公开(公告)号：US6055552A

公开(公告)日：2000-04-25

申请号：US961691

申请日：1997-10-31

申请人： Bo U. Curry

发明人： Bo U. Curry

IPC分类号： G06F17/22 ,  G06F3/033 ,  G06F3/041 ,  G06F3/048 ,  G06F17/24 ,  G06T11/80 ,  G06F17/30

CPC分类号： G06F17/243 ,  G06F3/0488

摘要： This disclosure provides a data recording system and, in particular, an electronic digitizing clipboard and a remote computer. A stylus of the clipboard is used to write on pages, with a digitizer underneath the pages generating an electronic record of entered marks for storage and later recreation of the pages. The electronic record represents both stylus spatial coordinates, normally sampled every ten milliseconds, certain user commands such as page identification, inserted at significant locations within the aggregate data stream, or other data, for example, audio data from a built-in microphone. Data in the stream is associated with prior page identification commands, such that subsequent data may be cut or sorted based upon timing of that data relative to the page identification commands. In this manner, data may be distinguished and moved by a "splicing" mechanism, notwithstanding any spatial overlap with previous stylus data.

摘要翻译： 本公开提供了一种数据记录系统，特别是电子数字化剪贴板和远程计算机。 剪贴板的触控笔用于在页面上书写，数字化仪在页面下面生成输入的标记的电子记录，以便随后重新设计页面。 电子记录表示通常每10毫秒采样的指示笔空间坐标，在聚合数据流内的重要位置插入的某些用户命令，例如页面标识，或其它数据，例如来自内置麦克风的音频数据。 流中的数据与先前的页面识别命令相关联，使得可以基于该数据相对于页面标识命令的定时来对后续数据进行切割或排序。 以这种方式，可以通过“拼接”机制来区分和移动数据，尽管与先前的触笔数据具有空间重叠。

公开(公告)号：US08528111B2

公开(公告)日：2013-09-03

申请号：US13176689

申请日：2011-07-05

申请人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

发明人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

IPC分类号： G01Q30/02

CPC分类号： G01Q60/42 ,  G01Q30/025

摘要： A method for positioning a tip of an atomic force microscope relative to a intracellular target site in a cell is provided. In general terms, the method comprises: a) positioning a fluorescent tip of an atomic force microscope over a cell comprising a fluorescent intracellular target site so that said tip is above target site; b) moving the tip toward said target site while obtaining images of the distal end of said tip and/or the target site using a fluorescence microscope; and c) arresting the movement of the tip when the target site and the distal end of the tip are both in focus in the fluorescence microscope. A microscope system for performing the method is also provided.

摘要翻译： 提供了一种用于将原子力显微镜的尖端相对于细胞中的细胞内靶位点定位的方法。 通常来说，该方法包括：a）将原子力显微镜的荧光尖端定位在包含荧光细胞内靶位点的细胞上，使得所述尖端高于目标位点; b）使用荧光显微镜获得所述尖端和/或靶位点的远端的图像，使尖端移向所述目标部位; 以及c）当荧光显微镜中的尖端的目标部位和远端都聚焦时，阻止尖端的运动。 还提供了用于执行该方法的显微镜系统。

公开(公告)号：US08321138B2

公开(公告)日：2012-11-27

申请号：US11193912

申请日：2005-07-29

申请人： Bo U. Curry ,  Jayati Ghosh

发明人： Bo U. Curry ,  Jayati Ghosh

IPC分类号： G01N33/48 ,  C12Q1/68 ,  G06F19/00

CPC分类号： G06F19/20

摘要： Methods, systems, and computer readable media for determining the quality of a CGH array, including calculating a spread of the derivative of log ratio value differences between consecutive probes representing consecutive positions along a chromosome, wherein ratio values are calculated from probe signals from a CGH array.

摘要翻译： 用于确定CGH阵列质量的方法，系统和计算机可读介质，包括计算代表沿着染色体的连续位置的连续探针之间的对数比值差的导数的扩展，其中根据CGH的探测信号计算比值 数组。

公开(公告)号：US20090180925A1

公开(公告)日：2009-07-16

申请号：US12409861

申请日：2009-03-24

申请人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

发明人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

IPC分类号： G01N31/22

CPC分类号： G01N33/54386 ,  G01N21/17 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/8483 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/062 ,  G01N2201/067 ,  Y10S435/805 ,  Y10S435/823 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/25125

摘要： An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.

公开(公告)号：US07521260B2

公开(公告)日：2009-04-21

申请号：US11280640

申请日：2005-11-16

申请人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

发明人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

IPC分类号： G01N33/533

CPC分类号： G01N33/54386 ,  G01N21/17 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/8483 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/062 ,  G01N2201/067 ,  Y10S435/805 ,  Y10S435/823 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/25125

摘要： An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.

