公开(公告)号：US20100313292A1

公开(公告)日：2010-12-09

申请号：US12785722

申请日：2010-05-24

申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell ,  Yongzhong Wu ,  Howard Hershey

发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell ,  Yongzhong Wu ,  Howard Hershey

IPC分类号： A01H1/06

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8231 ,  C12N15/8287

摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

公开(公告)号：US20100287656A1

公开(公告)日：2010-11-11

申请号：US12786556

申请日：2010-05-25

申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell ,  Yongzhong Wu ,  Howard Hershey

发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell ,  Yongzhong Wu ,  Howard Hershey

IPC分类号： A01H1/06

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8231 ,  C12N15/8287

摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.

公开(公告)号：US20090183275A1

公开(公告)日：2009-07-16

申请号：US12400175

申请日：2009-03-09

申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N5/10 ,  A01H5/00 ,  C12N15/63

CPC分类号： C12N15/8231 ,  C07K14/415 ,  C12N15/8287 ,  C12N15/8289

摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

摘要翻译： 描述了介导植物中雄性生育力的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还鉴定了启动子序列及其必需区域。 核苷酸序列可用于介导植物中的雄性生殖力。

公开(公告)号：US20090183272A1

公开(公告)日：2009-07-16

申请号：US12400142

申请日：2009-03-09

申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N15/8231 ,  C07K14/415 ,  C12N15/8287 ,  C12N15/8289

摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

公开(公告)号：US5792853A

公开(公告)日：1998-08-11

申请号：US470354

申请日：1995-06-07

申请人： Andrew M. Cigan ,  Marc C. Albertsen

发明人： Andrew M. Cigan ,  Marc C. Albertsen

IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00 ,  C12N15/05

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

公开(公告)号：US5763243A

公开(公告)日：1998-06-09

申请号：US479382

申请日：1995-06-07

申请人： Andrew M. Cigan ,  Marc C. Albertsen

发明人： Andrew M. Cigan ,  Marc C. Albertsen

IPC分类号： A01H5/00 ,  A01H1/02 ,  A01H1/06 ,  C07K14/415 ,  C12N5/10 ,  C12N9/10 ,  C12N15/05 ,  C12N15/09 ,  C12N15/82 ,  C12P21/02 ,  C12N15/00 ,  A01H1/00 ,  A01H4/00

CPC分类号： C12N9/1007 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N15/8289 ,  Y10S47/01

摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

公开(公告)号：US5750868A

公开(公告)日：1998-05-12

申请号：US351899

申请日：1994-12-08

申请人： Andrew M. Cigan ,  Marc C. Albertsen

发明人： Andrew M. Cigan ,  Marc C. Albertsen

IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C12H15/00 ,  A01H1/06 ,  A01H4/00 ,  C12H15/05

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and structural genes capable of acting in a cascading fashion to produce a reversible effect on a plant phenotype. A first genetic construct comprising (i) an operator that is capable of controlling expression of a dominant negative gene, (ii) a dominant negative gene that, when expressed in a plant disrupts pollen formation or function, (iii) a tissue specific promoter that regulates the expression of a gene encoding a DNA-binding protein which binds to the operator and activates transcription, and (iv) a gene encoding a DNA binding region and an activating domain, causes male sterility in a plant. In particular, the present invention relates to the use of a DAM-methylase gene as a dominant negative gene and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene.

摘要翻译： 可以通过用包括调节元件和能够以级联方式起作用的结构基因的遗传构建体转化植物以对植物表型产生可逆效应来改变植物发育。 第一遗传构建体，其包含（i）能够控制显性阴性基因表达的操纵子，（ii）当在植物中表达时破坏花粉形成或功能的显性负基因，（iii）组织特异性启动子， 调节编码与操作者结合并激活转录的DNA结合蛋白的基因的表达，和（iv）编码DNA结合区和激活结构域的基因在植物中引起雄性不育。 特别地，本发明涉及DAM-甲基化酶基因作为显性阴性基因和花药特异性启动子的用途。 通过掺入抑制显性阴性基因的第二遗传构建体的植物中，雄性不育是逆转的。

公开(公告)号：US5689049A

公开(公告)日：1997-11-18

申请号：US471601

申请日：1995-06-07

申请人： Andrew M. Cigan ,  Marc C. Albertsen

发明人： Andrew M. Cigan ,  Marc C. Albertsen

IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C12N15/00 ,  A01H1/06 ,  A01H4/00 ,  C12N15/05

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant male-sterile. In particular, the present invention relates to the use of an anther-specific promoter, designated 5126, and its operable linkage to a dominant negative gene, more particularly an exogenous methylase gene or DAM methylase gene, to achieve male sterility.

摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物雄性不育。 特别地，本发明涉及使用命名为5126的花药特异性启动子及其与显性阴性基因，更特别是外源甲基化酶基因或DAM甲基化酶基因的可操作连接以实现雄性不育。

公开(公告)号：US20120005792A1

公开(公告)日：2012-01-05

申请号：US13189631

申请日：2011-07-25

申请人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

发明人： Marc C. Albertsen ,  Tim Fox ,  Gary Huffman ,  Mary Trimnell

IPC分类号： A01H5/00 ,  C12N15/113 ,  C12N15/63 ,  C12N15/29 ,  C12N5/10

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8231 ,  C12N15/8287

摘要： Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences and promoter sequences are useful in methods mediating male fertility in plants, as described. Vectors, plant cells, and plants comprising the nucleotide sequences and/or promoter regions are also provided.

摘要翻译： 描述介导植物中雄性生育力的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还鉴定了启动子序列及其必需区域。 如所述，核苷酸序列和启动子序列可用于介导植物中雄性生育力的方法。 还提供了载体，植物细胞和包含核苷酸序列和/或启动子区的植物。

公开(公告)号：US20110191878A1

公开(公告)日：2011-08-04

申请号：US13022336

申请日：2011-02-07

申请人： Marc C. Albertsen ,  Tim Fox ,  Mary Trimnell ,  Yongzhong Wu ,  Keith Lowe ,  Bailin Li ,  Marianna Faller

发明人： Marc C. Albertsen ,  Tim Fox ,  Mary Trimnell ,  Yongzhong Wu ,  Keith Lowe ,  Bailin Li ,  Marianna Faller

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/10

CPC分类号： C12N15/8231 ,  C07K14/415 ,  C12N15/8289

摘要： Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

摘要翻译： 描述了在植物中对雄性生育力至关重要的Msca1基因的核苷酸序列，其中阐述了DNA分子和氨基酸序列。 还鉴定了启动子序列及其必需区域。 核苷酸序列可用于影响植物中的雄性生殖力。