Rhamnose-inducible expression systems and methods
    11.
    发明申请
    Rhamnose-inducible expression systems and methods 有权
    鼠李糖诱导型表达系统和方法

    公开(公告)号:US20070122881A1

    公开(公告)日:2007-05-31

    申请号:US11580095

    申请日:2006-10-11

    申请人: Mark Surber

    发明人: Mark Surber

    摘要: Rhamnose-inducible expression constructs are described. The expression constructs may be either episomal or chromosomal and may include at least one rhamnose-inducible regulatory element expressing a regulatory protein and at least one promoter that is inducible by the regulatory protein. An open reading frame expressing a protein of interest may be placed under control of the promoter. Also described are optimized Shine-Dalgarno sequences for use with the promoter.

    摘要翻译: 描述了鼠李糖诱导型表达构建体。 表达构建体可以是附加型或染色体的,并且可以包括至少一种表达调节蛋白的鼠李糖诱导型调节元件和至少一种可被调节蛋白诱导的启动子。 表达目的蛋白质的开放阅读框可以置于启动子的控制之下。 还描述了与启动子一起使用的优化的亮 - 达加尔诺序列。