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11.发明授权公开(公告)号：US08529889B2
公开(公告)日：2013-09-10
申请号：US11171490
申请日：2005-06-29
申请人： Aarthi Chandrasekaran , Shiladitya Sengupta , David A. Berry , Kristine Holley , Ganlin Zhao , Ram Sasisekharan
发明人： Aarthi Chandrasekaran , Shiladitya Sengupta , David A. Berry , Kristine Holley , Ganlin Zhao , Ram Sasisekharan
IPC分类号： A61K38/47
CPC分类号： A61K38/51 , A61K31/00 , A61K31/726 , A61K31/727 , A61K31/737 , A61K38/45 , A61K45/06 , A61K47/36 , A61K2300/00
摘要： The invention relates to compositions and methods for the modulation of the permeability of the epithelial cell barrier complex. In particular, the invention provides compositions and methods for using polysaccharides, preferably glycosaminoglycans, and agents that modify cell surface glycosaminoglycans, preferably glycosaminoglycan-degrading enzymes to modulate intercellular junctions. The compositions and methods provided can be used to facilitate the delivery of biologically active molecules.
摘要翻译： 本发明涉及调节上皮细胞屏障复合物渗透性的组合物和方法。 特别地，本发明提供使用多糖，优选糖胺聚糖的组合物和方法，以及修饰细胞表面糖胺聚糖（优选为糖胺聚糖降解酶）以调节细胞间连接的试剂。 所提供的组合物和方法可用于促进生物活性分子的递送。
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12.发明申请Methods and compositions related to modulating the extracellular stem cell environment 审中-公开与调节细胞外干细胞环境相关的方法和组合公开(公告)号：US20070020243A1
公开(公告)日：2007-01-25
申请号：US11332766
申请日：2006-01-12
申请人： Shiladitya Sengupta , Ram Sasisekharan , Nishla Keiser , David Eavarone , Tanyel Kiziltepe Bilgicer , Aarthi Chandrasekaran , David Berry , Kristine Holley
发明人： Shiladitya Sengupta , Ram Sasisekharan , Nishla Keiser , David Eavarone , Tanyel Kiziltepe Bilgicer , Aarthi Chandrasekaran , David Berry , Kristine Holley
CPC分类号： A61K31/726 , A61K31/727 , A61K38/47 , C12N5/0606 , C12N5/0691 , C12N2501/70 , C12N2506/02
摘要： This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Such modulation, in some aspects of the invention, is accomplished by agents that modulate glycosaminoglycans in the stem cell microenvironment (i.e., at or on the cell surface and/or in the extracellular matrix). Therefore, methods and compositions are provide for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided. Furthermore, the invention relates, in part, to tissues, and uses thereof, formed by the methods and compositions provided. Moreover, the invention also relates, in part, to methods of treatment using the methods and compositions provided.
摘要翻译： 本发明部分涉及调节干细胞环境的方法和组合物。 更具体地，本发明部分涉及用于调节干细胞分化的方法和组合物。 在本发明的某些方面，这种调节是通过调节干细胞微环境中的糖胺聚糖（即在细胞表面上和/或细胞外基质中）上的调节剂来完成的。 因此，提供了在干细胞的微环境中调节糖胺聚糖部分，例如硫酸乙酰肝素糖胺聚糖（HSGAG）部分的方法和组合物。 还提供了用于促进或抑制胚胎干细胞分化（例如分化成内皮细胞）的方法和组合物。 因此，本发明还部分地涉及可以用所提供的方法和组合物产生的细胞群体（例如，内皮细胞群体或贫血的内皮细胞群体）。 此外，本发明部分地涉及通过所提供的方法和组合物形成的组织及其用途。 此外，本发明还部分涉及使用提供的方法和组合物的治疗方法。
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公开(公告)号：US10131903B2
公开(公告)日：2018-11-20
申请号：US13437727
申请日：2012-04-02
摘要： This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.
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公开(公告)号：US20120258487A1
公开(公告)日：2012-10-11
申请号：US13437727
申请日：2012-04-02
CPC分类号： C12N15/1027 , C12N15/10 , C12N15/66
摘要： This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of said biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to for a sequenced order of droplets carrying different components of said biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of said biological construct to permit said components to react with each other in one or more reactions contributing to the assembly of said biological construct.
摘要翻译： 本发明提供用于组装生物构建体（例如质粒，转化细胞等）的方法和组合物。 在某些实施方案中，所述方法包括将所述生物构建体的单独组分封装在限制在流体通道中的流体液滴中; 任选地将来自不同流体通道的液滴混合以便在通道或室中携带所述生物构建体的不同组分的液滴的顺序排列; 并且任选地组合两个或更多个液滴，每个液滴包含所述生物构建体的不同组分，以允许所述组分在有助于所述生物构建体的组装的一个或多个反应中彼此反应。
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