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    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS
    4.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
    用于检测多种微生物的组合物和方法

    公开(公告)号：US20140005061A1

    公开(公告)日：2014-01-02

    申请号：US13790447

    申请日：2013-03-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Robert TEBBS ,  Craig Cummings ,  Pius Brzoska ,  Sharon Matheny ,  Catherine O'Connell ,  Manohar Furtado ,  Nikolay Sergeev ,  Maxim Brevnov

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

    Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

    METHODS FOR DETECTION OF MULTIPLE TARGET NUCLEIC ACIDS
    6.
    发明申请
    METHODS FOR DETECTION OF MULTIPLE TARGET NUCLEIC ACIDS 审中-公开
    用于检测多种目标核酸的方法

    公开(公告)号：US20140272940A1

    公开(公告)日：2014-09-18

    申请号：US14206716

    申请日：2014-03-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wayne Ge ,  Craig Cummings ,  Darcy Myers ,  Quoc Hoang ,  Catherine O'Connell ,  Maxim Brevnov ,  Daniel Kephart

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6809 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2531/113

    Abstract: The present disclosure, in some embodiments, relates to compositions and methods for detection of multiple target nucleic acid sequences by a single nucleic acid amplification based assay (e.g. PCR). Compositions comprising two or more primers are described for multiple target nucleic acid detection. Methods for differential detection of microorganisms (including strains/serovars/subtypes thereof) and cell types comprise a single step method to detect a signature of target nucleic acid sequences comprising: two, three or more target nucleic acids that are uniquely present in a microorganism/strain/serovar/subtype and/or cell and absent in other closely related organisms/cells. Embodiments relate to methods of diagnosis of diseases or conditions that can be detected by detecting the presence of two or more nucleic acid target markers.

    Abstract translation: 在一些实施方案中，本公开涉及通过基于单个核酸扩增的测定（例如PCR）检测多个靶核酸序列的组合物和方法。 描述了包含两个或多个引物的组合物用于多靶核酸检测。 用于差异检测微生物（包括菌株/血清型/亚型）和细胞类型的方法包括检测靶核酸序列的特征的单步方法，其包括：两个或三个以上唯一存在于微生物/ 菌株/血清型/亚型和/或细胞，并且在其他紧密相关的生物/细胞中不存在。 实施方案涉及通过检测两种或多种核酸靶标志物的存在可以检测的疾病或病症的诊断方法。

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