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1.发明授权Devices and method for enrichment and alteration of cells and other particles 有权用于浓缩和改变细胞和其他颗粒的装置和方法公开(公告)号：US08585971B2
公开(公告)日：2013-11-19
申请号：US13452054
申请日：2012-04-20
Applicant: Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
Inventor： Lotien Richard Huang , Thomas A. Barber , Bruce L. Carvalho , Ravi Kapur , Paul Vernucci , Mehmet Toner , Zihua Wang
IPC: G01N33/00
CPC classification number: B03C1/30 , B01L3/502746 , B01L3/502753 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0472 , B01L2400/0487 , B01L2400/086 , B03C1/32 , B03C2201/18 , B33Y80/00 , C12M47/04 , C12M47/06 , G01N1/40 , G01N1/4077 , G01N33/5044 , G01N2035/00237 , Y10T137/0318 , Y10T137/8593 , Y10T436/25 , Y10T436/25375
Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.
Abstract translation: 本发明的特征在于确定性分离颗粒的装置和方法。 示例性的方法包括在所需颗粒中富集样品或改变装置中所需的颗粒。 该装置和方法有利地用于富集存在于样品中的稀有细胞，例如胎儿细胞，例如母体血液和稀有细胞成分，例如胎儿细胞核。 本发明还提供了用于优先裂解样品中感兴趣的细胞的方法，例如从目的细胞的细胞组分（例如细胞核）提取临床信息。 通常，该方法在样品中感兴趣的细胞和其他细胞（例如其他有核细胞）之间使用差异裂解。
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2.发明申请DETERMINATION OF FETAL ANEUPLOIDY BY QUANTIFICATION OF GENOMIC DNA FROM MIXED SAMPLES 审中-公开通过从混合样品中定量鉴定基因组DNA来确定天然异构体公开(公告)号：US20130288242A1
公开(公告)日：2013-10-31
申请号：US13738268
申请日：2013-01-10
Applicant: Roland Stoughton , Ravi Kapur , Mehmet Toner , Daniel Shoemaker , Ronald W. Davis
Inventor： Roland Stoughton , Ravi Kapur , Mehmet Toner , Daniel Shoemaker , Ronald W. Davis
IPC: C12Q1/68
CPC classification number: G01N1/30 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G16B20/00
Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.
Abstract translation: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常（即非整倍体）的系统，装置和方法。 此外，本发明提供了确定何时胎儿细胞不足以确定并报告非信息性病例的方法。 本发明涉及量化来自混合样品的基因组DNA的区域。 更具体地，本发明涉及从混合样品中定量DNA多态性。
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公开(公告)号：US20130189689A1
公开(公告)日：2013-07-25
申请号：US13837974
申请日：2013-03-15
Applicant: Daniel Shoemaker , Ravi Kapur , Mehmet Toner , Roland Stoughton , Ronald W. Davis
Inventor： Daniel Shoemaker , Ravi Kapur , Mehmet Toner , Roland Stoughton , Ronald W. Davis
IPC: C12Q1/68
CPC classification number: C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
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公开(公告)号：US08168389B2
公开(公告)日：2012-05-01
申请号：US12230628
申请日：2008-09-02
Applicant: Daniel Shoemaker , Ravi Kapur , Mehmet Toner , Roland Stoughton , Ronald W. Davis
Inventor： Daniel Shoemaker , Ravi Kapur , Mehmet Toner , Roland Stoughton , Ronald W. Davis
IPC: C12Q1/68
CPC classification number: C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
Abstract translation: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统，装置和方法，例如， 非整倍体 本发明涉及用不同的标记标记所述混合样品中每个细胞中的基因组DNA的区域，其中每个标记对每个细胞是特异性的，并且定量混合样品中每个细胞的基因组DNA的标记区域。 更具体地，本发明涉及从混合样品中的每个细胞定量标记的DNA多态性。
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公开(公告)号：US20110003293A1
公开(公告)日：2011-01-06
申请号：US12751931
申请日：2010-03-31
Applicant: Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
Inventor： Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6883 , C12Q2600/156
Abstract: The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.
Abstract translation: 本发明提供了用于从样品中富集组分或细胞并进行遗传分析的装置和方法，例如SNP基因分型以提供胎儿障碍或病症的诊断结果。
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Patent Agency Ranking
公开(公告)号：US20100291572A1
公开(公告)日：2010-11-18
申请号：US12751940
申请日：2010-03-31
Applicant: Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
Inventor： Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6883 , C12Q2600/156
Abstract: The present invention provides apparatus and methods for enriching components or cells from a sample and conducting genetic analysis, such as SNP genotyping to provide diagnostic results for fetal disorders or conditions.
