公开(公告)号：US20160348092A1

公开(公告)日：2016-12-01

申请号：US15235327

申请日：2016-08-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

Abstract translation: 本教导涉及从固体材料中提取核酸。 提供了用于从固体生物样品获得核酸的有用的组合物，方法和试剂盒或具有粘附或嵌入粘合剂基底内的生物材料的粘合剂材料。 提取的核酸可用于下游应用，例如生物材料来源的基因分型，检测，定量和鉴定。

公开(公告)号：US09422544B2

公开(公告)日：2016-08-23

申请号：US13931110

申请日：2013-06-28

Applicant: Life Technologies Corporation

Inventor： Maxim Brevnov ,  Hemant Pawar ,  Manohar Furtado ,  Jaiprakash Shewale

IPC: C07H21/02 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.