公开(公告)号：US20050266433A1

公开(公告)日：2005-12-01

申请号：US11071679

申请日：2005-03-03

申请人： Ravi Kapur ,  Mehmet Toner ,  Bruce Carvalho ,  Tom Barber ,  Lotien Huang

发明人： Ravi Kapur ,  Mehmet Toner ,  Bruce Carvalho ,  Tom Barber ,  Lotien Huang

IPC分类号： B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54326 ,  B01L3/5027

摘要： The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.

摘要翻译： 本发明的特征在于一种新的有用的磁性装置及其用于细胞，蛋白质，DNA和其他分子的分离，富集和纯化的方法。 通常，该装置包括磁性颗粒可以结合的磁性区域或障碍物。 然后可以将磁性颗粒表面上的化学基团，即捕获部分用于结合来自复杂样品的颗粒，例如细胞或感兴趣的分子，然后可以选择性地释放结合的物质用于下游收集或进一步 分析。

公开(公告)号：US20050161331A1

公开(公告)日：2005-07-28

申请号：US11075682

申请日：2005-03-09

申请人： Lotien Huang ,  James Sturm ,  Robert Austin

发明人： Lotien Huang ,  James Sturm ,  Robert Austin

IPC分类号： C07H21/04 ,  B01L3/00 ,  C12N15/10 ,  G01N27/447 ,  G01N27/453

CPC分类号： C12N15/101 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/25

摘要： A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field having two different fields at different orientations is applied. The alternating electric field is asymmetric in that one field is stronger in duration or intensity than the other field, or is otherwise asymmetric. The DNA molecules are thereby fractionated according to site and are driven to a far side of the matrix where the fractionated DNA is recovered. The fractionating electric field can be used to load and recover the DNA to operate the process continuously.