公开(公告)号：US20210115377A1

公开(公告)日：2021-04-22

申请号：US17050734

申请日：2019-05-07

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12M1/12 ,  D01F8/02

Abstract: The present invention relates to an extracellular-matrix-containing composition comprising: a fragmented extracellular matrix component; and an aqueous medium.

公开(公告)号：US20250057974A1

公开(公告)日：2025-02-20

申请号：US18723980

申请日：2022-12-14

Applicant: TOPPAN Holdings Inc. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Masahiko NAKAMOTO ,  Shiro KITANO

IPC: A61K47/69 ,  A61K9/06 ,  A61K47/62 ,  C08J3/24 ,  C08L89/00

Abstract: The present invention relates to a method for changing a hydrogel volume, the method including a step A of bringing an ionic polymer into contact with a hydrogel, which has a site having decomposition activity for the ionic polymer and a cross-linkable functional group that is capable of cross-linking with the ionic polymer through electrostatic interaction, to allow the cross-linkable functional group to cross-link with the ionic polymer, and reducing a volume of the hydrogel; and a step B of decomposing the ionic polymer cross-linking with the hydrogel by using the site having decomposition activity and discharging at least part of a decomposition product of the ionic polymer from the hydrogel to increase the hydrogel volume.

公开(公告)号：US20210063374A1

公开(公告)日：2021-03-04

申请号：US16644059

申请日：2018-09-03

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: G01N33/15 ,  C12N5/077 ,  C07K14/78

Abstract: The present invention relates to an abnormal cardiac rhythm myocardial model composed of a three-dimensional tissue containing cells including the cardiomyocytes and collagen, wherein at least a portion of the cells adheres to the collagen.

公开(公告)号：US20220133952A1

公开(公告)日：2022-05-05

申请号：US17502496

申请日：2021-10-15

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  A61L27/44 ,  A61L27/54 ,  A61L31/04 ,  C12N5/00 ,  A61L27/38

Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.

公开(公告)号：US20190351098A1

公开(公告)日：2019-11-21

申请号：US16480114

申请日：2018-01-31

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  C12N5/00 ,  A61L31/04 ,  A61L27/44 ,  A61L27/54

Abstract: Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.

公开(公告)号：US20250002865A1

公开(公告)日：2025-01-02

申请号：US18704322

申请日：2022-10-27

Applicant: TOPPAN Holdings Inc. ,  OSAKA UNIVERSITY ,  KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: C12N5/071

Abstract: There is provided a method for producing a tissue construct comprising vascular endothelial cells, the method comprising incubating cells comprising at least adipose-derived stem cells from human in the presence of a TGFβ type I receptor inhibitor.

公开(公告)号：US20210238542A1

公开(公告)日：2021-08-05

申请号：US17050731

申请日：2019-05-07

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/00

Abstract: The present invention relates to an extracellular-matrix-containing composition comprising: a fragmented extracellular matrix component, wherein at least a part of the fragmented extracellular matrix component is crosslinked.

公开(公告)号：US20230203451A1

公开(公告)日：2023-06-29

申请号：US18171415

申请日：2023-02-20

Applicant: TOPPAN Inc.

Inventor： Mizuho SUZUKI ,  Shiro KITANO

IPC: C12N5/071

CPC classification number: C12N5/0691 ,  C12N2513/00 ,  C12N2501/91 ,  C12N2533/54 ,  C12N2500/02 ,  C12N2500/60

Abstract: A method for producing a three-dimensional cell structure having a vascular network, including preparing a mixture of a cationic substance, a polyelectrolyte, an extracellular matrix component, and a cell population including endothelial cells, collecting, from the mixture, a cell aggregate including the cell population, the cationic substance, the polyelectrolyte, and the extracellular matrix component, and culturing a collected cell aggregate in a medium. The mixture includes the extracellular matrix at a concentration of 1.0×10−8 mg/mL or more and less than 2.5×10−2 mg/mL.

公开(公告)号：US20230121965A1

公开(公告)日：2023-04-20

申请号：US18069509

申请日：2022-12-21

Applicant: TOPPAN INC. ,  Japanese Foundation For Cancer Research

Inventor： Shiro KITANO ,  Eiji SHINOZAKI

IPC: G06N3/09

Abstract: An evaluation device for evaluating an anticancer effect includes a learning information acquisition unit that acquires learning data that includes state information which indicates at least a cancer state in an unspecified subject and training data that is information about an effect of an anticancer agent obtained by administering the anticancer agent to cells collected from the subject, a learning unit that generates a prediction model by causing a learning model to perform supervised learning for a corresponding relationship between the learning data and the training data, and a prediction unit that makes related predictions to therapy using the anticancer agent with the prediction model. The learning information acquisition unit acquires the information about the anticancer effect obtained by administering the anticancer agent to a three-dimensional cell structure including cancer cells collected from the unspecified subject and cells constituting a stroma as the training data.

公开(公告)号：US20230193211A1

公开(公告)日：2023-06-22

申请号：US18171862

申请日：2023-02-21

Applicant: TOPPAN Inc.

Inventor： Rii MORIMURA ,  Isana NADA ,  Shiro KITANO

IPC: C12N5/071

CPC classification number: C12N5/069 ,  C12N2513/00 ,  C12N2533/50 ,  C12N2533/90

Abstract: A method for producing a three-dimensional cell structure includes preparing a mixture of a cationic substance, an extracellular matrix component, a polyelectrolyte, and a cell population including endothelial cells and mouse-derived stromal cells, which exclude mouse-derived endothelial cells, collecting a cell aggregate from the mixture, and culturing a collected cell aggregate to obtain a three-dimensional cell structure.