摘要翻译： 测定试片包括流路，样品接收区，标签，包括感兴趣区域的检测区和至少一个位置标记。 所述至少一个位置标记相对于所述感兴趣区域对齐，使得所述至少一个位置标记的位置指示所述感兴趣区域的位置。 诊断测试系统包括从测试条的暴露区域获得光强度测量的读取器，以及数据分析器，其执行以下至少一个：（a）基于至少一个（a）识别从测试区域获得的光强度测量中的一个 测量从所述至少一个参考特征获得的，以及（b）基于从所述至少一个参考特征获得的至少一个测量值生成修改所述读取器的至少一个操作参数的控制信号。

公开(公告)号：US20080102453A1

公开(公告)日：2008-05-01

申请号：US11591222

申请日：2006-10-31

申请人： Jayati Ghosh ,  Bo U. Curry

发明人： Jayati Ghosh ,  Bo U. Curry

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G16B25/00

摘要： Methods, systems and computer readable media for analysis of comparative genomic hybridization data analysis, including creating a centralization curve from log ratio data values for DNA copy numbers of a genome of a test sample relative to a genome of a reference sample, wherein the reference sample has a known ploidy, and the test sample has a same copy number as the reference sample in normal, non-aberrant genomic regions; identifying a peak corresponding to regions of normal copy number in the centralization curve; centralizing the log ratio data so that the peak corresponding to regions of normal copy number is centered at a log ratio value of zero; calculating a mathematical measurement that is a function of the width of the peak corresponding to regions of normal copy number; calculating a tolerance value as a function of the mathematical measurement; and outputting the tolerance value. Methods, systems and computer readable media are provided to create a centralization curve from log ratio data values for DNA copy numbers of a genome of a test sample relative to a genome of a reference sample, wherein the reference sample has a known ploidy, and the test sample has a same copy number as the reference sample in normal, non-aberrant genomic regions; identify peaks in the centralization curve; assign copy numbers to the identified peaks; plot expected ratios, based on the assigned copy numbers, of the peaks versus observed ratios of the peaks calculated from the log ratio data values; conclude that the assigned copy numbers are correct if the plot of the expected ratios versus the observed ratios is substantially linear; and output at least one of the plot of expected ratios versus observed ratios, and a conclusion as to whether the plot is substantially linear.

摘要翻译： 用于分析比较基因组杂交数据分析的方法，系统和计算机可读介质，包括从参考样品的基因组的测试样品的基因组的DNA拷贝数的对数比数据值创建中心化曲线，其中参考样品 具有已知的倍性，测试样品与正常非异常基因组区域中的参考样品具有相同的拷贝数; 识别对应于集中曲线中正常拷贝数的区域的峰值; 集中对数比数据使得对应于正常拷贝数的区域的峰值以对数比值为零为中心; 计算作为与正常拷贝数的区域对应的峰值的宽度的函数的数学测量; 计算公差值作为数学测量的函数; 并输出公差值。 提供方法，系统和计算机可读介质以从测试样品的基因组的DNA拷贝数的对数比数据值相对于参照样品的基因组产生中心化曲线，其中参考样品具有已知的倍性， 测试样品与正常非异常基因组区域中的参考样品具有相同的拷贝数; 识别中心曲线中的峰值; 将复制号码分配给识别的峰; 根据分配的拷贝数，从对数比数据值计算的峰与观察到的峰的比率的预期比率; 得出结论，如果预期比率与观察到的比率的曲线基本上是线性的，则分配的拷贝数是正确的; 并输出预期比率与观察到的比率的曲线中的至少一个，以及关于该曲线是否基本上是线性的结论。

公开(公告)号：US5629499A

公开(公告)日：1997-05-13

申请号：US572356

申请日：1995-12-14

申请人： Daniel Flickinger ,  Bo U. Curry

发明人： Daniel Flickinger ,  Bo U. Curry

IPC分类号： G06F3/041 ,  G06F1/16 ,  G06F3/033 ,  G06F3/048 ,  G08C21/00

CPC分类号： G06F3/0488 ,  G06F1/1626 ,  G06F1/163 ,  G06F1/1656 ,  G06F1/1684 ,  G06F1/1698 ,  G06F2200/1632

摘要： A low-cost and rugged electronic apparatus to store and to transfer written information. The apparatus includes a pen and a board, which has a sensing mechanism, a memory device and a communication link. When the pen imprints symbols to the board, the symbols are not concurrently electronically displayed. But the symbols are detected by the sensing mechanism, stored in the memory device, and can later be transferred to an electronic device through the communication link.

