养物。 该方法还可以包括从培养物中降低芥子油苷污染来回收花青素。 最后，描述了几种特异性低硫代葡萄糖苷酸细胞系。

    Taxus Transformation Transformed Cells, and Related Compositions and Methods
    7.
    发明申请
    Taxus Transformation Transformed Cells, and Related Compositions and Methods 有权
    红豆杉转化转化细胞及相关成分和方法

    公开(公告)号：US20090007298A1

    公开(公告)日：2009-01-01

    申请号：US12160066

    申请日：2007-01-03

    申请人： Helena V. Mathews ,  Vaka S. Reddy ,  Allan H. Lammers

    发明人： Helena V. Mathews ,  Vaka S. Reddy ,  Allan H. Lammers

    IPC分类号： C12N15/82 ,  C12Q1/68 ,  C12N5/04 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N15/8205

    摘要： Reported herein are methods for obtaining stably transformed callus in Taxus media ‘Hicksii’, including particularly methods that involve using needles, stem, or bark peel as explant material for transformation. Also provided are descriptions of several promoter activities in directing reporter gene expression in Taxus media cells, in particular cells in suspension cultures, callus and needles. Transgenic plants (e.g., Taxus plants), plant cells, cell lines, and tissues (including seeds) are also provided, in particular those that express one or more enzymes in a paclitaxel biosynthesis pathway.

    摘要翻译： 本文报道的是在紫杉属培养基“Hicksii”中获得稳定转化的愈伤组织的方法，特别涉及使用针，茎或树皮剥离作为转化的外植体材料的方法。 还提供了在Taxus培养基细胞，特别是悬浮培养物，愈伤组织和针头细胞中指导报道基因表达的若干启动子活性的描述。 还提供转基因植物（例如紫杉属植物），植物细胞，细胞系和组织（包括种子），特别是在紫杉醇生物合成途径中表达一种或多种酶的那些。