公开(公告)号：US11466315B2

公开(公告)日：2022-10-11

申请号：US16713491

申请日：2019-12-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dennis Wang ,  Lori Hennessy

IPC: C12Q1/6858 ,  C12Q1/686

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

公开(公告)号：US20200255892A1

公开(公告)日：2020-08-13

申请号：US16799810

申请日：2020-02-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina Bormann Chung ,  Julio Mulero ,  Lori Hennessy

IPC: C12Q1/6858 ,  C12Q1/6879 ,  C12Q1/6888

Abstract: Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.

公开(公告)号：US10519491B2

公开(公告)日：2019-12-31

申请号：US15367241

申请日：2016-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dennis Wang ,  Lori Hennessy

IPC: C12Q1/6858 ,  C12Q1/686

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

公开(公告)号：US10381111B2

公开(公告)日：2019-08-13

申请号：US15432915

申请日：2017-02-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael Harrold ,  Lori Hennessy ,  Leonard Klevan

IPC: G16H10/40 ,  G16H10/60 ,  G06F16/245 ,  A61B10/00 ,  A61B5/1172 ,  A61B5/1174 ,  A61B10/02 ,  C12Q1/6806 ,  G06K9/00 ,  C12Q1/6881 ,  G06F19/00

Abstract: Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.

公开(公告)号：US20180018422A1

公开(公告)日：2018-01-18

申请号：US15667236

申请日：2017-08-02

Applicant: Life Technologies Corporation

Inventor： Robert LAGACE ,  Chien-Wei Chang ,  Lori Hennessy

IPC: G06F19/14 ,  G06F19/18

CPC classification number: G16B10/00 ,  G16B20/00

Abstract: Systems and methods for calculating a predictive index of identity of a nucleic acid sample using polymorphic genetic marker data are provided. In one embodiment, a predictive index of identity of the nucleic acid sample is calculated using a value from a second set of data from a polymorphic genetic marker that is not linked to a polymorphic genetic marker used to produce a first set of data. In another embodiment, the predictive index of identity is calculated using a value from a second set of data from a polymorphic genetic marker that is linked to a polymorphic genetic marker used to produce the first set of data. Systems and methods for generating an identifier for a biological sample and for verifying a relationship between a biological sample and an identifier are also provided. The identifier is an encoding of a set of values for polymorphic genetic markers.

公开(公告)号：US20170081715A1

公开(公告)日：2017-03-23

申请号：US15367241

申请日：2016-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DENNIS WANG ,  Lori Hennessy

IPC: C12Q1/68

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

公开(公告)号：US20140141422A1

公开(公告)日：2014-05-22

申请号：US14044731

申请日：2013-10-02

Applicant: Life Technologies Corporation

Inventor： Dennis Wang ,  Lori Hennessy

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/151 ,  C12Q2527/107 ,  C12Q2527/113

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

Abstract translation: 公开了扩增核酸序列的方法，其中所述方法包括使反应混合物进行至少一个扩增循环，其中所述反应混合物包含双链核酸和至少两个能够退火至所述互补链的引物 双链核酸，并且在25秒或更短时间内具有两步扩增循环的Fast PCR方案中使用Family A DNA聚合酶扩增至少一个短串联重复（STR）。 还公开了使用使用Fast PCR方案的STR分析的两步方案和试剂盒的实时PCR方法。