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    Fast PCR for STR genotyping
    1.
    发明授权
    Fast PCR for STR genotyping 有权

    公开(公告)号：US11466315B2

    公开(公告)日：2022-10-11

    申请号：US16713491

    申请日：2019-12-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Dennis Wang ,  Lori Hennessy

    IPC: C12Q1/6858 ,  C12Q1/686

    Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

    Fast PCR for STR genotyping
    2.
    发明授权
    Fast PCR for STR genotyping 有权

    公开(公告)号：US10519491B2

    公开(公告)日：2019-12-31

    申请号：US15367241

    申请日：2016-12-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Dennis Wang ,  Lori Hennessy

    IPC: C12Q1/6858 ,  C12Q1/686

    Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

    Fast PCR for STR Genotyping
    3.
    发明申请
    Fast PCR for STR Genotyping 审中-公开
    STR基因分型的快速PCR

    公开(公告)号：US20140141422A1

    公开(公告)日：2014-05-22

    申请号：US14044731

    申请日：2013-10-02

    Applicant: Life Technologies Corporation

    Inventor： Dennis Wang ,  Lori Hennessy

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/151 ,  C12Q2527/107 ,  C12Q2527/113

    Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

    Abstract translation: 公开了扩增核酸序列的方法，其中所述方法包括使反应混合物进行至少一个扩增循环，其中所述反应混合物包含双链核酸和至少两个能够退火至所述互补链的引物 双链核酸，并且在25秒或更短时间内具有两步扩增循环的Fast PCR方案中使用Family A DNA聚合酶扩增至少一个短串联重复（STR）。 还公开了使用使用Fast PCR方案的STR分析的两步方案和试剂盒的实时PCR方法。

    SE33 MUTATIONS IMPACTING GENOTYPE CONCORDANCE
    10.
    发明申请
    SE33 MUTATIONS IMPACTING GENOTYPE CONCORDANCE 审中-公开
    SE33突变影响基因型的意义

    公开(公告)号：US20160010146A1

    公开(公告)日：2016-01-14

    申请号：US14809418

    申请日：2015-07-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Robert GREEN ,  Dennis Wang ,  Julio Mulero ,  Robert Lagace

    IPC: C12Q1/68

    CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6888 ,  C12Q2537/143 ,  C12Q2600/156

    Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

    Abstract translation: 公开了使用高度复杂的序列位点SE33（ACTBP2）在单次和多重PCR反应中进行人类鉴定的引物组合物，方法和试剂盒。 另外，所公开的是在SE33基因座内的三种新发现的单核苷酸多态性（SNP），其可以导致视为移动性偏移或等位基因缺失的不一致。 还公开了可用于人类鉴定的试剂盒。

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