公开(公告)号：US11016083B2

公开(公告)日：2021-05-25

申请号：US16071716

申请日：2017-01-30

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC分类号： C12N15/09 ,  C12Q1/6897 ,  G01N33/50 ,  C12N15/10

摘要： The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.

公开(公告)号：US20190048317A1

公开(公告)日：2019-02-14

申请号：US15735685

申请日：2016-06-16

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Sou Nakamura ,  Yukitaka Ito ,  Tomohiro Shigemori ,  Takeaki Dohda

IPC分类号： C12N5/078 ,  A61K31/553 ,  A61K35/19 ,  A61K31/551 ,  C12N5/077

摘要： The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors.

公开(公告)号：US11977071B2

公开(公告)日：2024-05-07

申请号：US17216926

申请日：2021-03-30

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/6897 ,  G01N33/50

CPC分类号： G01N33/5023 ,  C12N15/09 ,  C12N15/1086 ,  C12Q1/6897 ,  G01N33/5044

摘要： The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.

公开(公告)号：US11952587B2

公开(公告)日：2024-04-09

申请号：US16491219

申请日：2018-03-05

申请人： KYOTO UNIVERSITY ,  MEGAKARYON CORPORATION

发明人： Koji Eto ,  Sou Nakamura ,  Yukitaka Ito

IPC分类号： C12N5/078 ,  C12N15/85

CPC分类号： C12N5/0644 ,  C12N15/85 ,  C12N2501/105 ,  C12N2506/02

摘要： Healthy functional platelets are mass produced. A method for producing platelets, comprising: (1) a culture step of culturing megakaryocytes in a platelet producing medium in the presence of mechanical stress and platelet production promoting factors including MIF, NRDc, IGFBP2, TSP-1, PAI-1, and CCL5, and (2) a harvest step of harvesting the platelets obtained by the culture step; wherein the culture step comprises: (a) a step of promoting a release of the platelet production promoting factors from megakaryocytes by mechanical stress; and/or (b) a step of externally adding platelet production promoting factors including MIF, NRDc, and IGFBP2.

公开(公告)号：US20210239682A1

公开(公告)日：2021-08-05

申请号：US17216926

申请日：2021-03-30

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/6897 ,  C12N15/10

摘要： The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.

公开(公告)号：US10947504B2

公开(公告)日：2021-03-16

申请号：US15735685

申请日：2016-06-16

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Sou Nakamura ,  Yukitaka Ito ,  Tomohiro Shigemori ,  Takeaki Dohda

IPC分类号： C12N5/078 ,  A61K31/553 ,  A61K35/19 ,  A61K31/551 ,  C12N5/077 ,  C12N15/113 ,  C12N15/09

摘要： The present invention provides a highly functional platelet production promoting agent which comprises one or a plurality of aryl hydrocarbon receptor (AhR) antagonists and one or a plurality of Rho-associated coiled-coil forming kinase (ROCK) inhibitors.

公开(公告)号：US20190033296A1

公开(公告)日：2019-01-31

申请号：US16071716

申请日：2017-01-30

申请人： Kyoto University ,  Megakaryon Corporation

发明人： Koji Eto ,  Hideya Seo ,  Akira Ota ,  Yukitaka Ito ,  Yasuko Hazama

IPC分类号： G01N33/50 ,  C12Q1/6897

摘要： The present invention provides a method for screening for platelet production promoters, the method including a step for selecting a candidate substance that significantly increases expression of TUBB1 as a platelet production promoter.

公开(公告)号：US20230030814A1

公开(公告)日：2023-02-02

申请号：US17783097

申请日：2020-12-11

申请人： National University Corporation Chiba University ,  Kyoto University

发明人： Seiji Ohtori ,  Yasuhiro Shiga ,  Kentaro Kosaka ,  Koji Eto

IPC分类号： A61K35/19 ,  A61K9/19 ,  A61P19/00 ,  A61P17/02 ,  A61K38/18

摘要： The problem that differences among lots in components and amounts of growth factors and the like in platelet-rich plasmas derived from an autologous plasma and the freeze-dried preparations thereof are large, and the effects of the lots are not kept constant is solved.
Specifically, a freeze-dried preparation comprising megakaryocytes and platelets induced to differentiate from stem cells, a pharmaceutical composition comprising the freeze-dried preparation, and the like are provided.