Abstract translation: 本发明提供了用于从样品中富集组分或细胞并进行遗传分析的装置和方法，例如SNP基因分型以提供胎儿障碍或病症的诊断结果。
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7.发明申请DIAGNOSIS OF FETAL ANEUPLOIDY BY QUANTIFICATION OF GENOMIC DNA FROM MIXED SAMPLES 审中-公开通过从混合样品中定量鉴定基因组DNA来诊断子宫内膜异位症公开(公告)号：US20100291571A1
公开(公告)日：2010-11-18
申请号：US12751908
申请日：2010-03-31
Applicant: Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
Inventor： Roland Stoughton , Ravi Kapur , Barb Ariel Cohen
IPC: C12Q1/68
CPC classification number: G01N1/30 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G16B20/00
Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.
Abstract translation: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常（即非整倍体）的系统，装置和方法。 此外，本发明提供了确定何时胎儿细胞不足以确定并报告非信息性病例的方法。 本发明涉及量化来自混合样品的基因组DNA的区域。 更具体地，本发明涉及从混合样品中定量DNA多态性。
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8.发明申请DIAGNOSIS OF FETAL ABNORMALITIES BY COMPARATIVE GENOMIC HYBRIDIZATION ANALYSIS 审中-公开通过比较基因组杂交分析诊断不典型性公开(公告)号：US20100112586A1
公开(公告)日：2010-05-06
申请号：US12608876
申请日：2009-10-29
Applicant: Roland STOUGHTON , Ravi Kapur
Inventor： Roland STOUGHTON , Ravi Kapur
IPC: C12Q1/68
CPC classification number: C12Q1/6809 , C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , G01N2015/1006 , G01N2015/1087
Abstract: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves performing comparative genomic hybridization (CGH) analysis when fetal cells are present in a mixed population of cells. The present invention involves detecting the presence of fetal cells in a mixed maternal sample by detecting the presence of non-maternal alleles in said sample. Furthermore, the present invention also involves correlating the presence of fetal cells in a mixed sample with CGH analysis results to detect a fetal abnormality or declare a test non-informative.
Abstract translation: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统，装置和方法，例如， 非整倍体 当胎儿细胞存在于混合细胞群体中时，本发明涉及进行比较基因组杂交（CGH）分析。 本发明涉及通过检测所述样品中非母体等位基因的存在来检测混合母体样品中胎儿细胞的存在。 此外，本发明还涉及将混合样品中的胎儿细胞的存在与CGH分析结果相关联以检测胎儿异常或者宣布测试非信息。
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9.发明申请Magnetic Device for Isolation of Cells and Biomolecules in a Microfluidic Environment 审中-公开用于在微流体环境中分离细胞和生物分子的磁性装置公开(公告)号：US20100055758A1
公开(公告)日：2010-03-04
申请号：US12509237
申请日：2009-07-24
Applicant: Ravi Kapur , Mehmet Toner , Bruce L. Carvalho , Thomas J. Barber , Lotien R. Huang
Inventor： Ravi Kapur , Mehmet Toner , Bruce L. Carvalho , Thomas J. Barber , Lotien R. Huang
IPC: C12N13/00
CPC classification number: G01N33/54326 , B01L3/5027
Abstract: The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the surface of the magnetic particles may then be used to bind particles, e.g., cells, or molecules of interest from complex samples, and the bound species may then be selectively released for downstream collection or further analysis.
Abstract translation: 本发明的特征在于一种新的有用的磁性装置及其用于细胞，蛋白质，DNA和其他分子的分离，富集和纯化的方法。 通常，该装置包括磁性颗粒可以结合的磁性区域或障碍物。 然后可以将磁性颗粒表面上的化学基团，即捕获部分用于结合来自复杂样品的颗粒，例如细胞或感兴趣的分子，然后可以选择性地释放结合的物质用于下游收集或进一步 分析。
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公开(公告)号：US20080124721A1
公开(公告)日：2008-05-29
申请号：US11762750
申请日：2007-06-13
Applicant: Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
Inventor： Martin Fuchs , Ravi Kapur , Mehmet Toner , Zihua Wang
IPC: C12Q1/68 , G01N33/574 , C12M1/34
CPC classification number: C12Q1/6886 , C12Q2600/156 , G01N1/405 , G01N33/5005 , G01N33/5091 , G01N33/57484 , G01N2015/1006 , G01N2015/1087 , G01N2800/385
Abstract: The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g., epithelial cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject by analyzing a cellular sample from the subject.
Abstract translation: 本发明涉及用于检测，富集和分析存在于血液中的稀有细胞例如上皮细胞的方法。 本发明进一步具有分析稀有细胞以通过分析来自受试者的细胞样品来确定受试者中异常，疾病或病症的存在的方法。
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