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公开(公告)号：US06624145B1
公开(公告)日：2003-09-23
申请号：US09547621
申请日：2000-04-12
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy A. Cardineau , George E. Schwab , Tracy Ellis Michaels
IPC分类号： A01N3718
CPC分类号： C07K14/325 , C12N2799/026
摘要： The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encoded these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.
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公开(公告)号：US6083499A
公开(公告)日：2000-07-04
申请号：US633993
申请日：1996-04-19
申请人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy Cardineau , George E. Schwab
发明人： Kenneth E. Narva , H. Ernest Schnepf , Mark Knuth , Michael R. Pollard , Guy Cardineau , George E. Schwab
IPC分类号： C12N15/09 , A01N37/18 , A01N63/00 , A01N63/02 , C07K14/325 , C12N1/20 , C12N1/21 , C12N5/04 , C12N5/10 , C12N15/00 , C12N15/32 , C12N15/82 , C12Q1/68 , C12R1/07
CPC分类号： C07K14/325 , C12N2799/026
摘要： The subject invention concerns new classes of pesticidal toxins and the polynucleotide sequences which encode these toxins. Also described are novel pesticidal isolates of Bacillus thuringiensis.
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13.发明授权
公开(公告)号：US5430137A
公开(公告)日：1995-07-04
申请号：US968781
申请日：1992-10-30
申请人： Frank H. Gaertner , August J. Sick , Mark Thompson , H. Ernest Schnepf , George E. Schwab , Kenneth E. Narva
发明人： Frank H. Gaertner , August J. Sick , Mark Thompson , H. Ernest Schnepf , George E. Schwab , Kenneth E. Narva
CPC分类号： C12Q1/689
摘要： This invention concerns novel gene probes which can be used to identify DNA from Bacillus thuringiensis microbes which encode protein endotoxins. The invention probes greatly facilitate the search for useful microbes hosting genes which encode toxins.
摘要翻译： 本发明涉及可用于鉴定编码蛋白质内毒素的苏云金芽孢杆菌微生物DNA的新型基因探针。 本发明探针极大地促进了寻找编码毒素的基因的有用微生物。
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公开(公告)号：US5985267A
公开(公告)日：1999-11-16
申请号：US962190
申请日：1997-10-31
IPC分类号： C12N15/09 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/19 , A01N63/00
CPC分类号： C12R1/075 , C07K14/325
摘要： Disclosed and claimed are novel Bacillus thuringiensis isolates which have lepidopteran activity. Thus, these isolates, or mutants thereof, can be used to control such insect pests. Further, genes encoding novel .delta.-endotoxins can be removed from the isolates and transferred to other host microbes, or plants. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
摘要翻译： 公开并声称具有鳞翅目昆虫活性的新型苏云金芽孢杆菌分离株。 因此，这些分离物或其突变体可以用于控制这种害虫。 此外，编码新的δ-内毒素的基因可以从分离物中去除并转移到其他宿主微生物或植物中。 这种宿主中的δ-内毒素的表达导致对这种宿主环境中易感害虫的控制。
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15.发明授权Genes encoding nematode-active toxins from Bacillus thuringiensis strains 失效编码来自苏云金芽孢杆菌菌株的线虫活性毒素的基因
公开(公告)号：US5753492A
公开(公告)日：1998-05-19
申请号：US316301
申请日：1994-09-30
IPC分类号： A01N37/46 , A01N63/00 , A01N63/02 , C07K14/325 , C12N15/32
CPC分类号： C07K14/325 , A01N37/46 , A01N63/00 , A01N63/02 , C12R1/075
摘要： This invention concerns genes or gene fragments which have been cloned from novel Bacillus thuringiensis isolates which have nematicidal activity. These genes or gene fragments can be used to transform suitable hosts for controlling nematodes.
摘要翻译： 本发明涉及从具有杀线虫活性的新型苏云金芽孢杆菌分离株中克隆的基因或基因片段。 这些基因或基因片段可用于转化合适的宿主用于控制线虫。
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公开(公告)号：US5686069A
公开(公告)日：1997-11-11
申请号：US291368
申请日：1994-08-15
IPC分类号： C12N15/09 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/00 , C12N15/32 , C12P21/02 , C12R1/07 , C12R1/19 , C12N1/201
CPC分类号： C12R1/075 , C07K14/325
摘要： Disclosed and claimed are novel Bacillus thuringiensis isolates which have lepidopteran activity. Thus, these isolates, or mutants thereof, can be used to control such insect pests. Further, genes encoding novel .delta.-endotoxins can be removed from the isolates and transferred to other host microbes, or plants. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.