摘要翻译： 一种低成本且坚固耐用的电子设备，用于存储和传输书面信息。 该装置包括笔和板，其具有感测机构，存储装置和通信链路。 当笔将符号印在板上时，符号不同时电子显示。 但是，这些符号由感测机构检测，存储在存储器件中，并且可以随后通过通信链路传送到电子设备。

公开(公告)号：US20130014295A1

公开(公告)日：2013-01-10

申请号：US13176689

申请日：2011-07-05

申请人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

发明人： Nazumi Alice Yamada ,  Bo U. Curry ,  Chriatian Rankl

IPC分类号： G01Q10/00

CPC分类号： G01Q60/42 ,  G01Q30/025

摘要： A method for positioning a tip of an atomic force microscope relative to a intracellular target site in a cell is provided. In general terms, the method comprises: a) positioning a fluorescent tip of an atomic force microscope over a cell comprising a fluorescent intracellular target site so that said tip is above target site; b) moving the tip toward said target site while obtaining images of the distal end of said tip and/or the target site using a fluorescence microscope; and c) arresting the movement of the tip when the target site and the distal end of the tip are both in focus in the fluorescence microscope. A microscope system for performing the method is also provided.

摘要翻译： 提供了一种用于将原子力显微镜的尖端相对于细胞中的细胞内靶位点定位的方法。 通常来说，该方法包括：a）将原子力显微镜的荧光尖端定位在包含荧光细胞内靶位点的细胞上，使得所述尖端高于目标位点; b）使用荧光显微镜获得所述尖端和/或靶位点的远端的图像，使尖端移向所述目标部位; 以及c）当荧光显微镜中的尖端的目标部位和远端都聚焦时，阻止尖端的运动。 还提供了用于执行该方法的显微镜系统。

公开(公告)号：US20090180927A1

公开(公告)日：2009-07-16

申请号：US12409877

申请日：2009-03-24

申请人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

发明人： Patrick T. Petruno ,  John F. Petrilla ,  Michael J. Brosnan ,  Rong Zhou ,  Daniel B. Roitman ,  Bo U. Curry

IPC分类号： G01N31/22

CPC分类号： G01N33/54386 ,  G01N21/17 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/8483 ,  G01N33/558 ,  G01N2021/6439 ,  G01N2201/062 ,  G01N2201/067 ,  Y10S435/805 ,  Y10S435/823 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/823 ,  Y10T436/25125

摘要： An assay test strip includes a flow path, a sample receiving zone, a label, a detection zone that includes a region of interest, and at least one position marker. The at least one position marker is aligned with respect to the region of interest such that location of the at least one position marker indicates a position of the region of interest. A diagnostic test system includes a reader that obtains light intensity measurement from exposed regions of the test strip, and a data analyzer that performs at least one of (a) identifying ones of the light intensity measurements obtained from the test region based on at least one measurement obtained from the at least one reference feature, and (b) generating a control signal modifying at least one operational parameter of the reader based on at least one measurement obtained from the at least one reference feature.

摘要翻译： 测定试片包括流路，样品接收区，标签，包括感兴趣区域的检测区和至少一个位置标记。 所述至少一个位置标记相对于所述感兴趣区域对齐，使得所述至少一个位置标记的位置指示所述感兴趣区域的位置。 诊断测试系统包括从测试条的暴露区域获得光强度测量的读取器，以及数据分析器，其执行以下至少一个：（a）基于至少一个（a）识别从测试区域获得的光强度测量中的一个 测量从所述至少一个参考特征获得的，以及（b）基于从所述至少一个参考特征获得的至少一个测量值生成修改所述读取器的至少一个操作参数的控制信号。

公开(公告)号：US06927389B2

公开(公告)日：2005-08-09

申请号：US10309465

申请日：2002-12-03

申请人： Bo U. Curry ,  Jayati Ghosh ,  Kenneth L. Staton

发明人： Bo U. Curry ,  Jayati Ghosh ,  Kenneth L. Staton

IPC分类号： G01N21/64 ,  G02B7/08 ,  H01J3/14 ,  H01J5/16 ,  H01J40/14

CPC分类号： G01N21/6452 ,  G02B7/08

摘要： Optical scanner system approaches are described in which novel focusing approaches are provided. A control algorithm accounts for geometric variation of successive scans in opposite directions across a microarray slide or substrate in order to provide optimized focus. The feedback approach taught may involve PI or PID terms. In either type of control approach, a projected slope of the slide is calculated and followed back and forth outside a scan region of the array in exiting and entering fully adaptive focusing zones, respectively. During turn-around, the system may track a setpoint between the periods of following the extrapolated slope. Also provided are methods of using the subject system in a biopolymer array based application, including genomic and proteomic applications.

摘要翻译： 描述了提供新颖的聚焦方法的光学扫描器系统方法。 控制算法考虑了跨微阵列载玻片或基底的相反方向的连续扫描的几何变化，以便提供优化的焦点。 所教导的反馈方法可能涉及PI或PID术语。 在任一类型的控制方法中，计算滑块的投影斜率并分别在阵列的扫描区域之间来回并进入完全自适应聚焦区域。 在回转期间，系统可以追踪在外推斜率之后的周期之间的设定值。 还提供了在基于生物聚合物阵列的应用中使用主题系统的方法，包括基因组和蛋白质组学应用。