Method for the rapid detection of microorganisms in samples
    15.
    发明授权
    Method for the rapid detection of microorganisms in samples 失效
    快速检测样品中微生物的方法

    公开(公告)号:US5660998A

    公开(公告)日:1997-08-26

    申请号:US741533

    申请日:1991-09-27

    IPC分类号: C12M1/34 C12Q1/04 C12Q1/24

    摘要: The invention relates to a method for the rapid detection and enumeration of microorganisms. The spectroscopic detection is carried out, after brief incubation of the sample to be tested, by the detection of the growth of microcolonies, which is not yet detectable with the naked eye, on a solid culture plate following direct transfer of said microcolonies to a sample carrier by special impression techniques; the detection itself is carried out by recording the spectra of all, or of a representative number, of the impressions, using an IR microscope. It is not only possible to make statements with regard to the number of microorganisms in the sample but the high information content of the spectra can also be used for demanding microbiological differentiation work down to species identification.

    摘要翻译: PCT No.PCT / DE90 / 00081 Sec。 371日期1991年9月27日 102(e)1991年9月27日PCT提交1990年2月8日PCT公布。 出版物WO90 / 09453 日期1990年8月23日本发明涉及微生物快速检测和计数方法。 通过在将所述微菌落直接转移到样品后的固体培养板上,通过检测肉眼尚不可检测到的微菌落的生长,在待测样品短暂孵育后进行光谱检测 通过特殊印象技术承载; 检测本身通过使用IR显微镜记录全部或代表性数字的印象的光谱来进行。 不仅可以对样品中的微生物数量作出陈述,而且光谱的高信息含量也可以用于要求微生物分化的作物到物种鉴定。