摘要翻译： 公开并声称具有鳞翅目昆虫活性的新型苏云金芽孢杆菌分离株。 因此，这些分离物或其突变体可以用于控制这种害虫。 此外，编码新的δ-内毒素的基因可以从分离物中去除并转移到其他宿主微生物或植物中。 这种宿主中的δ-内毒素的表达导致对这种宿主环境中易感害虫的控制。
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公开(公告)号：US06632792B2
公开(公告)日：2003-10-14
申请号：US09738363
申请日：2000-12-15
IPC分类号： C07K14325
CPC分类号： C07K14/325 , A01N37/46 , A01N63/00 , A01N63/02 , C12R1/075
摘要： This invention concerns nematicidal proteins obtainable from Bacillus thuringiensis isolates. The subject invention also provides various methods of using these proteins for controlling nematodes.
摘要翻译： 本发明涉及从
苏云金芽孢杆菌 分离株获得的杀线虫蛋白。 本发明还提供了使用这些蛋白质来控制线虫的各种方法 -
公开(公告)号：US6166195A
公开(公告)日：2000-12-26
申请号：US76137
申请日：1998-05-12
CPC分类号： C07K14/325 , A01N37/46 , A01N63/00 , A01N63/02 , C12R1/075
摘要： This invention concerns genes or gene fragments which have been cloned from novel Bacillus thuringiensis isolates which have nematicidal activity. These genes or gene fragments can be used to transform suitable hosts for controlling nematodes.
摘要翻译： 本发明涉及从具有杀线虫活性的新型苏云金芽孢杆菌分离株中克隆的基因或基因片段。 这些基因或基因片段可用于转化合适的宿主用于控制线虫。
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公开(公告)号：US06752992B2
公开(公告)日：2004-06-22
申请号：US10099285
申请日：2002-03-15
申请人： H. Ernest Schnepf , Carol Wicker , Kenneth E. Narva , Michele Walz , Brian A. Stockhoff , Judy Muller-Cohn
发明人： H. Ernest Schnepf , Carol Wicker , Kenneth E. Narva , Michele Walz , Brian A. Stockhoff , Judy Muller-Cohn
IPC分类号： A61K3907
CPC分类号： C12N15/8286 , C07K14/325 , C07K2319/00 , C12N2799/026 , Y02A40/162
摘要： The subject invention concerns materials and methods useful in the control of non-mammalian pests and, particularly, plant pests. In a specific embodiment, the subject invention provides new Bacillus thuringiensis toxins useful for the control of lepidopterans. The subject invention further provides nucleotide sequences which encode the toxins of the subject invention. The nucleotide sequences of the subject invention can be used to transform hosts, such as plants, to express the pesticidal toxins of the subject invention. The subject invention further concerns novel nucleotide primers for the identification of genes encoding toxins active against pests. The primers are useful in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins. The primers are also useful as nucleotide probes to detect the toxin-encoding genes.
摘要翻译： 本发明涉及可用于控制非哺乳动物害虫，特别是植物害虫的材料和方法。 在具体实施方案中，本发明提供了可用于控制鳞翅目昆虫的新的苏云金芽孢杆菌毒素。 本发明进一步提供编码本发明毒素的核苷酸序列。 本发明的核苷酸序列可用于转化宿主如植物，以表达本发明的杀虫毒素。 本发明还涉及用于鉴定编码针对害虫的毒素的基因的新型核苷酸引物。 引物可用于PCR技术以产生编码这些毒素的基因特征的基因片段。 引物也可用作检测毒素编码基因的核苷酸探针。
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20.发明授权Polynucleotide encoding lepidopteran active toxin PS86I2 derived from Bacillus thuringiensis and methods of using PS86I2 失效编码来自苏云金芽孢杆菌的鳞翅目昆虫活性毒素PS86I2的多核苷酸和使用PS86I2的方法
公开(公告)号：US6107278A
公开(公告)日：2000-08-22
申请号：US41991
申请日：1998-03-13
IPC分类号： C12N15/09 , A01N63/00 , A01N63/02 , C07K14/325 , C12N5/10 , C12N15/32 , C07K14/00 , C07H17/00 , C12P21/06
CPC分类号： C12R1/075 , A01N63/00 , C07K14/325
摘要： The subject invention pertains to novel insecticidal toxins and genes which encode these toxins. Also disclosed are novel nucleotide primers for the identification of genes encoding toxins active against pests. The primers are usefull in PCR techniques to produce gene fragments which are characteristic of genes encoding these toxins.
摘要翻译： 本发明涉及编码这些毒素的新型杀虫毒素和基因。 还公开了用于鉴定编码针对害虫的毒素的基因的新型核苷酸引物。 引物在PCR技术中可用于产生基因片段，其是编码这些毒素的基因的特征。
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