公开(公告)号：US10902593B2

公开(公告)日：2021-01-26

申请号：US16557376

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey Marks ,  David Woo ,  Wallace George

IPC: G06T7/00 ,  H04N5/372 ,  H04N5/347 ,  G01N27/447

Abstract: A biological analysis system and an associated method are provided. The method is used for recovering off scale data in an image produced by a camera in a capillary electrophoresis instrument. The method comprises the steps of identifying bins of the image where electron counts exceed a maximum number of counts; setting an off-scale flag for the identified bins; and processing the image to obtain a recovered dye signal, based on the flag set for each bin, and using a dye matrix.

公开(公告)号：US10012590B2

公开(公告)日：2018-07-03

申请号：US15017249

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Thomas Wessel ,  David Woo

IPC: H01J40/14 ,  G01N21/64 ,  C12Q1/686 ,  G01N21/27 ,  C12Q1/6851

CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

公开(公告)号：US20160313280A1

公开(公告)日：2016-10-27

申请号：US15130532

申请日：2016-04-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David A. Denny ,  David Woo ,  Manjula Aliminati ,  Siva Kumar Samsani ,  Stephanie J. Schneider ,  Yoke Peng Lim ,  Sylvia Chang

IPC: G01N27/447

CPC classification number: G01N27/44717 ,  G06F19/22

Abstract: In one exemplary embodiment, a method for detecting variants in electropherogram data is provided. The method includes receiving electropherogram data from an instrument and analyzing the electropherogram data to identify mixed bases in the electropherogram data. The method further includes identifying features within the electropherogram data indicative of errors and validating the identified mixed bases. Then the method includes determining variants in the electropherogram data based on the validated mixed bases.

Abstract translation: 在一个示例性实施例中，提供了一种用于检测电泳图数据中的变体的方法。 该方法包括从仪器接收电泳图数据，并分析电泳图数据以识别电泳图数据中的混合碱基。 该方法还包括识别电泳图中指示错误并验证所识别的混合碱基的特征。 然后该方法包括基于经验证的混合碱基来确定电泳图数据中的变体。

公开(公告)号：US20160231246A1

公开(公告)日：2016-08-11

申请号：US15017249

申请日：2016-02-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yong Chu ,  Jeffrey Marks ,  Jacob Freudenthal ,  Thomas Wessel ,  David Woo

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6428 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/274 ,  G01N21/278 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2021/6471 ,  G01N2201/127 ,  G01N2201/13 ,  G01N2333/922

Abstract: In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. The method includes performing a region-of-interest (ROI) calibration to determine reaction site positions in an image. The method further includes performing a pure dye calibration to determine the contribution of a fluorescent dye used in each reaction site by comparing a raw spectrum of the fluorescent dye to a pure spectrum calibration data of the fluorescent dye. The method further includes performing an instrument normalization calibration to determine a filter normalization factor. The method includes performing an RNase P validation to validate the instrument is capable of distinguishing between two different quantities of sample.

Abstract translation: 在一个示例性实施例中，提供了一种用于校准仪器的方法。 该仪器包括能够成像来自多个反应位点的荧光发射的光学系统。 该方法包括执行感兴趣区域（ROI）校准以确定图像中的反应位置位置。 该方法还包括进行纯染料校准，以通过将荧光染料的原始光谱与荧光染料的纯光谱校准数据进行比较来确定每个反应位点中使用的荧光染料的贡献。 该方法还包括执行仪器归一化校准以确定滤波器归一化因子。 该方法包括执行RNase P验证以验证仪器能够区分两种不同数量的样品。