公开(公告)号：US20150267264A1

公开(公告)日：2015-09-24

申请号：US14624185

申请日：2015-02-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Rixun FANG ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

Abstract translation: 本文提供的方法和组合物涉及在人类Y染色体上发现的13种STR标记，与目前已知的173种其它Y-STR标记物相比具有惊人的高突变率。 RM-Y-STR的集合可以克服由于其非凡的突变特性而导致的法医应用中Y染色体分析目前的困境。 本发明的实施方案包括用于等位基因测定快速突变的Y-STR标记的方法，用于分析快速突变的Y-STR标记的扩增引物，用于分析快速突变Y-STR标记的等位基因梯度和用于分析的试剂盒 的快速突变的Y-STR标记。

公开(公告)号：US20130236896A1

公开(公告)日：2013-09-12

申请号：US13870779

申请日：2013-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Paolo VATTA ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而非大肠杆菌O55：H7的分析，方法和试剂盒，来自复杂的食物基质，水，饮料样品，发酵液，法医样品，环境样品（例如 ，土壤，污垢，垃圾，污水，空气或水），包括食品加工和制造表面，或生物样品。